Homogeneous time-resolved fluorescence assays for the detection of activity and inhibition of phosphatase enzymes employing phosphorescently labeled peptide substrates

Analytica Chimica Acta

Volumn 583, Issue 2, 2007, Pages 349-356

  O'Shea, Desmond J ^a   O'Riordan, Tomás C ^a   O'Sullivan, Paul J ^a   Papkovsky, Dmitri B ^a  

^a UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

Metalloporphyrin labels; Phosphorogenic phosphopeptide substrates; Protein tyrosine phosphatases; Time resolved fluorescence

Indexed keywords

CHYMOTRYPSIN; DEPHOSPHORYLATION; METALLOPORPHYRIN LABELS; PHOSPHOROGENIC PHOSPHOPEPTIDE SUBSTRATES; PROTEIN TYROSINE PHOSPHATASES; TIME RESOLVED FLUORESCENCE;

ANTIBODIES; BIOASSAY; CELLS; ENZYME INHIBITION; FLUORESCENCE;

ENZYME KINETICS;

[4 [4 (DIMETHYLAMINO)PHENYLAZO]BENZOIC ACID N SUCCINIMIDYL ESTER]; AMINE; ANTIBODY; AZO COMPOUND; BENZOIC ACID DERIVATIVE; CD45 ANTIGEN; CHYMOTRYPSIN; COPROPORPHYRIN; ESTER DERIVATIVE; MALEIMIDE; METHYL GROUP; PEPTIDE; PHENYL GROUP; PHOSPHATASE; PLATINUM COPROPORPHYRIN; PLATINUM DERIVATIVE; PROTEIN TYROSINE PHOSPHATASE; RECOMBINANT ANTIGEN; SUCCINIMIDE DERIVATIVE; UNCLASSIFIED DRUG; VANADATE SODIUM;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYST; CELL LYSATE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; METHODOLOGY; PHOSPHORESCENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DEPHOSPHORYLATION; PROTEIN FOLDING; TIME RESOLVED FLUORESCENCE;

ANIMALS; CATTLE; ENZYME ACTIVATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; JURKAT CELLS; LUMINESCENT AGENTS; LUMINESCENT MEASUREMENTS; PEPTIDES; PHOSPHORIC MONOESTER HYDROLASES; SUBSTRATE SPECIFICITY; TIME FACTORS;

EID: 33846141791     PISSN: 00032670     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aca.2006.10.022     Document Type: Article

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