Nuclear repositioning of the VSG promoter during developmental silencing in Trypanosoma brucei

Journal of Cell Biology

Volumn 176, Issue 2, 2007, Pages 133-139

  Landeira, David ^a   Navarro, Miguel ^a  

^a INSTITUTO DE PARASITOLOGÍA Y BIOMEDICINA LÓPEZ NEYRA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEAR PROTEIN; PROTEIN PROCYCLIN; RIBOSOME DNA; RNA POLYMERASE II; UNCLASSIFIED DRUG; VARIANT SURFACE GLYCOPROTEIN;

ARTICLE; CELL NUCLEUS MEMBRANE; CHROMATIN CONDENSATION; CHROMOSOME REARRANGEMENT; CONTROLLED STUDY; GENE SILENCING; INTERPHASE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN LOCALIZATION; TRANSCRIPTION REGULATION; TRYPANOSOMA BRUCEI;

AMANITINS; ANIMALS; CELL DIFFERENTIATION; CELL LINE; CELL NUCLEOLUS; CELL NUCLEUS; CHROMATIN; CHROMOSOME POSITIONING; DEOXYURACIL NUCLEOTIDES; DNA, RIBOSOMAL; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE SILENCING; GREEN FLUORESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; NUCLEAR ENVELOPE; PROMOTER REGIONS (GENETICS); PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS; RNA POLYMERASE I; TRANSCRIPTION, GENETIC; TRYPANOSOMA BRUCEI BRUCEI; VARIANT SURFACE GLYCOPROTEINS, TRYPANOSOMA;

TRYPANOSOMA BRUCEI;

EID: 33846406103     PISSN: 00219525     EISSN: 00219525     Source Type: Journal    
DOI: 10.1083/jcb.200607174     Document Type: Article

References (29)

1. Acosta-Serrano, A., R.N. Cole, A. Mehlert, M.G. Lee, M.A. Ferguson, and P.T. Englund. 1999. The procyclin repertoire of Trypanosoma brucei. Identification and structural characterization of the Glu-Pro-rich polypeptides. J. Biol. Chem. 274:29763-29771.
2. Alibu, V.P., L. Storm, S. Haile, C. Clayton, and D. Horn. 2005. A doubly inducible system for RNA interference and rapid RNAi plasmid construction in Trypanosoma brucei. Mol. Biochem. Parasitol. 139:75-82.
3. Barry, J.D., and R. McCulloch. 2001. Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite. Adv. Parasitol. 49:1-70.
4. Berberof, M., L. Vanhamme, P. Tebabi, A. Pays, D. Jefferies, S. Welburn, and E. Pays. 1995. The 3′-terminal region of the mRNAs for VSG and procyclin can confer stage specificity to gene expression in Trypanosoma brucei. EMBO J. 14:2925-2934.
5. Borst, P. 2002. Antigenic variation and allelic exclusion. Cell. 109:5-8.
6. Chaves, I., J. Zomerdijk, A. Dirks-Mulder, R.W. Dirks, A.K. Raap, and P. Borst. 1998. Subnuclear localization of the active variant surface glycoprotein gene expression site in Trypanosoma brucei. Proc. Natl. Acad. Sci. USA. 95:12328-12333.
7. Chung, H.M., M.G.-S. Lee, and L.H.T. Van der Ploeg. 1992. RNA polymerase I-mediated protein-coding gene expression in Trypanosoma brucei. Parasitol. Today. 8:414-418.
8. Conway, C., R. McCulloch, M.L. Ginger, N.P. Robinson, A. Browitt, and J.D. Barry. 2002. Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes. J. Biol. Chem. 277:21269-21277.
9. Cross, G.A.M., L.E. Wirtz, and M. Navarro. 1998. Regulation of vsg expression site transcription and switching in Trypanosoma brucei. Mol. Biochem. Parasitol. 91:77-91.
10. Dietzel, S., K. Zolghadr, C. Hepperger, and A.S. Belmont. 2004. Differential large-scale chromatin compaction and intranuclear positioning of transcribed versus non-transcribed transgene arrays containing beta-globin regulatory sequences. J. Cell Sci. 117:4603-4614.
11. Engstler, M., and M. Boshart. 2004. Cold shock and regulation of surface protein trafficking convey sensitization to inducers of stage differentiation in Trypanosoma brucei. Genes Dev. 18:2798-2811.
12. Gartenberg, M.R., F.R. Neumann, T. Laroche, M. Blaszczyk, and S.M. Gasser. 2004. Sir-mediated repression can occur independently of chromosomal and subnuclear contexts. Cell. 119:955-967.
13. Gasser, S.M. 2002. Visualizing chromatin dynamics in interphase nuclei. Science. 296:1412-1416.
14. Horn, D., and G.A.M. Cross. 1995. A developmentally regulated position effect at a telomeric locus in Trypanosoma brucei. Cell. 83:555-561.
15. Hotz, H.R., S. Biebinger, J. Flaspohler, and C. Clayton. 1998. PARP gene expression: control at many levels. Mol. Biochem. Parasitol. 91:131-143.
16. Janzen, C.J., F. Lander, O. Dreesen, and G.A. Cross. 2004. Telomere length regulation and transcriptional silencing in KU80-deficient Trypanosoma brucei. Nucleic Acids Res. 32:6575-6584.
17. Janzen, C.J., S.B. Hake, J.E. Lowell, and G.A. Cross. 2006. Selective di- or trimethylation of histone H3 lysine 76 by two DOT1 homologs is important for cell cycle regulation in Trypanosoma brucei. Mol. Cell. 23:497-507.
18. Navarro, M., and K. Gull. 2001. A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei. Nature. 414:759-763.
19. Navarro, M., G.A. Cross, and E. Wirtz. 1999. Trypanosoma brucei variant surface glycoprotein regulation involves coupled activation/inactivation and chromatin remodeling of expression sites. EMBO J. 18:2265-2272.
20. Overath, P., J. Czichos, and C. Haas. 1986. The effect of citrate/cis-aconitate on oxidative metabolism during transformation of Trypanosoma brucei. Eur. J. Biochem. 160:175-182.
21. Pays, E., L. Vanhamme, and D. Perez-Morga. 2004. Antigenic variation in Trypanosoma brucei: facts, challenges and mysteries. Curr. Opin. Microbiol. 7:369-374.
22. Robinett, C.C., A. Straight, G. Li, C. Willhelm, G. Sudlow, A. Murray, and A.S. Belmont. 1996. In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J. Cell Biol. 135:1685-1700.
23. Roditi, I., H. Schwarz, T.W. Pearson, R.P. Beecroft, M.K. Liu, R.O. Williams, and P. Overath. 1989. Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei. J. Cell Biol. 108:737-746.
24. Rudenko, G., S. Le Blancq, J. Smith, M.G. Lee, A. Rattray, and L.H. Van der Ploeg. 1990. Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei. Mol. Cell. Biol. 10:3492-3504.
25. Rudenko, G., H.-M. Chung, V.P. Pham, and L.H. Van der Ploeg. 1991. RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei. EMBO J. 10:3387-3397.
26. Scheer, U., and R. Hock. 1999. Structure and function of the nucleolus. Curr. Opin. Cell Biol. 11:385-390.
27. Spector, D.L. 2003. The dynamics of chromosome organization and gene regulation. Annu. Rev. Biochem. 72:573-608.
28. Straight, A.F., A.S. Belmont, C.C. Robinett, and A.W. Murray. 1996. GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion. Curr. Biol. 6:1599-1608.
29. Wirtz, E., S. Leal, C. Ochatt, and G.A.M. Cross. 1999. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99:89-101.