Transcriptional analysis of the toxigenic element of Clostridium difficile

Microbial Pathogenesis

Volumn 22, Issue 3, 1997, Pages 143-154

  Hammond, Georgia A ^a   Lyerly, David M ^a   Johnson, John L ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

Author keywords

bacterial toxins; Clostridium difficile; pseudomembranous colitis; toxigenic element; toxin A and toxin B; transcription

Indexed keywords

BACTERIAL TOXIN;

ARTICLE; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; DNA SEQUENCE; GENETIC TRANSCRIPTION; MOLECULAR HYBRIDIZATION; NONHUMAN; PRIORITY JOURNAL; PSEUDOMEMBRANOUS COLITIS; TOXIN ANALYSIS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM DIFFICILE;

EID: 0030933825     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.1996.0100     Document Type: Article

References (42)

1. Fluit AC, Wolfhagen MJHM, Verdonk GPHT, Jansze M, Torensma R, Verhoef J. Nontoxigenic strains ofClostridium difficile. J Clin Microbiol. 29:1991;2666-2667.
2. Price SB, Phelps CJ, Wilkins TD, Johnson JL. Cloning of the carbohydrate-binding portion of the toxin A gene ofClostridium difficile. Curr Microbiol. 16:1987;55-60.
3. Johnson JL, Dove CH, Price SB, Sickles TW, Phelps CJ, Wilkins TD. The toxin A gene ofClostridium difficile. Hardie JM, Borriello SP, eds. Anaerobes today. West Sussex: John Wiley & Sons. 1988;115-123.
4. Eichel-Streiber Cv, Suckau D, Wachter M, Hadding U. Cloning and characterization of overlapping DNA fragments of the toxin A gene ofClostridium difficile. J Gen Microbiol. 135:1989;55-64.
5. Dove CH, Wang S-Z, Price SB. Molecular characterization of theClostridium difficile. Infect Immun. 58:1990;480-488.
6. Sauerborn M, Eichel-Streiber Cv. Nucleotide sequence ofClostridium difficile. Nucleic Acids Res. 18:1990;1629-1630.
7. Barroso LA, Wang S-Z, Phelps CJ, Johnson JL, Wilkins TD. Nucleotide sequence of theClostridium difficile. Nucleic Acids Res. 18:1990;4004.
8. Eichel-Streiber Cv, Laufenberg-Feldmann R, Sartingen S, Schulze J, Sauerborn M. Cloning of theClostridium difficile. Med Microbiol Immuno. 179:1990;271-279.
9. Johnson JL, Phelps C, Barroso L, Roberts MD, Lyerly DM, Wilkins TD. Cloning and expression of the toxin B gene ofClostridium difficile. Curr Microbiol. 20:1990;397-401.
10. Hammond GA, Johnson JL. The toxigenic element ofClostridium difficile. Microb Pathog. 19:1995;203-213.
11. Eichel-Streiber Cv, Sauerborn M. Clostridium difficile. Gene. 96:1990;107-113.
12. Eichel-Streiber Cv. Molecular biology of theClostridium difficile. Sebald M, ed. Genetics and molecular biology of anaerobic bacteria. New York: Springer-Verlag. 1993;264-289.
13. Eichel-Streiber Cv, Laufenberg-Feldmann R, Sartingen S, Schulze J, Sauerborn M. Comparative sequence analysis of theClostridium difficile. Mol Gen Genet. 233:1992;260-268.
14. Torres JF. Purification and characterization of toxin B from a strain ofClostridium difficile. J Med Microbiol. 35:1991;40-44.
15. Borriello SP, Wren BW, Hyde S. Molecular, immunological and biological characterization of a toxin A negative, toxin B positive strain ofClostridium difficile. Infect Immun. 60:1992;4192-4199.
16. Lyerly DM, Barroso A, Wilkins TD, Depitre C, Corthier G. Characterization of a toxin A-negative, toxin B-positive strain ofClostridium difficile. Infect Immun. 60:1992;4633-4639.
17. Lyerly DM, Wilkins TD. Commercial latex test forClo-stridium difficile. J Clin Microbiol. 23:1986;622-623.
18. Wren B, Heard SR, Tabaqchali S. Association between production of toxins A and B and types ofClostridium difficile. J Clin Pathol. 40:1987;1397-1401.
19. Lyerly DM, Barroso LA, Wilkins TD. Identification of the latex test-reactive protein ofClostridium difficile. J Clin Microbiol. 29:1991;2639-2642.
20. Young M, Staudenbauer WL, Minton NP. Genetics ofClostridium. Minton NP, Clarke DJ, eds. Clostridia. New York: Plenum Press. 1989;63-103.
21. Chen KC-K, Chen J-S, Johnson JL. Structural features of multiplenifClostridium pasteurianum. J Bacteriol. 166:1986;162-172.
22. Perelle S, Gibert M, Boquet P, Popoff MR. Characterization ofClostridium perfringensEscherichia coli. Infect Immun. 61:1993;5147-5156.
23. Phelps CJ, Lyerly DM, Johnson JL, Wilkins TD. Construction and expression of the completeClostridium difficileEscherichia coli. Infect Immun. 59:1991;150-153.
24. Young M, Minton NP, Staudenbaeur WL. Recent advances in the genetics of clostridia. FEMS Microbiol Rev. 63:1989;301-326.
25. Graves MC, Rabinowitz JC. In vivo and in vitro transcription of theClostridium pasteurianum. J Biol Chem. 261:1986;11409-11415.
26. Frisby D, Zuber P. Analysis of the upstream activating sequence and site of carbon and nitrogen source repression in the promoter of an early-induced sporulation gene ofBacillus subtilis. J Bacteriol. 173:1991;7557-7564.
27. Ross W, Gosink KK, Salomon J. A third recognition element in bacterial promoters: DNA binding by the α subunit of RNA polymerase. Science. 262:1993;1407-1413.
28. Lyerly DM, Wilkins TD. Toxins of anaerobes. Levett PN, ed. Anaerobic microbiology: a practical approach. Oxford: IRL Press. 1991;163-181.
29. Holdeman, LV, Cato, EP, Moore, WEC, 1977, eds. Anaerobe laboratory manual. Blacksburg, VA: VPI Anaerobe Laboratory, Virginia Polytechnic Institute and State University.
30. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning. A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Press. 1989.
31. Puissant C, Houdebine L-M. An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Biotechniques. 8:1990;148-149.
32. Kroczek RA, Siebert E. Optimization of Northern analysis by vacuum-blotting, RNA-transfer visualization, and ultraviolet fixation. Anal Biochem. 184:1990;90-95.
33. Ahmad I, Finkelstein JA, Steggles AW. The analysis of RNA byin situ. Biotechniques. 8:1990;162-165.
34. Chaudhari N. Plasmid fragments as accurate markers for Northern blots. Biotechniques. 10:1991;601-605.
35. Ehtesham NZ, Hasnain SE. Direct in-gel hybridization, without blotting, using nick-translated cloned DNA probe. Biotechniques. 11:1991;718-721.
36. Saiki RK, Gelfand DH, Stoffel S. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:1988;487-491.
37. Marmur J. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol. 3:1961;209-218.
38. Johnson L. Similarity analysis of DNAs. Gerhardt P, Murray RGE, Wood WA, Krieg NR, eds. Methods for general and molecular bacteriology. Washing- ton, DC: American Society for Microbiology. 1994;655-682.
39. Alam J, Whitaker RA, Krogmann DW, Curtis SE. Isolation and sequence of the gene for ferrodoxin I from the cyanobacteriumAnabena. J Bacteriol. 168:1986;1265-1271.
40. Sanger F, Nickelen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 74:1977;5463-5467.
41. Lyerly DM, Sullivan NM, Wilkins TD. Enzyme-linked immunosorbent assay forClostridium difficile. J Clin Microbiol. 17:1983;72-78.
42. Ehrich M, Van Tassell RL, Libby JM, Wilkins TD. Production ofClostridium difficile. Infect Immun. 28:1980;1041-1043.