Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein-Barr virus replication and the simple tetracycline repressor

FEBS Journal

Volumn 274, Issue 3, 2007, Pages 783-790

  Bach, Markus ^a   Grigat, Silke ^a   Pawlik, Barbara ^a   Fork, Christian ^a   Utermöhlen, Olaf ^a   Pal, Sonia ^a   Banczyk, David ^a   Lazar, Andreas ^a   Schömig, Edgar ^a   Gründemann, Dirk ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Doxycycline; Epstein Barr virus; Polyadenylation; Regulated protein expression; Tetracycline repressor

Indexed keywords

COMPLEMENTARY DNA; DOXYCYCLINE; GREEN FLUORESCENT PROTEIN; HERPESVIRUS VECTOR; MESSENGER RNA; PLASMID VECTOR; RECOMBINASE; TETRACYCLINE;

ARTICLE; BINDING AFFINITY; CELL LINE; CELL MEMBRANE; CELLULAR DISTRIBUTION; CHIMERA; COMPARATIVE STUDY; CONTROLLED STUDY; EMBRYO; EPISOME; EPSTEIN BARR VIRUS; FLOW CYTOMETRY; FLUORESCENCE; GENE CASSETTE; GENETIC TRANSFECTION; GENOME; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PLASMID; POLYADENYLATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION; REPRESSOR GENE; VIRAL GENE DELIVERY SYSTEM; VIRUS REPLICATION;

BLOTTING, NORTHERN; CELL LINE; DOXYCYCLINE; FLOW CYTOMETRY; GENETIC VECTORS; HERPESVIRUS 4, HUMAN; HUMANS; PLASMIDS; PROTEIN BIOSYNTHESIS; REPLICATION ORIGIN; REPRESSOR PROTEINS; TETRACYCLINE; TRANSCRIPTION, GENETIC; TRANSFECTION; VIRUS REPLICATION;

CYTOMEGALOVIRUS; EUKARYOTA; HUMAN HERPESVIRUS 4;

EID: 33846446395     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2006.05623.x     Document Type: Article

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