Bioavailability and toxicity of pentachlorophenol in contaminated soil evaluated on coelomocytes of Eisenia andrei (Annelida: Lumbricidae)

Toxicology in Vitro

Volumn 21, Issue 2, 2007, Pages 302-307

  Vernile, Pasqua ^a   Fornelli, Francesca ^b   Bari, Giuseppe ^a   Spagnuolo, Matteo ^a   Minervini, Fiorenza ^b   de Lillo, Enrico ^a   Ruggiero, Pacifico ^a  

^a UNIVERSITY OF BARI   (Italy)

^b INSTITUTE OF SCIENCES OF FOOD PRODUCTION   (Italy)

Author keywords

Bioassays; Bioavailability; Coelomocytes; Flow cytometry; PCP

Indexed keywords

ALCOHOL; PENTACHLOROPHENOL;

ANIMAL CELL; ANIMAL EXPERIMENT; ANNELID WORM; ARTICLE; BIOAVAILABILITY; CELL SUBPOPULATION; CELLULAR DISTRIBUTION; CYTOTOXICITY; FLOW CYTOMETRY; IN VIVO STUDY; LYSOSOME MEMBRANE; MEMBRANE DAMAGE; MEMBRANE STABILIZATION; MORTALITY; NONHUMAN; SOIL POLLUTION;

ANIMALS; BIOLOGICAL AVAILABILITY; NEUTRAL RED; OLIGOCHAETA; PENTACHLOROPHENOL; SOIL POLLUTANTS;

ANNELIDA; EISENIA ANDREI; LUMBRICIDAE;

EID: 33846850212     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2006.09.026     Document Type: Article

References (21)

1. Alexander M. Aging, bioavailability, and overestimation of risk from environmental pollutants. Environmental Science and Technology 34 (2000) 4259-4265
2. Agency for Toxic Substances and Disease Registry(ATSDR), 2001. Toxicological profile for pentachlorophenol. Available from: .
3. Baveye P., and Bladon R. Bioavailability of organic xenobiotics in the environment: a critical perspective. In: Baveye P., Block J.C., and Goncharuk V.V. (Eds). Bioavailability of Organic Xenobiotics in the Environment (1999), Kluver Academic Publishers, Amsterdam, The Netherlands 225-246
4. Brousseau P., Dunier M., Deschaux P., De Guise S., Krzystyniak K., and Fournier M. Marquers immunologiques. In: Lagadic L., Caquet T., Amiard J.C., and Ramade F. (Eds). Biomarquers en Écotoxicologie. Aspects fondamentaux (1997), Masson S.A., Paris 287-314
5. Brousseau P., Fugère N., Bernier J., Coderre D., Nadeau D., Poirie G., Dunier M., Deschaux P., De Guise S., Krzystyniak K., and Fournier M. Evaluation of earthworm exposure to contaminated soil by cytometric assay of coelomocytes phagocytosis in Lumbricus terrestris (Oligochaeta). Soil Biology and Biochemistry 29 (1997) 681-684
6. Eason C.T., Svendsen C., O'Halloran K., and Weeks J.M. An assessment of the lysosomal neutral red retention test and immune function assay in earthworms (Eisenia andrei) following exposure to chlorpyrifos, benzo-a-pyrene (BaP), and contaminated soil. Pedobiologia 43 (1999) 641-645
7. Edwards C.A. The importance of earthworms as key representatives of the soil fauna. In: Edwards C.A. (Ed). Earthworm ecology. second ed. (2004), CRC Press, Boca Raton, Florida 3-11
8. Ehrlich W. The effect of pentachlorophenol and its metabolite tetrachlorohydroquinone on cell growth and the induction of DNA damage in Chinese hamster ovary cells. Mutation Research 244 (1990) 299-302
9. Engelmann P., Molnár L., Pálinkás L., Cooper E.L., and Németh P. Earthworm leukocyte populations specifically harbour lysosomal enzymes that may respond to bacterial challenge. Cell and Tissue Research 316 (2004) 391-401
10. Eyambe G.S., Goven A.J., Fitzpatrick L.C., Venables B.J., and Cooper E.L. A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies. Laboratory Animals 25 (1991) 61-67
11. Fernández Freire P., Labrador V., Pérez Martín J.M., and Hazen M.J. Cytotoxic effects in mammalian Vero cells exposed to pentachlorophenol. Toxicology 210 (2005) 37-44
12. Hendawi M., Sauvé S., Ashou M., Brousseau P., and Fournier M. A new ultrasound protocol for extrusion of coelomocyte cells from the earthworm Eisenia fetida. Ecotoxicology Environmental Safety 59 (2004) 17-22
13. Kelsey J.W., Kottler B.D., and Alexander M. Selective chemical extractants to predict bioavailability of soil-aged organic chemicals. Environmental Science and Technology 31 (1997) 214-217
14. Luthy R.G., Aiken G.R., Brusseau M.L., Cunningham S.D., Gschwend P.M., Pignatello J.J., Reinhard M., Traina S.J., Weber Jr. W.J., and Westall J.C. Sequestration of hydrophobic organic contaminants by geosorbents. Environmental Science and Technology 31 (1997) 3341-3347
15. Maboeta M.S., Reinecke S.A., and Reinecke A.J. The relation between lysosomal biomarker and population responses in a field of Microchaetus sp. (Oligochaeta) exposed to the fungicide copper oxychloride. Ecotoxicology Environmental Safety 52 (2002) 280-287
16. OECD, 2000. OECD Guideline for the Testing of Chemicals. Earthworm reproduction Test (Eisenia fetida/andrei) Draft Document, January 2000.
17. Reinecke A.J., and Reinecke S.A. Earthworms as test organisms in ecotoxicological assessment of toxicant impacts on ecosystems. In: Edwards C.A. (Ed). Earthworm ecology. second ed. (2004), CRC Press, Boca Raton, Florida 299-320
18. Spagnuolo, M., Bari, G., de Lillo, E., Pizzigallo, M.D.R., Ruggiero, P., 2004. Influenza dei processi di invecchiamento sulla biodisponibilità potenziale e reale di fenantrene in suoli contaminati. In Morselli, L. Norme, tecnologie e controlli ambientali:acqua, siti contaminati, atmosfera, politiche energetiche, certificazioni ambientali. Maggioli Editore, Rimini, Italy, vol. 312-2, pp. 338-344.
19. Terzano R., Spagnuolo M., Medici L., Tateo F., and Ruggiero P. Zeolite synthesis from pre-treated coal fly ash in presence of soil as a tool for soil remediation. Applied Clay Science 29 2 (2005) 99-110
20. Van Gestel C.A.M., Dirven-van Breemen E.M., Baerselman R., Emans H.J.B., Janssen J.A.M., Postuma R., and Van Vliet P.J.M. Comparison of sublethal and lethal criteria for nine different chemicals in standardized toxicity tests using the earthworm Eisenia andrei. Ecotoxicology and Environmental Safety 23 (1992) 206-220
21. Wang Y.J., Ho Y.S., Jeng J.H., Su H.J., and Lee C.C. Different cell death mechanisms and gene expression in human cells induced by pentachlorophenol and its major metabolite, tetrachlorohydroquinone. Chemico-Biological Interactions 128 (2000) 173-188