Affinity purification and characterization of a yeast epoxide hydrolase

Biotechnology Letters

Volumn 21, Issue 6, 1999, Pages 511-517

  Botes, A L ^a  

^a UNIVERSITY OF THE FREE STATE   (South Africa)

Author keywords

Epoxide hydrolase; Purification; Rhodosporidium toruloides; Yeast

Indexed keywords

CARBOHYDRATE; EPOXIDE HYDROLASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID COMPOSITION; ARTICLE; CARBOHYDRATE ANALYSIS; ENZYME ACTIVITY; ENZYME PURIFICATION; MOLECULAR WEIGHT; YEAST;

RHODOSPORIDIUM TORULOIDES;

EID: 0032768820     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005500407152     Document Type: Article

References (21)

1. Archelas A (1998) Fungal epoxide hydrolases: new tools for the synthesisof enantiopure epoxidesand diols. J. Mol. Catalysis B: Enzymatic 5: 79-85.
2. Archelas A, Furstoss R (1998) Epoxide hydrolases: new tools for the synthesis of fine organic chemicals. TIBTECH 16: 108-116.
3. Blèe E, Schuber F (1992) Occurrence of fatty acid epoxide hydrolases in soy bean (Glycine max). Biochem. J. 282: 711-714.
4. Botes AL, Weijers CA, Van Dyk MS (1998) Biocatalytic resolution of 1,2-epoxyoctane using resting cells of different yeast strains with novel epoxide hydrolase activities. Biotechnol. Lett. 20: 421-426.
5. Cleij M, Archelas A, Furstoss R (1998) Microbiological Transformations - Part 42 - A 2-liquid-phase preparative-scale process for an epoxide hydrolase catalyzed resolution of para-bromoal pha-methyl styrene oxide - occurrence of a surprising enantioselectivity enhancement. Tetrahedron: Asymmetry 9: 1839-1842.
6. Dong MW, Grant JR (1985) High-speed liquid chromatographic analysis of amino acids by post-column sodium hypochlorite-ophthalaldehyde reaction. J. Chromatogr. 327: 17-25.
7. Jacobs MHJ, van den Wijngaard AJ, Pentenga M, Janssen DB (1991) Characterization of the epoxide hydrolase from an epichlorohydrin degrading Pseudomonas sp. Eur. J. Biochem. 202: 1217-1222.
8. Käppeli O, Sauer M, Fiechter A (1982) Convenient procedure for the isolation of highly enriched, cytochrome P-450 containing microsomal fraction from Candida tropicalis. Anal. Biochem. 126: 179-182.
9. Kroutil W, Genzel Y, Pietzsch M, Syldatk C, Faber K (1998) Purification and characterization of a highly selective epoxide hydrolase from Nocardia sp. Eh1. J. Biotechnol. 61: 143-150.
10. Lu AYH, Thomas PE, Ryan D, Jerina DM, Levin W(1979) Purification of human liver microsomal epoxide hydrase. J. Biol. Chem. 254: 5878-5881.
11. Misawa E, Chion CK, Archer IV, Woodland MP, Zhou NY, Carter SF, Widdowson DA, Leak DJ (1998) Characterization of a catabolic epoxide hydrolase from a Corynebacterium sp. Eur. J. Biochem. 253: 173-183.
12. Mischitz M, Faber K, Willets AJ (1995) Isolation of a highly enantioselective epoxide hydrolase from Rhodococcus sp. NCIMB 11216. Biotechnol. Lett. 17: 893-898.
13. Morisseau C, Nellaiah H, Archelas A, Furstoss R, Baratti JC (1997) Asymmetric hydrolysis of racemic para-nitrostyrene oxide using an epoxide hydrolase preparation from Aspergillus niger. Enzyme Microbiol. Technol. 20: 446-452.
14. Porter TD, Beck TW, Kasper CB (1986) Complementary DNA and amino acid sequence of rat liver microsomal, xenobiotic epoxide hydrolase. Arch. Biochem. Biophys. 248: 121-129.
15. Rink R, Fennema M, Smids M, Dehmel U, Janssen DB (1997) Primary structure and catalytic mechanism of the epoxide hydrolase from Agrobacterium radiobacter Ad1. J. Biol. Chem. 272: 14650-14657.
16. Robertso EF, Dannelly HK, Molloy PJ, Reeves HC (1987) Rapid isoelectric focussing in a vertical polyacrylamide minigel system. Anal. Biochem. 167: 290-294.
17. Wackett LP, Gibson DT (1982) Metabolism of xenobiotic compounds by enzymes in cell free extracts of the fungus Cunninghamella elegans. Biochem. J. 205: 117-122.
18. Weijers CAGM (1997) Enantioselective hydrolysisof aryl, alicyclic and aliphatic epoxides by Rhodotorula glutinia. Tetrahedron: Asymmetry 8: 639-647.
19. Weijers CAGM, de Bont JAM (1999) Epoxide hydrolases from yeasts and other sources versatile tools in biocatalysis. J. Mol. Catalysis B: Enzymatic 6: 199-214.
20. Wixtrom RN, Silva MH, Hammock BD (1988) Affinity purification of cytosolic epoxide hydrolase using derivatized epoxy-activated sepharose gels. Anal. Biochem. 169: 71-80.
21. Wojtasek H, Prestwich GD (1996) An insect juvenile hormone specific epoxide hydrolase is related to vertebrate microsomal epoxide hydrolases. Biochem. Biophys. Res. Commun. 220: 323-329.