Plasmids with the Cre-recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis

Yeast

Volumn 18, Issue 5, 2001, Pages 469-472

  Steensma, H Y ^a,b,c   Ter Linde, J J M ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^c LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Cre lox; Dominant marker; Kluyveromyces lactis; NatMX; Plasmid

Indexed keywords

CELL MARKER; CLONNAT; CRE RECOMBINASE; DNA; NAT MARKER; NOURSEOTHRICIN; UNCLASSIFIED DRUG; URA 3 PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CENTROMERE; CONTROLLED STUDY; DNA REPLICATION ORIGIN; GENE CASSETTE; GENE MUTATION; GENE REPLICATION; KLUYVEROMYCES; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE;

ACETYLTRANSFERASES; GENETIC MARKERS; INTEGRASES; KLUYVEROMYCES; PLASMIDS; SACCHAROMYCES CEREVISIAE; VIRAL PROTEINS;

KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 0035970512     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.696     Document Type: Article

References (11)

1. Exploring redundancy in the yeast genome: An improved strategy for use of the cre/loxP system
2. Applications of high efficiency lithium acetate transformation of intact yeast cells using single-stranded nucleic acids as carrier
3. A phosphoglucose isomerase gene is involved in the Rag phenotype of the yeast Kluyveromyces lactis
4. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae
5. A new efficient gene disruption cassette for repeated use in budding yeast
6. High efficiency transformation of Escherichia coli with plasmids
7. A strong carbon source effect is mediated by pUC plasmid sequences in a series of classical yeast vectors designed for promoter characterization
8. Expression cassettes for formaldehyde and fluoroacetate resistance, two dominant markers in Saccharomyces cerevisiae
9. The lysine-rich C-terminal repeats of the centromere-binding factor 5 (Cbf5) of Kluyveromyces lactis are not essential for function
10. Cheap and simple yeast media