An optimized assay for transcription factor NF-κB with dsDNA-coupled microplate

Colloids and Surfaces B: Biointerfaces

Volumn 55, Issue 1, 2007, Pages 31-37

  Li, Jian lin ^a,b   Chen, Hong mei ^c   Li, Min li ^a   Hua, Dong ^a   Lu, Zu hong ^a   Wang, Jin ke ^a  

^a SOUTHEAST UNIVERSITY   (China)

^b HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^c CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Assay conditions; dsDNA coupled plate (dcPlate); NF B; Optimized parameters

Indexed keywords

ANTIBODIES; BINDING ENERGY; COLORIMETRIC ANALYSIS; COST EFFECTIVENESS; DNA; OPTIMIZATION; PROTEINS;

ASSAY CONDITIONS; DSDNA-COUPLED PLATE (DCPLATE); OPTIMIZED PARAMETERS;

BIOASSAY;

DNA BINDING PROTEIN; DOUBLE STRANDED DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PROTEIN ANTIBODY; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; ANALYTICAL PARAMETERS; ANTIGEN BINDING; ARTICLE; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; COLORIMETRY; DILUTION; DNA PROBE; GEL MOBILITY SHIFT ASSAY; IMMOBILIZATION; INCUBATION TIME; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; TEMPERATURE;

COLORIMETRY; DNA; HUMANS; NF-KAPPA B P50 SUBUNIT; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; RECOMBINANT PROTEINS; SURFACE PROPERTIES;

EID: 33846841010     PISSN: 09277765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.colsurfb.2006.11.015     Document Type: Article

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