Transplanting fragments of diabetic pancreas into activated omentum gives rise to new insulin producing cells

Biochemical and Biophysical Research Communications

Volumn 355, Issue 1, 2007, Pages 258-262

  Singh, Ashok K ^a,c   Gudehithlu, Krishnamurthy P ^a   Litbarg, Natalia O ^c   Sethupathi, Perianna ^c   Arruda, Jose A L ^a,b   Dunea, George ^a,c  

^a Jr Hospital of Cook County   (United States)

^b UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^c HEKTOEN INSTITUTE OF MEDICINE   (United States)

Author keywords

Cells; Auto transplantation; Diabetes; Growth factors; Insulin; Omentum; Pancreas; Pdx 1; Progenitors; Streptozotocin

Indexed keywords

GLUCOSE; GROWTH FACTOR; INSULIN; STREPTOZOCIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTOTRANSPLANTATION; CELL ACTIVATION; CELL FUNCTION; CONTROLLED STUDY; DIABETES MELLITUS; FOREIGN BODY; GLUCOSE BLOOD LEVEL; HORMONE SYNTHESIS; INSULIN SYNTHESIS; MEASUREMENT; NONHUMAN; OMENTUM; PANCREAS; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; RAT; STEM CELL;

ANIMALS; DIABETES MELLITUS, EXPERIMENTAL; INSULIN; MALE; OMENTUM; PANCREAS TRANSPLANTATION; PANCREATECTOMY; RATS; RATS, SPRAGUE-DAWLEY; TRANSPLANTATION, AUTOLOGOUS;

ANIMALIA;

EID: 33847141541     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.01.152     Document Type: Article

References (27)

1. Stock P.G., and Bluestone J.A. Beta-cell replacement for type I diabetes. Annu. Rev. Med. 55 (2004) 133-156
2. DeVos P., Jeanette F.M., Straaten V., et al. Why do microencapsulated islet grafts fail in the absence of fibrotic overgrowth?. Diabetes 48 (1999) 1381-1388
3. Ramiya V.K., Maraist M., Arfors K.E., et al. Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells. Nature Medicine 6 (2000) 278-282
4. Bonner-Weir S., Taneja M., Weir G., et al. In vitro cultivation of human islets from expanded ductal tissue. Proc. Natl. Acad. Sci. USA 97 (2000) 7999-8004
5. Gao R., Ustinov J., Pulkkinen M.-A., et al. Characterization of endocrine progenitor cells and critical factors for their differentiation in human adult pancreatic cell culture. Diabetes 52 (2003) 2007-2015
6. Kodama S., Kuhtreiber W., Fujimura S., Dale E.A., and Faustman D.L. Islet regeneration during the reversal of autoimmune diabetes in NOD mice. Science 302 (2003) 1223-1227
7. Chong A.S., Shen J., Tao J., Yin D., Kuznetsov A., Hara M., and Philipson L.H. Reversal of diabetes in non-obese diabetic mice without spleen cell-derived β cell regeneration. Science 311 (2006) 1774-1775
8. Nishio J., Galglia J.L., Turvey S.E., Campbell C., Benoist C., and Mathis D. Islet recovery and reversal of murine type 1 diabetes in the absence of any infused spleen cell contribution. Science 311 (2006) 1775-1778
9. Suri A., Calderon B., Esparza TJ., Frederick K., Bittner P., and Unanue ER. Immunological reversal of autoimmune diabetes without hematopoietic replacement of β cells. Science 311 (2006) 1778-1780
10. In: Goldsmith H.S. (Ed). The Omentum: Application to Brain and Spinal cord, Wilton CT (2000), Forefront Publishing
11. Liebermann-Meffert D. The greater omentum: anatomy, embryology, and surgical applications. Surg. Clin. North America 80 (2000) 275-293
12. Goldsmith H.S. The evolution of omentum transposition: from lymphedema to spinal cord, stroke and Alzheimer's disease. Neurol. Res. 26 (2004) 586-593
13. Cannaday J.E. Some uses of undetached omentum in surgery. Am. J. Surg. 76 (1948) 502-505
14. Zhang Q.-X., Magovern C.J., Mack C.A., Budenbender K.T., Wilson K., and Rosengart T.K. Vascular endothelial growth factor is the major angiogenic factor in omentum: mechanism of the omentum-mediated angiogenesis. J. Surg. Res. 67 (1997) 147-154
15. Mydlo J.H., Kral J.G., and Macchia R.J. Preliminary results comparing the recovery of basic fibroblast growth factor (FGF-2) in adipose tissue and benign and malignant renal tissue. J. Urol. 159 (1998) 2159-2163
16. Fatima B., Pinho M., Hurtado S.P., El-Cheikh M.C., and Borojevic R. Hematopoietic progenitors in the adult mouse omentum: permanent production of B lymphocytes and monocytes. Cell Tissue Res. 319 (2005) 91-102
17. N.O. Litbarg, K.P. Gudehithlu, P. Sethupathi, J.A.L. Arruda, G. Dunea, A.K. Singh, Activated omentum becomes rich in factors that promote healing and tissue regeneration, Cell Tissue Res. (2007), in press.
18. Controls consisted of (1) PD-activated diabetic rats from which the pancreas were removed but not transplanted and so discarded (non-transplanted group II), (2) non PD- activated diabetic rats in which pancreas were auto-transplanted without polydextran particles (non-activated, transplanted group III), (3) PD-activated diabetic rats in which pancreas were not removed, and therefore not auto-transplanted (STZ diabetic rats with activated omentum, group IV) and (4) normal non-diabetic rats without PD-activation of omentum and without pancreatectomy and auto-transplantation, but sham laparotomized (normal sham control group V) (n = 4 in each of group II-V).
19. Additional controls included (1) normal rats (for harvesting normal pancreas) (n = 4, same as group V), (2) normal rats in which omentum was activated by injection of PD (for harvesting normal activated omentum; group VI, n = 4), and (3) 6 week STZ diabetic rats without PD-activation of omentum (for harvesting diabetic pancreas; group VII, n = 4).
20. Image analysis was performed on ten random pictures of immunocytochemically stained tissue using the ImageJ software (JAVA imaging software inspired by the National Institute of Health and available free at http://rsb.info.nih.gov).
21. Ferber S., Halkin A., Cohen H., et al. Pancreatic and duodenal homeobox-1 gene induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia. Nat. Med. 6 (2000) 568-572
22. Narushima M., Kobayashi N., Okitsu T., et al. A human β-cell line for transplantation therapy to control type 1 diabetes. Nat. Biotechnol. 23 (2005) 1274-1282
23. Kin T., Korbutt G.S., and Rajotte R.V. Survival of islets in omental pouch. Am. J. Transplant. 3 (2003) 281-285
24. Hammerman M.R. Renal organogenesis from transplanted metanephric primordial. J. Am. Soc. Nephrol. 15 (2004) 1126-1132
25. Leahy J.L., Bumbalo L.M., and Chen C. Beta-cell hypersensitivity for glucose precedes loss of glucose-induced insulin secretion in 90% pancreatectomized rats. Diabetologia 36 (1993) 1238-1244
26. Liu Y.Q., Nevin P.W., and Leahy J.L. β-cell adaptation in the 60 % pancreatectomized rats that preserves normoinsulinemia and normoglycemia. Am. J. Physiol. Endocrinol. Metab. 278 (2000) E68-E73
27. Dor Y., Brown J., Martinez O.I., and Melton D.A. Adult pancreatic β-cells are formed by self-duplication rather then stem-cell differentiation. Nature 429 (2004) 41-46