Purification of a fibrinolytic enzyme (myulchikinase) from pickled anchovy and its cytotoxicity to the tumor cell lines

Biotechnology Letters

Volumn 26, Issue 5, 2004, Pages 393-397

  Jeong, Yong Kee ^a   Yang, Woong Suk ^a   Kim, Kwang Hyuk ^b   Chung, Kyung Tae ^a   Joo, Woo Hong ^c   Kim, Jae Hyun ^d   Kim, Dong Eun ^a   Park, Jeong Uck ^e  

^a Dong Eui University   (South Korea)

^b Kosin University College of Medicine   (South Korea)

^c Changwon National University   (South Korea)

^d Chungbuk National University   (South Korea)

^e Gyeongsang National University School of Medicine   (South Korea)

Author keywords

Amidolytic activity; Cytotoxicity; Fibrinolytic enzyme; Myulchikinase

Indexed keywords

ANTINEOPLASTIC AGENT; FIBRINOLYTIC AGENT; FIXATIVE; MYULCHIKINASE; PROTEINASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BREAST; CELL STRAIN K 562; CELL SURVIVAL; CHEMISTRY; COMPARATIVE STUDY; DOSE RESPONSE; DRUG EFFECT; ENZYME ACTIVATION; EPITHELIUM CELL; EVALUATION; FISH; HUMAN; ISOLATION AND PURIFICATION; LYMPHOMA; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; MOUSE; NEOPLASM; PATHOLOGY; SEQUENCE HOMOLOGY; TUMOR CELL LINE;

AMINO ACID SEQUENCE; ANIMALS; ANTINEOPLASTIC AGENTS; BREAST; CELL LINE, TUMOR; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOPEPTIDASES; ENZYME ACTIVATION; EPITHELIAL CELLS; FIBRINOLYTIC AGENTS; FISHES; FIXATIVES; HUMANS; K562 CELLS; LYMPHOMA; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; NEOPLASMS; SEQUENCE HOMOLOGY, AMINO ACID;

ANCHOA; ANIMALIA; ASTEROIDEA;

EID: 1642343002     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000018257.18617.6d     Document Type: Article

References (13)

1. Altschul SF, Madden TL, Scaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25: 3389-3402.
2. Astrup T, Müllertz S (1952) The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40: 346-351.
3. Espevik T, Nissen-Meyer J (1986) A highly sensitive cell line, WEHI164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95: 99-105.
4. Estell DA, Laskowski Jr. M (1980) Dermasterias imbricata trypsin 1: an enzyme which rapidly hydrolyzes the reactive-site peptide bonds of protein trypsin inhibitors. Biochemistry 19: 124-131.
5. Jacobs M, Eliasson M, Uhlen M, Flock JI (1985) Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucl. Acids Res. 13: 8913-8926.
6. Kim WK, Choi K, Kim Y, Park H, Choi J, Lee Y, Oh H, Kwon I, Lee S (1996) Purification and characterization of a fibrinolytic enzyme produced from Bacillus strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62: 2482-2488.
7. Liotta LA, Steeg PS, Stetler-Stevenson WG (1991) Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell 64: 327-336.
8. Park YD, Kim LW, Min BG, Seo JW, Jeong JM (1998) Rapid purification and biochemical characteristics of lumbrokinase III from earthworm for use as a fibrinolytic agent. Biotechnol. Lett. 20: 169-172.
9. Sumi H, Hamada H, Nakanishi K, Hiratani H (1990) Enhancement of the fibrinolytic activity in plasma by oral administration of NK. Acta Haematol. 84: 139-143.
10. Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H (1987) A novel fibrinolytic enzyme (Nattokinase) in the vegetable cheese natto: a typical and popular soybean food in the Japanese diet. Experimentia 43: 1110-1111.
11. Sumi H, Nakajima N, Yatagai C (1995) A unique strong fibrinolytic enzyme (katsuwokinase) in skipjack 'Shiokara', a Japanese traditional fermented food. Comp. Biochem. Physiol. 112: 543-547.
12. Szilagyi CM, Sarfati P, Pradayrol L, Morisset J (1995) Purification, characterization and substrate specificity of rat pancreatic elastase II. Biochim. Biophys. Acta. 1251: 55-65.
13. Toki N, Sumi H, Sasaki K, Boreisha I, Robbins KC (1985) Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins. J. Clin. Invest. 75: 1212-1222.