Deletion of the glucose-6-phosphate dehydrogenase gene KlZWF1 affects both fermentative and respiratory metabolism in Kluyveromyces lactis

Eukaryotic Cell

Volumn 6, Issue 1, 2007, Pages 19-27

  Saliola, Michele ^a   Scappucci, Gina ^a   De Maria, Ilaria ^a   Lodi, Tiziana ^b   Mancini, Patrizia ^a   Falcone, Claudio ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF PARMA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; GLUCOSE 6 PHOSPHATE DEHYDROGENASE;

ARTICLE; BIOLOGICAL MODEL; BREATHING; FERMENTATION; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; KLUYVEROMYCES; METABOLISM; NUCLEOTIDE SEQUENCE; OXIDATION REDUCTION REACTION; OXIDATIVE STRESS;

BASE SEQUENCE; CARBON; FERMENTATION; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSEPHOSPHATE DEHYDROGENASE; KLUYVEROMYCES; MODELS, BIOLOGICAL; OXIDATION-REDUCTION; OXIDATIVE STRESS; RESPIRATION; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

KLUYVEROMYCES; KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 33846441674     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00189-06     Document Type: Article

References (39)

1. Aguilera, A. 1986. Deletion of the phosphoglucose isomerase structural gene makes growth and sporulation glucose dependent in Saccharomyces cerevisiae. Mol. Gen. Genet. 204:310-316.
2. Au, S. W., C. E. Naylor, S. Gover, L. Vandeputte-Rutten, D. A. Scopes, P. J. Mason, L. Luzzatto, V. M. Lam, and M. J. Adams. 1999. Solution of the structure of tetrameric human glucose 6-phosphate dehydrogenase by molecular replacement. Acta Crystallogr. D 55:826-834.
3. Au, S. W., S. Gover, V. M. Lam, and M. J. Adams. 2000. Human glucose-6-phosphate dehydrogenase: the crystal structure reveals a structural NADP+ molecule and provides insights into enzyme deficiency. Structure 8:293-303.
4. Bakker, B. M., K. M. Overkamp, A. J. van Maris, P. Kotter, M. A. Luttik, J. P. van Dijken, and J. T. Pronk. 2001. Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae. FEMS Microbiol. Rev. 25:15-37.
5. Becker, D. M., and L. Guarente. 1991. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194:182-187.
6. Beutler, E. 1994. G6PD deficiency. Blood 84:3613-3636.
7. Boles, E., W. Lehnert, and F. K. Zimmermann. 1993. The role of the NAD-dependent glutamate dehydrogenase in restoring growth on glucose of a Saccharomyces cerevisiae phosphoglucose isomerase mutant. Eur. J. Biochem. 217:469-477.
8. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254.
9. Chiu, D. T. Y., J. M. Ou, S. W. Huang, and K. J. Tsai. 1996. The functional role of the eight cysteines in human glucose-6-phosphate dehydrogenase (G6PD). Blood 88:1213-1221.
10. Ferrero, I., A. M. Viola, and A. Goffeau. 1981. Induction by glucose of an antimycin-insensitive, azide-sensitive respiration in the yeast Kluyveromyces lactis. Antonie Leeuwenhoek 47:11-24.
11. Goffrini, P., A. A. Algeri, C. Donnini, M. Wésolowski-Louvel, and I. Ferrero. 1989. RAG1 and RAG2: nuclear genes involved in the dependence/independence on mitochondrial respiratory function for growth on sugar. Yeast 5:99-105.
12. Goffrini, P., M. Wésolowski-Louvel, and I. Ferrero. 1991. A phosphoglucose isomerase gene is involved in the Rag phenotype of the yeast Kluyveromyces lactis. Mol. Gen. Genet. 228:401-409.
13. Gonzalez Siso, M. I., M. A. Freire Picos, and M. E. Cerdan. 1996. Reoxidation of the NADPH produced by the pentose phosphate pathway is necessary for the utilization of glucose by Kluyveromyces lactis rag2 mutants. FEBS Lett. 387:7-10.
14. Grabowska, D., and A. Chelstowska. 2003. The ALD6 gene product is indispensable for providing NADPH in yeast cells lacking glucose-6-phosphate dehydrogenase activity. J. Biol. Chem. 278:13984-13988.
15. Izawa, S., K. Maeda, T. Miki, Y. Inoue, and A. Kimura. 1998. Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae. Biochem. J. 330:811-817.
16. Jacoby, J., C. P. Hollenberg, and J. J. Heinisch. 1993. Transaldolase mutants in the yeast Kluyveromyces lactis provide evidence that glucose can be metabolized through the pentose phosphate pathway. Mol. Microbiol. 10:867-876.
17. Juhnke, H., B. Krems, P. Kotter, and K. D. Entian. 1996. Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative stress. Mol. Gen. Genet. 252:456-464.
18. Kletzien, R. F., P. K. W. Harris, and L. A. Foellmi. 1994. Glucose-6-phosphate dehydrogenase: a "housekeeping" enzyme subject to tissue-specific regulation by hormones, nutrients, and oxidant stress. FASEB J. 8:174-181.
19. Lodi, T., and I. Ferrero. 1993. Isolation of the DLD1 gene of Saccharomyces cerevisiae encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase. Mol. Gen. Genet. 238:315-324.
20. Lutstorf, U., and R. Megnet. 1968. Multiple forms of alcohol dehydrogenase in Saccharomyces cerevisiae. Arch. Biochem. Biophys. 126:933-944.
21. Madeo, F., E. Frohlich, M. Ligr, M. Grey, S. Sigrist, D. H. Wolf, and K.-U. Frohlich. 1999. Oxygen stress: a regulator of apoptosis in yeast. J. Cell Biol. 145:757-767.
22. Mazzoni, C., M. Saliola, and C. Falcone. 1992. Ethanol-induced and glucose-insensitive alcohol dehydrogenase in the yeast Kluyveromyces lactis. Mol. Microbiol. 6:2279-2286.
23. Minard, K. I., and L. McAlister-Henn. 2005. Sources of NADPH in yeast vary with carbon source. J. Biol. Chem. 280:39890-39896.
24. Nogae, I., and M. Johnston. 1990. Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase. Gene 96:161-169.
25. Notaro, R., A. Afolayan, and L. Luzzatto. 2000. Human mutations in glucose-6-phosphate dehydrogenase reflect evolutionary history. FASEB J. 14:485-494.
26. Overkamp, K. M., B. M. Bakker, H. Y. Steensma, J. P. van Dijken, and J. T. Pronk. 2002. Two mechanisms for oxidation of cytosolic NADPH by Kluyveromyces lactis mitochondria. Yeast 19:813-824.
27. Rigoulet, M., H. Aguilaniu, N. Avéret, O. Bunoust, N. Camougrand, X. Grandier-Vazeille, C. Larsson, I. L. Pahlman, S. Manon, and L. Gustafsson. 2004. Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae. Mol. Cell. Biochem. 256/257:73-81.
28. Rothstein, R. 1983. One step gene disruption in yeast. Methods Enzymol. 101:202-211.
29. Rowland, P., A. K. Basak, S. Gover, H. R. Levy, and M. J. Adams. 1994. The three dimensional structure of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A. Structure 2:1073-1087.
30. Saliola, M., and C. Falcone. 1995. Two mitochondrial alcohol dehydrogenase activities of Kluyveromyces lactis are differentially expressed during respiration and fermentation. Mol. Gen. Genet. 249:665-672.
31. Saliola, M., P. C. Bartoccioni, I. De Maria, T. Lodi, and C. Falcone. 2004. The deletion of the succinate dehydrogenase gene KlSDH1, in Kluyveromyces lactis, does not lead to respiratory deficiency. Eukaryot. Cell 3:589-597.
32. Schulz, J. B., M. Weller, and T. Klockgether. 1996. Potassium deprivation-induced apoptosis of cerebellar granule neurons: a sequential requirement for new mRNA and protein synthesis, ICE-like protease activity, and reactive oxygen species. J. Neurosci. 16:4696-4706.
33. Scopes, D., J. M. Bautista, C. E. Naylor, M. J. Adams, and P. J. Mason. 1998. Amino acid substitutions at the dimer interphase of human glucose-6-phosphate dehydrogenase that increase thermostability and reduce the stabilising effect of NADP. Eur. J. Biochem. 251:382-388.
34. Steensma, H. Y., and J. J. M. Ter Linde. 2001. Plasmids with the Cre recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis. Yeast 18:469-472.
35. Tarrio, N., S. Diaz Prado, M. E. Cerdan, and M. I. Gonzalez Siso. 2005. The nuclear genes encoding the internal (KlNDI1) and external (KlNDE1) alternative NAD(P)H:ubiquinone oxidoreductases of mitochondria from Kluyveromyces lactis. Biochim. Biophys. Acta 1707:199-210.
36. Tarrio, N., M. Becerra, M. E. Cerdan, and M. I. Gonzalez Siso. 2006. Reoxidation of cytosolic NADPH in Kluyveromyces lactis. FEMS Yeast Res. 6:371-380.
37. Thomas, D., H. Cherest, and Y. Surdin-Kerian. 1991. Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur. EMBO J. 10:547-553.
38. Wach, A., A. Brachat, R. Pohlmann, and P. Philippsen. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10:1793-1808.
39. Wenderoth, I., R. Scheibe, and A. von Schaewen. 1997. Identification of the cysteine residues involved in redox modification of plant plastidic glucose-6-phosphate dehydrogenase. J. Biol. Chem. 272:26985- 26990.