Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE

Journal of Bacteriology

Volumn 182, Issue 8, 2000, Pages 2142-2152

  Possot, Odile M ^a   Vignon, Guillaume ^a   Bomchil, Natalia ^a,b   Ebel, Frank ^a   Pugsley, Anthony P ^a  

^a CNRS   (France)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PULLULANASE;

ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; ERWINIA; IMMUNOPRECIPITATION; KLEBSIELLA OXYTOCA; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DEGRADATION; PROTEIN INTERACTION; PROTEIN SECRETION;

BACTERIAL PROTEINS; CARRIER PROTEINS; CELL MEMBRANE; CROSS-LINKING REAGENTS; ENZYME STABILITY; ERWINIA; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; GLYCOSIDE HYDROLASES; KLEBSIELLA; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MULTIGENE FAMILY; PLASMIDS; PROTEIN BINDING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CHRYSANTHEMI; ESCHERICHIA COLI; KLEBSIELLA OXYTOCA; NEGIBACTERIA; PECTOBACTERIUM CAROTOVORUM; PROKARYOTA;

EID: 0034034763     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.8.2142-2152.2000     Document Type: Article

References (45)

1. Ball, G., V. Chapon-Hervé, S. Bleves, G. Michel, and M. Bally. 1999. Assembly of XcpR in the cytoplasmic membrane is required for extracellular protein secretion in Pseudomonas aeruginosa. J. Bacteriol. 181:382-388.
2. Bleves, S., M. Gérard-Vincent, A. Lazdunski, and A. Filloux. 1999. Structure-function analysis of XcpP, a component involved in general secretory pathway-dependent protein secretion in Pseudomonas aeruginosa. J. Bacteriol. 181:4012-4019.
3. Blum, P., D. Holzschu, H. S. Kwan, D. Riggs, and S. Artz. 1989. Gene replacement and retrieval with recombinant M13mp bacteriophages. J. Bacteriol. 171:538-546.
4. Brockman, R. W., and L. A. Heppel. 1968. On the localization of alkaline phosphatase and cyclic phosphodiesterase in Escherichia coli. Biochemistry 7:2554-2562.
5. de Groot, A., J.-J. Krijger, A. Filloux, and J. Tommassen. 1996. Characterization of type II protein secretion (xcp) genes in the plant growth-stimulating Pseudomonas aeruginosa, strain WCS358. Mol. Gen. Genet. 250:491-504.
6. d'Enfert, C., and A. P. Pugsley. 1989. Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein required for pullulanase secretion. J. Bacteriol. 171:3673-3679.
7. d'Enfert, C., A. Ryter, and A. P. Pugsley. 1987. Cloning and expression in Escherichia coli of the Klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase. EMBO J. 6:3531-3538.
8. Guilvout, I., K. R. Hardie, N. Sauvonnet, and A. P. Pugsley. 1999. Genetic dissection of the outer membrane secretin PulD: are there distinct domains for multimerization and secretion specificity? J. Bacteriol. 181: 7212-7220.
9. Hardie, K. R., S. Lory, and A. P. Pugsley. 1996. Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein. EMBO J. 15:978-988.
10. Hardie, K. R., A. Schulze, M. W. Parker, and J. T. Buckley. 1995. Vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation. Mol. Microbiol. 17:1035-1044.
11. Hardie, K. R., A. Seydel, I. Guilvout, and A. P. Pugsley. 1996. The secretin-specific, chaperone-like protein of the general secretory pathway: separation of proteolytic protection and piloting functions. Mol. Microbiol. 22:967-976.
12. Hirst, T. R., and R. Leece. 1991. The phenomenon of toxin secretion by vibrios and aeromonads. Experientia 47:429-431.
13. Kagami, Y., M. Ratliff, M. Surber, A. Martinez, and D. Nunn. 1998. Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin-like component XcpT by the cytoplasmic component XcpR. Mol. Microbiol. 27:221-233.
14. Kornacker, M. G., and A. P. Pugsley. 1989. Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsiella pneumoniae UNF5023. Mol. Microbiol. 4:73-85.
15. Kornacker, M. G., B. Remsburg, and R. Menzel. 1998. Gene activation by the AraC protein can be inhibited by DNA looping between AraC and a LexA repressor that interacts with AraC: possible applications as a two-hybrid system. Mol. Microbiol. 30:615-624.
16. Kunkel, T. A., J. D. Roberts, and R. A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:367-382.
17. Lindeberg, M., G. P. C. Salmond, and A. Collmer. 1996. Complementation of deletion mutations in a cloned functional cluster of Erwinia chrysanthemi out genes with Erwinia carotovora out homologs reveals OutC and OutD as candidate gatekeepers of species-specific secretion of proteins via the type II pathway. Mol. Microbiol. 20:175-190.
18. Michel, G., S. Bleves, G. Ball, A. Lazdunski, and A. Filloux. 1998. Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosa. Microbiology 144:3379-3386.
19. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
20. Nouwen, N., N. Ranson, H. Saibil, B. Wolpensinger, A. Engel, A. Ghazi, and A. P. Pugsley. 1999. Secretin PulD: association with pilot protein PulS, structure and ion-conducting channel formation. Proc. Natl. Acad. Sci. USA 96:8173-8177.
21. Overbye, Michel, L., M. Sandkvist, and M. Bagdasarian. 1995. Specificity of the protein secretory apparatus: secretion of the heat-labile enterotoxin B subunit pentamers by different species of Gram-negative bacteria. Gene 152:41-45.
22. Possot, O., C. d'Enfert, I. Reyss, and A. P. Pugsley. 1992. Pullulanase secretion in Escherichia coli K12 requires a cytoplasmic protein and a putative polytopic cytoplasmic membrane protein. Mol. Microbiol. 6:95-105.
23. Possot, O., L. Letellier, and A. P. Pugsley. 1997. Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway. Mol. Microbiol. 24:457-464.
24. Possot, O., and A. Pugsley. 1997. The conserved tetracysteine motif in the general secretory pathway component PulE is required for efficient pullulanase secretion. Gene 192:45-50.
25. Possot, O., and A. P. Pugsley. 1994. Molecular characterization of PulE, a protein required for pullulanase secretion. Mol. Microbiol. 12:287-299.
26. Possot, O. M., M. Gérard, and A. P. Pugsley. 1999. Membrane association and multimerization of secreton component PulC. J. Bacteriol. 181:4004-4011.
27. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
28. Pugsley, A. P. 1996. Multimers of the precursor of a type IV pilin-like component of the general secretory pathway are unrelated to pili. Mol. Microbiol. 20:1235-1245.
29. Pugsley, A. P. 1993. Processing and methylation of PulG, a pilin-like component of the general secretory pathway of Klebsiella oxytoca. Mol. Microbiol. 9:295-308.
30. Pugsley, A. P., C. d'Enfert, I. Reyss, and M. G. Kornacker. 1990. Genetics of extracellular protein secretion by Gram-negative bacteria. Annu. Rev. Genet. 24:67-90.
31. Pugsley, A. P., and B. Dupuy. 1992. An enzyme with type IV prepilin peptidase activity is required to process a component of the general extracellular protein secretion pathway of Klebsiella oxytoca. Mol. Microbiol. 6:751-760.
32. Pugsley, A. P., and O. Possot. 1993. The general secretory pathway of Klebsiella oxytoca: no evidence for relocalization or assembly of pilin-like PulG protein into a multiprotein complex. Mol. Microbiol. 10:665-674.
33. Pugsley, A. P., and I. Reyss. 1990. Five genes at the 3′ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion. Mol. Microbiol. 4:365-379.
34. Py, B., L. Loiseau, and F. Barras. 1999. Assembly of the type II secretion machinery of Erwinia chrysanthemi: direct interaction and associated conformational change between OutE, the putative ATP-binding component and the membrane protein OutL. J. Mol. Biol. 289:659-670.
35. Reyss, I., and A. P. Pugsley. 1990. Five additional genes in the pulC-O operon of the Gram-negative bacterium Klebsiella oxytoca UNF5023 that are required for pullulanase secretion. Mol. Gen. Genet. 222:176-184.
36. Sandkvist, M., M. Bagdasarian, S. P. Howard, and V. J. DiRita. 1995. Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO J. 14:1664-1673.
37. Sandkvist, M., L. P. Hough, M. M. Bagdasarian, and M. Bagdasarian. 1999. Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae. J. Bacteriol. 181:3129-3135.
38. Sauvonnet, N., P. Gounon, and A. P. Pugsley. 2000. PpdD type IV pilin of Escherichia coli K-12 can be assembled into pili in Pseudomonas aeruginosa. J. Bacteriol. 182:848-854.
39. Sauvonnet, N., I. Poquet, and A. P. Pugsley. 1995. Extracellular secretion of pullulanase is unaffected by minor sequence changes but is usually prevented by adding reporter proteins to its N-or C-terminal end. J. Bacteriol. 177: 5238-5246.
40. Sauvonnet, N., and A. P. Pugsley. 1996. Identification of two regions of Klebsiella oxytoca pullulanase that together are capable of promoting β-lactamase secretion by the general secretory pathway. Mol. Microbiol. 22:1-7.
41. Shevchik, V. E., J. Robert-Badouy, and G. Condemine. 1997. Specific interaction between OutD, an Erwinia chrysanthemi outer membrane protein of the general secretory pathway, and secreted proteins. EMBO J. 16:3007-3016.
42. Spratt, B. G., P. J. Hedge, S. te Heesen, A. Edelman, and J. K. Broome-Smith. 1986. Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene 41:337-342.
43. Thomas, J. D., P. J. Reeves, and G. P. C. Salmond. 1997. The general secretion pathway of Erwinia carotovora subsp. carotovora: analysis of the membrane topology of OutC and OutF. Microbiology 143:713-720.
44. Treptow, N. A., and H. A. Shuman. 1985. Genetic evidence for substrate and periplasmic-binding-protein recognition by the MalF and MalG proteins, cytoplasmic membrane components of the Escherichia coli maltose transport system. J. Bacteriol. 163:654-660.
45. Turner, L. R., J. W. Olson, and S. Lory. 1997. The XcpR protein of Pseudomonas aeruginosa dimerizes via its N-terminus. Mol. Microbiol. 26:877-887.