Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipids

EMBO Journal

Volumn 26, Issue 1, 2007, Pages 19-27

  Camberg, Jodi L ^a,d   Johnson, Tanya L ^b   Patrick, Marcella ^b   Abendroth, Jan ^c   Hol, Wim G J ^c   Sandkvist, Maria ^b  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Acidic phospholipids; ATPase; Protein secretion

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CARDIOLIPIN; EPSE PROTEIN; EPSL PROTEIN; PHOSPHOLIPID DERIVATIVE; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE; COMPLEX FORMATION; CYTOPLASM; ENZYME ACTIVITY; HYDROLYSIS; MUTAGENESIS; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; VIBRIO CHOLERAE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEINS; CARDIOLIPINS; CLONING, MOLECULAR; CROSS-LINKING REAGENTS; CYTOPLASM; DOSE-RESPONSE RELATIONSHIP, DRUG; HYDROLYSIS; MEMBRANE PROTEINS; MUTATION; PHOSPHOLIPIDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; VIBRIO CHOLERAE;

VIBRIO CHOLERAE;

EID: 33846190237     PISSN: 02614189     EISSN: 14602075     Source Type: Journal    
DOI: 10.1038/sj.emboj.7601481     Document Type: Article

References (25)

1. Abendroth J, Bagdasarian M, Sandkvist M, Hol WG (2004) The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily. J Mol Biol 344: 619-633
2. Abendroth J, Murphy P, Sandkvist M, Bagdasarian M, Hol WG (2005) The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio cholerae. J Mol Biol 348: 845-855
3. Auvray F, Ozin AJ, Claret L, Hughes C (2002) Intrinsic membrane targeting of the flagellar export ATPase FliI: interaction with acidic phospholipids and FliH. J Mol Biol 318: 941-950
4. Ball G, Chapon-Herve V, Bleves S, Michel G, Bally M (1999) Assembly of XcpR in the cytoplasmic membrane is required for extracellular protein secretion in Pseudomonas aeruginosa. J Bacteriol 181: 382-388
5. Camberg JL, Sandkvist M (2005) Molecular analysis of the Vibrio cholerae type II secretion ATPase EpsE. J Bacteriol 187: 249-256
6. Cianciotto NP (2005) Type II secretion: a protein secretion system for all seasons. Trends Microbiol 13: 581-588
7. Filloux A (2004) The underlying mechanisms of type II protein secretion. Biochim Biophys Acta 1694: 163-179
8. Herdendorf TJ, McCaslin DR, Forest KT (2002) Aquifex aeolicus PilT, homologue of a surface motility protein, is a thermostable oligomeric NTPase. J Bacteriol 184: 6465-6471
9. Johnson TL, Abendroth J, Hol WG, Sandkvist M (2006) Type II secretion: from structure to function. FEMS Microbiol Lett 255: 175-186
10. Kawai F, Shoda M, Harashima R, Sadaie Y, Hara H, Matsumoto K (2004) Cardiolipin domains in Bacillus subtilis marburg membranes. J Bacteriol 186: 1475-1483
11. Lill R, Dowhan W, Wickner W (1990) The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteins. Cell 60: 271-280
12. Michel G, Bleves S, Ball G, Lazdunski A, Filloux A (1998) Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosa. Microbiology 144 (Part 12): 3379-3386
13. Mileykovskaya E, Dowhan W (2000) Visualization of phospholipid domains in Escherichia coli by using the cardiolipin-specific fluorescent dye 10-N-nonyl acridine orange. J Bacteriol 182: 1172-1175
14. Mileykovskaya E, Dowhan W (2005) Role of membrane lipids in bacterial division-site selection. Curr Opin Microbiol 8: 135-142
15. Possot OM, Vignon G, Bomchil N, Ebel F, Pugsley AP (2000) Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE. J Bacteriol 182: 2142-2152
16. Powl AM, East JM, Lee AG (2005) Heterogeneity in the binding of lipid molecules to the surface of a membrane protein: hot spots for anionic lipids on the mechanosensitive channel of large conductance MscL and effects on conformation. Biochemistry 44: 5873-5883
17. Py B, Loiseau L, Barras F (2001) An inner membrane platform in the type II secretion machinery of Gram-negative bacteria. EMBO Rep 2: 244-248
18. Robien MA, Krumm BE, Sandkvist M, Hol WG (2003) Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae. J Mol Biol 333: 657-674
19. Sakai D, Horiuchi T, Komano T (2001) ATPase activity and multimer formation of Pilq protein are required for thin pilus biogenesis in plasmid R64. J Biol Chem 276: 17968-17975
20. Sandkvist M (2001) Biology of type II secretion. Mol Microbiol 40: 271-283
21. Sandkvist M, Bagdasarian M, Howard SP, DiRita VJ (1995) Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO J 14: 1664-1673
22. Sandkvist M, Hough LP, Bagdasarian MM, Bagdasarian M (1999) Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae. J Bacteriol 181: 3129-3135
23. Sandkvist M, Michel LO, Hough LP, Morales VM, Bagdasarian M, Koomey M, DiRita VJ, Bagdasarian M (1997) General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in Vibrio cholerae. J Bacteriol 179: 6994-7003
24. Savvides SN, Yeo HJ, Beck MR, Blaesing F, Lurz R, Lanka E, Buhrdorf R, Fischer W, Haas R, Waksman G (2003) VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion. EMBO J 22: 1969-1980
25. Shiue SJ, Kao KM, Leu WM, Chen LY, Chan NL, Hu NT (2006) XpsE oligomerization triggered by ATP binding, not hydrolysis, leads to its association with XpsL. EMBO J 25: 1426-1435