Cloning, sequencing, and expression of a novel epoxide hydrolase gene from Rhodococcus opacus in Escherichia coli and characterization of enzyme

Applied Microbiology and Biotechnology

Volumn 74, Issue 1, 2007, Pages 99-106

  Liu, Zhiqiang ^a   Li, Yin ^b   Xu, Yingying ^b   Ping, Lifeng ^c   Zheng, Yuguo ^a  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

^b NORTH DAKOTA STATE UNIVERSITY   (United States)

^c INSTITUTE OF PLANT PROTECTION AND MICROBIOLOGY   (China)

Author keywords

Characterization; Cloning; Epoxide hydrolase; Prokaryotic expression; Rhodococcus opacus ML 0004

Indexed keywords

EPOXIDE HYDROLASE; PROKARYOTIC EXPRESSION; RHODOCOCCUS OPACUS ML-0004;

AMINO ACIDS; CLONING; ESCHERICHIA COLI; GENETIC ENGINEERING; MOLECULAR BIOLOGY; PLANTS (BOTANY);

GENES;

BARIUM; CALCIUM; COPPER; EDETATE SODIUM; EPOXIDE HYDROLASE; FUMARIC ACID; IRON; MERCURY; NICKEL; RECOMBINANT ENZYME; RECOMBINANT EPOXIDE HYDROLASE; SILVER; TARTARIC ACID; UNCLASSIFIED DRUG; ZINC;

AMINO ACID; CLONE; COLIFORM BACTERIUM; ENZYME; ENZYME ACTIVITY; GENE; GENE EXPRESSION; ORGANIC ACID; PLASMID;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; EPOXIDE HYDROLASE GENE; ESCHERICHIA COLI; GENE; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; RHODOCOCCUS; RHODOCOCCUS OPACUS; STEREOSPECIFICITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; EPOXIDE HYDROLASES; ESCHERICHIA COLI; KINETICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RHODOCOCCUS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; PROKARYOTA; RHODOCOCCUS OPACUS;

EID: 33846580455     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0635-8     Document Type: Article

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