Thymus uncoupling protein 1 is exclusive to typical brown adipocytes and is not found in thymocytes

Journal of Histochemistry and Cytochemistry

Volumn 55, Issue 2, 2007, Pages 183-189

  Frontini, Andrea ^a   Rousset, Sophie ^b   Cassard Doulcier, Anne Marie ^b   Zingaretti, Cristina ^a   Ricquier, Daniel ^b   Cinti, Saverio ^a  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b CNRS   (France)

Author keywords

Brown adipose tissue; Cold exposure; Immunohistochemistry; Rat; Thymus; UCP1

Indexed keywords

CELL MARKER; UNCOUPLING PROTEIN 1;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BROWN ADIPOSE TISSUE; COLD EXPOSURE; CONTROLLED STUDY; ENERGY EXPENDITURE; FATTY ACID OXIDATION; FEMALE; IMMUNOHISTOCHEMISTRY; INTESTINE; MALE; MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SKELETAL MUSCLE; SPLEEN; STOMACH; THERMOGENESIS; THYMOCYTE; UTERUS;

ADIPOCYTES; ADIPOSE TISSUE, BROWN; ANIMALS; ANIMALS, NEWBORN; FEMALE; IMMUNOHISTOCHEMISTRY; ION CHANNELS; LYMPHOCYTES; MALE; MICE; MICE, INBRED C57BL; MICROSCOPY, CONFOCAL; MITOCHONDRIAL PROTEINS; RATS; RATS, WISTAR; THYMUS GLAND;

ANIMALIA; MUS; RATTUS;

EID: 33846462100     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.6A7013.2006     Document Type: Article

References (35)

1. Aronson SM, Schwartzman G (1956) The histopathology of brown fat in experimental poliomyelitis. Am J Pathol 32:315-333
2. Arsenijevic D, Onuma H, Pecqueur C, Raimbault S, Manning BS, Miroux B, Couplan E, et al. (2000) Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production. Nat Genet 26:435-439
3. Bouillaud F, Ricquier D, Thibault J, Weissenbach J (1985) Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein. Proc Natl Acad Sci USA 82:445-448
4. Boyer BB, Kozak LP (1991) The mitochondrial uncoupling protein gene in brown fat: correlation between DNase I hypersensitivity and expression in transgenic mice. Mol Cell Biol 11:4147-4156
5. Cancello R, Zingaretti MC, Sarzani R, Ricquier D, Cinti S (1998) Leptin and UCP1 genes are reciprocally regulated in brown adipose tissue. Endocrinology 39:4747-4750
6. Cannon B, Hedin A, Nedergaard J (1982) Exclusive occurrence of thermogenin antigen in brown adipose tissue. FEBS Lett 150: 129-132
7. Cannon B, Nedergaard J (1985) The biochemistry of an inefficient tissue: brown adipose tissue. Essays Biochem 20:110-164
8. Carroll AM, Haines LR, Pearson TW, Brennan C, Breen EP, Porter RK (2004) Immunodetection of UCP1 in rat thymocytes. Biochem Soc Trans 32:1066-1067
9. Carroll AM, Haines LR, Pearson TW, Fallon PG, Walsh CM, Brennan CM, Breen EP, et al. (2005) Identification of a functioning mitochondrial uncoupling protein 1 in thymus. J Biol Chem 280:15534-15543
10. Cassard-Doulcier AM, Gelly C, Fox N, Schrementi J, Raimbault S, Klaus S, Forest C, et al. (1993) Tissue-specific and beta-adrenergic regulation of the mitochondrial uncoupling protein gene: control by cis-acting elements in the 5′-flanking region. Mol Endocrinol 7:497-506
11. Cinti S (1999) Adipose tissues and obesity. Ital J Anat Embryol 104:37-51
12. Cinti S (2001) The adipose organ: morphological perspectives of adipose tissues. Proc Nutr Soc 60:319-328
13. Cinti S (2005) The adipose organ. Prostaglandins Leukot Essent Fatty Acids 73:9-15
14. Cinti S, Cancello R, Zingaretti MC, Ceresi E, De Matteis R, Giordano A, Himms-Hagen J, et al. (2002) CL316,243 and cold stress induce heterogeneous expression of UCP1 mRNA and protein in rodent brown adipocytes. J Histochem Cytochem 50:21-31
15. Commins SP, Watson PM, Padgett MA, Dudley A, Argyropoulos G, Gettys TW (1999) Induction of uncoupling protein expression in brown and white adipose tissue by leptin. Endocrinology 140:292-300
16. Cousin B, Cinti S, Morroni M, Raimbault S, Ricquier D, Penicaud L, Casteilla L (1992) Occurrence of brown adipocytes in rat white adipose tissue: molecular and morphological characterization. J Cell Sci 103:931-942
17. De Matteis R, Ricquier D, Cinti S (1998) TH-, NPY-, SP-, and CGRP-immunoreactive nerves in interscapular brown adipose tissue of adult rats acclimated at different temperatures: an immunohistochemical study. J Neurocytol 27:877-886
18. Enerback S, Jacobsson A, Simpson EM, Guerra C, Yamashita H, Harper ME, Kozak LP (1997) Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese. Nature 387:90-94
19. Garruti G, Ricquier D (1992) Analysis of uncoupling protein and its mRNA in adipose tissue deposits of adult humans. Int J Obes Relat Metab Disord 16:383-390
20. Giordano A, Morroni M, Santone G, Marchesi GF, Cinti S (1996) Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation. J Neurocytol 25:125-136
21. Himms-Hagen J, Melnyk A, Zingaretti MC, Ceresi E, Barbatelli G, Cinti S (2000) Multilocular fat cells in WAT of CL-316243-treated rats derive directly from white adipocytes. Am J Physiol Cell Physiol 279:C670-681
22. Himms-Hagen J, Ricquier D (1998) Brown adipose tissue. In Bray GA, Bouchard C, James WPT, eds. Handbook of Obesity. New York, Marcel Dekker, 415-441
23. Jacobsson A, Stadler U, Glotzer MA, Kozak LP (1985) Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression. J Biol Chem 260: 16250-16254
24. Krauss S, Zhang CY, Lowell BB (2002) A significant portion of mitochondrial proton leak in intact thymocytes depends on expression of UCP2. Proc Natl Acad Sci USA 99:118-122
25. Lean ME, Branch WJ, James WP, Jennings G, Ashwell M (1983) Measurement of rat brown-adipose-tissue mitochondrial uncoupling protein by radioimmunoassay: increased concentration after cold acclimation. Biosci Rep 3:61-71
26. Murano I, Morroni M, Zingaretti MC, Oliver P, Sanchez J, Fuster A, Pico C, et al. (2005) Morphology of ferret subcutaneous adipose tissue after 6-month daily supplementation with oral beta-carotene. Biochim Biophys Acta 1740:305-312
27. Nagase I, Yoshida T, Kumamoto K, Umekawa T, Sakane N, Nikami H, Kawada T, et al. (1996) Expression of uncoupling protein in skeletal muscle and white fat of obese mice treated with thermogenic β3-adrenergic agonist. J Clin Invest 97:2898-2904
28. Nedergaard J, Golozoubova V, Matthias A, Asadi A, Jacobsson A, Cannon B (2001) UCP1: the only protein able to mediate adaptive non-shivering thermogenesis and metabolic inefficiency. Biochim Biophys Acta 1504:82-106
29. Negre-Salvayre A, Hirtz C, Carrera G, Cazenave R, Troly M, Salvayre R, Penicaud L, et al. (1997) A role for uncoupling protein-2 as a regulator of mitochondrial hydrogen peroxide generation. FASEB J 11:809-815
30. Nibbelink M, Moulin K, Arnaud E, Duval C, Penicaud L, Casteilla L (2001) Brown fat UCP1 is specifically expressed in uterine longitudinal smooth muscle cells. J Biol Chem 276:47291-47295
31. Nicholls DG, Locke RM (1984) Thermogenic mechanisms in brown fat. Physiol Rev 64:1-64
32. Ricquier D, Barlet JP, Garel JM, Combes-George M, Dubois MP (1983) An immunological study of the uncoupling protein of brown adipose tissue mitochondria. Biochem J 210:859-866
33. Ricquier D, Raimbault S, Champigny O, Miroux B, Bouillaud F (1991) The uncoupling protein is not expressed in rat liver. FEBS Lett 293:173-174
34. Roussel S, Alves-Guerra MC, Ouadghiri-Bencherif S, Kozak LP, Miroux B, Richard D, Bouillaud F, et al. (2003) Uncoupling protein 2, but not uncoupling protein 1, is expressed in the female mouse reproductive tract. J Biol Chem 278:45843-45847
35. 3-Adrenergic agonist induces a functionally active uncoupling protein in fat and slow-twitch muscle fibers. Am J Physiol 274:E469-475