Cardiac-specific overexpression of SCN5A gene leads to shorter P wave duration and PR interval in transgenic mice

Biochemical and Biophysical Research Communications

Volumn 355, Issue 2, 2007, Pages 444-450

  Zhang, Teng ^a,b   Yong, Sandro L ^a,b   Tian, Xiao Li ^a,b   Wang, Qing K ^a,b  

^a LERNER RESEARCH INSTITUTE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cardiac sodium channel gene SCN5A; Electrocardiogram (ECG); Long QT syndrome (LQTS); P wave and PR interval; Sodium current; Transgenic mice; Ventricular arrhythmias or tachycardia (VT)

Indexed keywords

GENOMIC DNA; RNA 18S; SODIUM CHANNEL;

ANIMAL EXPERIMENT; ARTICLE; ELECTROCARDIOGRAM; GENE; GENE OVEREXPRESSION; HEART FUNCTION; HEART MUSCLE CELL; HEART MUSCLE CONDUCTION DISTURBANCE; HEART MUSCLE POTENTIAL; HEART VENTRICLE ARRHYTHMIA; HEART VENTRICLE TACHYCARDIA; KNOCKOUT MOUSE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; P WAVE; PHENOTYPE; PR INTERVAL; PRIORITY JOURNAL; QT PROLONGATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCN5A GENE; SODIUM CURRENT; SOUTHERN BLOTTING; TELEMETRY; TISSUE SPECIFICITY; TRANSGENE; TRANSGENIC MOUSE;

ACTION POTENTIALS; ANIMALS; ARRHYTHMIA; ELECTROCARDIOGRAPHY; GENE EXPRESSION; HEART; HEART RATE; HUMANS; MICE; MICE, TRANSGENIC; PHENOTYPE; SODIUM CHANNELS;

MUS; MUS MUSCULUS;

EID: 33847111638     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.01.170     Document Type: Article

References (21)

1. Chen Q., Kirsch G.E., Zhang D., Brugada R., Brugada J., Brugada P., Potenza D., Moya A., Borggrefe M., Breithardt G., Ortiz-Lopez R., Wang Z., Antzelevitch C., O′Brien R.E., Schulze-Bahr E., Keating M.T., Towbin J.A., and Wang Q. Genetic basis and molecular mechanism for idiopathic ventricular fibrillation. Nature 392 (1998) 293-296
2. Gellens M.E., George Jr. A.L., Chen L.Q., Chahine M., Horn R., Barchi R.L., and Kallen R.G. Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel. Proc. Natl. Acad. Sci. USA 89 (1992) 554-558
3. Tian X.L., Yong S.L., Wan X., Wu L., Chung M.K., Tchou P.J., Rosenbaum D.S., Van Wagoner D.R., Kirsch G.E., and Wang Q. Mechanisms by which SCN5A mutation N(1325)S causes cardiac arrhythmias and sudden death in vivo. Cardiovasc. Res. 61 (2004) 256-267
4. Wang Q., Shen J., Li Z., Timothy K., Vincent G.M., Priori S.G., Schwartz P.J., and Keating M.T. Cardiac sodium channel mutations in patients with long QT syndrome, an inherited cardiac arrhythmia. Hum. Mol. Genet. 4 (1995) 1603-1607
5. Wang Q., Shen J., Splawski I., Atkinson D., Li Z., Robinson J.L., Moss A.J., Towbin J.A., and Keating M.T. SCN5A mutations associated with an inherited cardiac arrhythmia, long QT syndrome. Cell 80 (1995) 805-811
6. Wu L., Nishiyama K., Hollyfield J.G., and Wang Q. Localization of Nav1.5 sodium channel protein in the mouse brain. Neuroreport 13 (2002) 2547-2551
7. Wang Q., Li Z., Shen J., and Keating M.T. Genomic organization of the human SCN5A gene encoding the cardiac sodium channel. Genomics 34 (1996) 9-16
8. Tan H.L., Bink-Boelkens M.T.E., Bezzina C.R., Viswanathan P.C., Beaufort-Krol G.C.M., Van Tintelen P.J., Van den Berg M.P., Wilde A.A.M., and Balser J.R. A sodium-channel mutation causes isolated cardiac conduction disease. Nature 409 (2001) 1047
9. Wolf C.M., and Berul C.I. Inherited conduction system abnormalities--one group of diseases, many genes. J. Cardiovasc. Electrophysiol. 17 (2006) 446-455
10. Nuyens D., Stengl M., Dugarmaa S., Rossenbacker T., Compernolle V., Rudy Y., Smits J.F., Flameng W., Clancy C.E., Moons L., Vos M.A., Dewerchin M., Benndorf K., Collen D., Carmeliet E., and Carmeliet P. Abrupt rate accelerations or premature beats cause life-threatening arrhythmias in mice with long-QT3 syndrome. Nat. Med. 7 (2001) 1021-1027
11. Remme C.A., Verkerk A.O., Nuyens D., Van Ginneken A.C., van B.S., Belterman C.N., Wilders R., van Roon M.A., Tan H.L., Wilde A.A., Carmeliet P., De Bakker J.M., Veldkamp M.W., and Bezzina C.R. Overlap syndrome of cardiac sodium channel disease in mice carrying the equivalent mutation of human SCN5A-1795insD. Circulation 114 (2006) 2584-2594
12. Papadatos G.A., Wallerstein P.M., Head C.E., Ratcliff R., Brady P.A., Benndorf K., Saumarez R.C., Trezise A.E., Huang C.L., Vandenberg J.I., Colledge W.H., and Grace A.A. Slowed conduction and ventricular tachycardia after targeted disruption of the cardiac sodium channel gene Scn5a. Proc. Natl. Acad. Sci. USA 99 (2002) 6210-6215
13. Royer A., van Veen T.A., Le B.S., Marionneau C., Griol-Charhbili V., Leoni A.L., Steenman M., van Rijen H.V., Demolombe S., Goddard C.A., Richer C., Escoubet B., Jarry-Guichard T., Colledge W.H., Gros D., De Bakker J.M., Grace A.A., Escande D., and Charpentier F. Mouse model of SCN5A-linked hereditary Lenegre's disease: age-related conduction slowing and myocardial fibrosis. Circulation 111 (2005) 1738-1746
14. van Veen T.A., Stein M., Royer A., Le Q.K., Charpentier F., Colledge W.H., Huang C.L., Wilders R., Grace A.A., Escande D., De Bakker J.M., and van Rijen H.V. Impaired impulse propagation in Scn5a-knockout mice: combined contribution of excitability, connexin expression, and tissue architecture in relation to aging. Circulation 112 (2005) 1927-1935
15. Tian X.L., and Wang Q.K. Generation of transgenic mice for cardiovascular research. Methods Mol. Med. 129 (2006) 69-81
16. Tian X.L., Kadaba R., You S.A., Liu M., Timur A.A., Yang L., Chen Q., Szafranski P., Rao S., Wu L., Housman D.E., DiCorleto P.E., Driscoll D.J., Borrow J., and Wang Q. Identification of an angiogenic factor that when mutated causes susceptibility to Klippel-Trenaunay syndrome. Nature 427 (2004) 640-645
17. You S.A., Archacki S.R., Angheloiu G., Moravec C.S., Rao S., Kinter M., Topol E.J., and Wang Q. Proteomic approach to coronary atherosclerosis shows ferritin light chain as a significant marker: evidence consistent with iron hypothesis in atherosclerosis. Physiol. Genomics 13 (2003) 25-30
18. Yong S.L., and Wang Q.K. Animal models for cardiac arrhythmias. Methods Mol. Med. 129 (2006) 127-148
19. Yong S.L., Ni Y., Zhang T., Tester D.J., Ackerman M.J., and Wang Q.K. Characterization of the cardiac sodium channel SCN5A mutation, N(1325)S, in single murine ventricular myocytes. Biochem. Biophys. Res. Commun. 352 (2007) 378-383
20. Tian X.L., Cheng Y., Zhang T., Liao M.L., Yong S.L., and Wang Q.K. Optical mapping of ventricular arrhythmias in LQTS mice with SCN5A mutation N1325S. Biochem. Biophys. Res. Commun. 352 (2007) 879-883
21. Sidhu J.S., Rajawat Y.S., Rami T.G., Gollob M.H., Wang Z., Yuan R., Marian A.J., DeMayo F.J., Weilbacher D., Taffet G.E., Davies J.K., Carling D., Khoury D.S., and Roberts R. Transgenic mouse model of ventricular preexcitation and atrioventricular reentrant tachycardia induced by an AMP-activated protein kinase loss-of-function mutation responsible for Wolff-Parkinson-White syndrome. Circulation 111 (2005) 21-29