Functional immobilization of the small GTPase Rab6A on DNA-gold nanoparticles by using a site-specifically attached poly(ethylene glycol) linker and thiol place-exchange reaction

ChemBioChem

Volumn 8, Issue 1, 2007, Pages 32-36

  Becker, Christian F W ^a   Marsac, Yoann ^a   Hazarika, Pompi ^b   Moser, Jens ^a   Goody, Roger S ^a   Niemeyer, Christof M ^b  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b TU DORTMUND UNIVERSITY   (Germany)

Author keywords

Gold; Nanotechnology; Native chemical ligation; PEG linker; Protein immobilization; Rab GTPase

Indexed keywords

EDETIC ACID; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; MACROGOL; NANOPARTICLE; OLIGONUCLEOTIDE; RAB PROTEIN; RAB6A PROTEIN; THIOGLYCOLIC ACID; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENDOPLASMIC RETICULUM; GOLGI COMPLEX; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN VARIANT; WESTERN BLOTTING;

DNA; ESCHERICHIA COLI; GOLD; GTP PHOSPHOHYDROLASES; GUANOSINE TRIPHOSPHATE; HUMANS; MODELS, CHEMICAL; NANOPARTICLES; POLYETHYLENE GLYCOLS; PROTEIN STRUCTURE, TERTIARY; RAB GTP-BINDING PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SULFHYDRYL COMPOUNDS; TIME FACTORS;

EID: 33846311444     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200600422     Document Type: Article

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