A new ultrasound protocol for extrusion of coelomocyte cells from the earthworm Eisenia fetida

Ecotoxicology and Environmental Safety

Volumn 59, Issue 1, 2004, Pages 17-22

  Hendawi, M ^a,b   Sauve S ^c   Ashour, M ^b   Brousseau, P ^a   Fournier, Michel ^a  

^a INRS INSTITUT ARMAND FRAPPIER   (Canada)

^b Zagazig University   (Egypt)

^c UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Earthworms; Immunotoxicology; Invertebrates; Mercury; Phagocytosis

Indexed keywords

ALCOHOL; METHYLMERCURIC CHLORIDE; PROPIDIUM IODIDE;

BIOMARKER; EARTHWORM; ECOTOXICOLOGY; SOIL POLLUTION; XENOBIOTICS;

ANALYTIC METHOD; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; EARTHWORM; ECOTOXICITY; FLOW CYTOMETRY; INTERMETHOD COMPARISON; NONHUMAN; PHAGOCYTOSIS; ULTRASOUND;

ANIMALS; CELL SEPARATION; CELL SURVIVAL; CELLS, CULTURED; ELECTRIC STIMULATION; ETHANOL; FLOW CYTOMETRY; METHYLMERCURY COMPOUNDS; OLIGOCHAETA; PHAGOCYTOSIS; ULTRASONICS;

EISENIA FETIDA; INVERTEBRATA; PHERETIMA SIEBOLDI;

EID: 4644262694     PISSN: 01476513     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ecoenv.2003.07.015     Document Type: Article

References (28)

1. Bilej M., Scheerlinck J.-P., VandenDriessche T., De Baetselier P., Vetvicka V. The flow cytometric analysis of in vitro phagocytic activity of earthworm coelomocytes (Eisenia foetida; annelida). Cell Biology International Reports. 14(9):1990;831-837
2. Brousseau P., Dunier M., De Guise S., Fournier M. Marqueurs immunologiques. Ramade J.F., et al. Biomarqueurs en Écotoxicologie, Aspects Fondamentaux. 1997;287-315 Masson, Paris
3. Brousseau P., Fugère N., Bernier J., Coderre D., Nadeau D., Poirier G., Fournier M. Evaluation of earthworm exposure to contaminated soil by cytometric assay of coelomocytes phagocytosis in Lumbricus terrestris (Oligochaeta). Soil Biol. Biochem. 29:1997;681-684
4. Brousseau P., Payette Y., Tryphonas H., Blakley B., Boermans H., Flipo D., Fournier M. Manual of Immunological Methods. 1999;CRC Press, Boca Raton, FL
5. Chen Sc., Fitzpartrick L.C., Goven A.J., Venables B.J., Cooper E.L. Nitroblue tetrazolium dye reduction by earthworms (Lumbricus terrestris) coelomocytes. an enzymes assay for non-specific immunotoxicity of xenobiotics Environ. Toxicol. Chem. 10:1991;1037-1043
6. Cooper E.L. Phylogeny of leukocytes. earthworm coelocytes in vitro and in vivo Lindahl-Kiessling K., Osoba D. Lymphocyte Recognition and Effector Mechanisms. Proceedings of the Eighth Leukocyte Culture Conference. 1974;155-162 Academic Press, New York
7. Cooper E.L., Cossarizza A., Suzuki M.M. Autogenic but not allogenic earthworm effector coelomocytes kill the mammalian tumor cell target K562. Cell. Immunol. 166:1995;113-122
8. Dean J.H., Murray M.J. Toxic responses of the immune system. Klaassen C.D., Amdur M.O., Doull J. Toxicology: The Basic Science of Poisons. Vol. 4:1990;282-333 McMillan, New York
9. Diogène J., Dufour M., Poirier G.G., Nadeau D. Extrusion of earthworm coelomocytes. comparison of the cell populations recovered from the species Lumbricus terrestris, Eisenia fetida and Octolasion tyrtaeum Lab. Anim. 31:1997;326-336
10. Eyambe G., Goven A.J., Fitzpatrick L.C., Venables B., Cooper E.L. Extrusion protocol for use in chronic immunotoxicity studies with earthworms (Lumbricus terrestris) coelomic leukocytes. Lab. Anim. 25:1991;61-67
11. Fitzpatrick L.C., Sassani R., Venables B.J., Goven A.J. Comparative toxicity of polychlorinated biphenyls to earthworms Eisenia fetida and Lumbricus terrestris. Environ. Pollut. 77:1992;65-69
12. Fordsmand-Scott J.J., Week J.M. Biomarkers in earthworms. Rev. Environ. Contam. Toxicol. 165:2000;117-159
13. Fournier M., Cyr D., Blakley B., Boermans H., Brousseau P. Phagocytosis as a biomarker of immunotoxicity in wildlife species exposed to environmental xenobiotics. Am. Zool. 40:2000;412-420
14. Fournier M., Cyr D., Brousseau P., Tryphonas H. Biomarkers in immunotoxicology. evolutionary perspective Guillette L., Crain D. Environmental Endocrine Disruptors. 2000;Taylor and Francis Publishers, New York
15. Fugere N., Brousseau P., Coderre D., Krzystyniak K., Fournier M. Heavy metal-specific inhibition of phagocytosis and different in vitro sensitivity of heterogeneous coelomocytes from Lumbricius terrestris. Toxicology. 109:1996;157-166
16. Giggleman M.A., Fitzpatrick L.C., Goven A.J., Venables B.J. Effects of pentachlorophenol on survival of earthworm (Lumbricus terrestris) and phagocytosis by their immuno active coelomocytes. Environ. Toxicol. Chem. 17:1998;2341-2394
17. Goven A.J., Venables B.J., Fitzpatrick L.C., Cooper E.L. An invertebrate model for analyzing effects of environmental xenobiotics on immunity. Clin. Ecol. 4:1988;150-154
18. Goven A.J., Eyambe G.S., Fitzpatrick L.C., Venables B.J., Cooper E.L. Cellular biomarkers for measuring toxicity of xenobiotics. effects of polychlorinated biphenyls on earthworm Lumbricus terrestris coelomocytes Environ. Toxicol. Chem. 12:1993;863-870
19. Goven A.J., Eyambe G.S., Fitzaptrick L.C., Venables B.J. Lysosyme activity in the earthworms (Lumbricus terrestris) coelomic fluid and coelomocytes. enzyme assay for immunotoxicity of xenobiotics Environ. Toxicol. Chem. 13:1994;607-613
20. Jandal Sigma Stat for Windows Version 1.0, 1992. MicroHelp, Inc. and Heiler Software Gmbh.
21. Koller L.D., Exon J.H. The rat as a model for immunotoxicity assessment. Dean H., Luster M., Munson A.E. Immunotoxicology and Immunopharmacology. 1985;99-111 Raven Press, New York
22. Rodriguez-Grau J., Venables B.J., Fitzpatrick L.C., Goven A.J. Suppression of secretory rosette formation by PCBs in Lumbricus terrestris. an earthworm assay for humoral immunotoxicity of xenobotics Environ. Toxicol. Chem. 8:1989;1201-1207
23. Sauvé S., Hendawi M., Brousseau P., Fournier M. Phagocytosis response of terrestrial and aquatic invertebrates following in vitro exposure to trace elements. Ecotoxicol. Environ. Saf. 52:2002;21-29
24. Toupin J., Marks D.H., Cooper E.L., Lamoureux G. Earthworms coelomocytes. In Vitro. 13:1977;218-222
25. Valembois P., Rouch P., Du Pasquier L. Dégradation in vitro de protéines étrangéres par les macrophages du lombricien Eisenia foetida (Sav.). C.R. Séances Acad. Sci. III (Série D). 277:1973;57-65
26. Ville P., Roch P., Cooper E.L., Masson P., Narbonne J. PCBs increase molecular-related activities (lysozyme, antibacterial, hemolysis, proteases) but inhibit macrophage-related functions (phagocytosis, wound healing) in the earthworms. J. Invert. Pathol. 65:1995;217-224
27. Ville P., Roch P., Cooper E.L., Narbonne J. Immune-modulator effects of carbaryl and 2,4-D in the earthworms Eisenia fetida andrei. Arch. Environ. Contam. Toxicol. 32:1997;291-297
28. Weeks J.M., Svendsen C. Neutral red retention by lysosomes from earthworm (Lumbricus rubells) coelomocytes. a simple biomarker of exposure to soil copper Environ. Toxicol. Chem. 15:1996;1801-1805