Mice lacking mitochondrial uncoupling protein are cold-sensitive but not obese

Nature

Volumn 387, Issue 6628, 1997, Pages 90-94

  Enerbäck, Sven ^a,b   Jacobsson, Anders ^a,d   Simpson, Elizabeth M ^a   Guerra, Carmen ^a   Yamashita, Hitoshi ^a,e   Harper, Mary Ellen ^c   Kozak, Leslie P ^a  

^a JACKSON LABORATORY   (United States)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

^c UNIVERSITY OF OTTAWA   (Canada)

^d STOCKHOLM UNIVERSITY   (Sweden)

^e NATIONAL DEFENSE MEDICAL COLLEGE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

UNCOUPLING PROTEIN;

ANIMAL TISSUE; ARTICLE; BROWN ADIPOSE TISSUE; ENERGY BALANCE; MITOCHONDRIAL MEMBRANE; MOUSE; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY;

EID: 1842409029     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/387090a0     Document Type: Article

References (31)

1. Nicholls, D. G. & Locke, R.M. Thermogenic mechanism in brown fat. Physiol. Rev. 64, 1-64 (1984).
2. Foster, D. O. & Frydman, M. L. Tissue distribution of cold-induced thermogenesis in conscious warm-or cold-acclimated rats reevaluated from changes in tissue blood flow: the dominant role of brown adipose tissue in the replacement of shivering by nonshivering thermogenesis. Can. J. Physiol. Pharmacol. 57, 257-270 (1979).
3. Rothwell, N. J. & Stock, M. J. A role for brown adipose tissue in diet-induced thermogenesis. Nature 281, 31-35 (1979).
4. Champigny, O. et al. β3-Adrenergic receptor stimualtion restores message and expression of brown-fat mitochondrial uncoupling protein in adult dogs. Proc. Natl Acad. Sci. USA 88, 10774-10777 (1991).
5. Himms-Hagen, J. et al. Effect of CL-316,243, a thermogenic β3-agonist, on energy balance and brown and white adipose tissues in rat. Am. J. Physiol. 266, R1371-R1382 (1994).
6. Kozak, L. P., Kozak, U. C. & Clarke, G. T. Abnormal brown and white fat development in transgenic mice overexpressing glycerol 3-phosphate dehydrogenase. Genes Dev. 5, 2256-2264 (1991).
7. Lowell, B. B. et al. Development of obesity in transgenic mice after genetic ablation of brown adipose tissue. Nature 366, 740-742 (1993).
8. Kopecky, J., Clarke, G., Enerback, S., Spiegelman, B. & Kozak, L. P. Expression of the mitochondrial uncoupling protein gene from the aP2 promoter prevents genetic obesity. J. Clin. Invest. 96, 2914-2923 (1995).
9. Cummings, D. E. et al. Genetically lean mice result from targeted disruption of protein kinase A. Nature 382, 622-626 (1996).
10. Thomas, K. R. & Capecchi, M. R. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51, 503-512 (1987).
11. Gossler, A., Doestchman, T. C., Korn, R., Serfling, E. & Kemler, R. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc. Natl Acad. Sci. USA 83, 9065-9069 (1986).
12. Kozak, L. P., Britton, J. H., Kozak, U. C. & Wells, J. M. The mitochondrial uncoupling protein gene. Correlation of exon structure to transmembrane domains. J. Biol. Chem. 263, 12274-12277 (1988).
13. Cannon, B. & Vogel, G. The mitochondrial ATPase of brown adipose tissue. Purification and comparison with mitochondria ATPase from beef heart. FEBS Lett. 76, 284-289 (1977).
14. 3-adrenergic receptor gene. J. Biol. Chem. 270, 29483-29492 (1995).
15. Holloway, B. R. et al. ICI D7114 a novel selective β-adrenoceptor agonist selectively stimulates brown fat and increases whole body oxygen consumption. Br. J. Pharmacol. 104, 97-104 (1991).
16. Hamann, A., Flier, J. S. & Lowell, B. B. Decreased brown fat markedly enhances susceptibility to diet-induced obesity, diabetes and hyperlipidemia. Endocrinology 137, 21-29 (1996).
17. Billington, C. J., Briggs, J. E., Grace, M. & Levine, A. S. Effects of intracerebroventricular injection of neuropeptide Yon energy metabolism. Am. J. Physiol. 266, R1765-R1770 (1991).
18. Koza, R. A. et al. Sequence and tissue-dependent RNA expression of mouse FAD-linked glycerol-3-phosphate dehydrogenase. Arch. Biochem. Biophys. 336, 97-104 (1996).
19. Melnyk, A., Harper, M.-E. & Himms-Hagen, J. Raising at thermoneutrality prevents obesity and hyperphagia in brown adipose tissue-ablated transgenic mice. Am. J. Physiol. 272, R1088-R1093 (1997).
20. Leibel, R. L., Rosenbaum, M. & Hirsch, J. Changes in energy expenditure resulting from altered body weight. N. Engl. J. Med. 332, 621-628 (1995).
21. Lean, M. E. J. & James, W. P. T. in Brown Adipose Tissue (eds Trayhurn, P. & Nicholls, D. G.) 339-365 (Arnold, London, 1986).
22. Soriano, P., Montgomery, C., Geske, R. & Bradley, A. Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell 64, 693-702 (1991).
23. Chomczynski, P. & Sacchi, N. Single-step method of RNA isolation by acid guanidium thiocyanatephenol-chloroform extraction. Analyt. Biochem. 162, 156-159 (1987).
24. Derman, E. et al. Transcriptional control in the production of liver-specific mRNAs. Cell 23, 731-739 (1981).
25. Feinberg, A. P. & Vogelstein, B. A technique for radiolabelling DNA reflection endonuclease fragments to high specific activity. Analyt. Biochem. 132, 6-13 (1983).
26. Zeviani, M. et al. Sequence of cDNAs encoding subunit Vb of human and bovine cytochrome c oxidase. Gene 65, 1-11 (1988).
27. Bernlohr, D. A., Angus, C. W., Lane, M. D., Bolanowski, M. A. & Kelly, T. J. J. Expression of specific mRNAs during adipose differentiation: identification of an mRNA encoding a homologue of myelin P2 protein. Proc. Natl Acad. Sci. USA 81, 5468-5472 (1984).
28. Kozak, L. P. & Birkenmeier, E. H. Mouse sn-glycerol-3 phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence. Proc. Natl Sci. USA 80, 3020-3024 (1983).
29. Kyte, J. & Doolittle, R. F. A simple method for displaying the hydropathic character of a protein. J. Mol Biol. 157, 105-132 (1982).
30. Ma, S. W. & Foster, D. O. Starvation-induced changes in metabolic rate, Mood flow, and regional energy expenditure in rats. Can. J. Physiol. Pharmacol. 64, 1252-1258 (1986).
31. Thomas, S. A. & Palmiter, R. D. Thermoregulatory and metabolic phenotypes of mice lacking noradrenaline and adrenaline Nature 387, 94-97 (1997).