Development of a time-resolved fluorescent assay for measuring tyrosine-phosphorylated proteins in cells

Analytical Biochemistry

Volumn 309, Issue 1, 2002, Pages 150-157

  Waddleton, Deena ^a   Ramachandran, Chidambaram ^a   Wang, Qingping ^a  

^a MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

A431 cells; DELFIA assay; Epidermal growth factor receptor; Insulin receptor; KRC 7 cells; Protein tyrosine phosphatases; Tyrosine phosphorylation

Indexed keywords

AMINO ACIDS; ANTIBODIES; CELLS; CYTOLOGY; FLUORESCENCE; INSULIN; PHOSPHATASES;

EPIDERMAL GROWTH FACTOR RECEPTORS; FLUORESCENT ASSAYS; IMMUNOBLOTTING; INSULIN RECEPTORS; PHOSPHORYLATED PROTEINS; PROTEIN-TYROSINE PHOSPHATASE; TYROSINE PHOSPHORYLATION; WELL-ESTABLISHED TECHNIQUES;

PHOSPHORYLATION;

EPIDERMAL GROWTH FACTOR RECEPTOR; INSULIN RECEPTOR; PROTEIN TYROSINE PHOSPHATASE; ENZYME INHIBITOR; EPIDERMAL GROWTH FACTOR; EUROPIUM; INSULIN; MONOCLONAL ANTIBODY; PHOSPHOTYROSINE; PROTEIN TYROSINE KINASE;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; COLORIMETRY; CONFORMATIONAL TRANSITION; ENZYME LINKED IMMUNOSORBENT ASSAY; GLYCOGEN SYNTHESIS; IMMUNE RESPONSE; INSULIN RESISTANCE; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; ANIMAL; BIOTINYLATION; CELL CULTURE; CHEMISTRY; COMPARATIVE STUDY; DOSE RESPONSE; DRUG ANTAGONISM; FLUOROIMMUNOASSAY; LIVER CELL CARCINOMA; METABOLISM; METHODOLOGY; PHOSPHORYLATION; RAT; SERODIAGNOSIS; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOTINYLATION; BLOTTING, WESTERN; CARCINOMA, HEPATOCELLULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; EPIDERMAL GROWTH FACTOR; EUROPIUM; FLUOROIMMUNOASSAY; INSULIN; PHOSPHORYLATION; PHOSPHOTYROSINE; PRECIPITIN TESTS; PROTEIN-TYROSINE KINASES; PROTEIN-TYROSINE-PHOSPHATASE; RATS; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECEPTOR, INSULIN; TUMOR CELLS, CULTURED;

EID: 0036808565     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00292-0     Document Type: Article

References (28)

1. T. Hunter, Protein kinases and phosphatases: The yin and yang of protein phosphorylation and signaling, Cell 80 (1995) 225-236.
2. A.R. Saltiel, J.E. Pessin, Insulin signaling pathways in time and space, Trends Cell Biol. 12 (2002) 65-71.
3. M.F. White, C.R. Kahn, The insulin signaling system, J. Biol. Chem. 269 (1994) 1-4.
4. M. Kasuga, F.A. Karlsson, C.R. Kahn, Insulin stimulates the phosphorylation of the 95,000 dalton subunit of its own receptor, Science 215 (1982) 185-187.
5. O.M. Rosen, R. Herrera, Y. Olowe, L.M. Petruzzeli, M.H. Cobb, Phosphorylation activates the insulin receptor protein kinase, Proc. Natl. Acad. Sci. USA 80 (1983) 3237-3240.
6. T.A. Gustafson, S.A. Moodie, B.E. Lavan, The insulin receptor and metabolic signaling, Rev. Physiol. Biochem. Pharmacol. 137 (1999) 71-190.
7. J.C. Byon, A.B. Kusari, J. Kusari, Protein tyrosine phosphatase-1B acts as a negative regulator of insulin signaling transduction, Mol. Cell. Biochem. 182 (1998) 101-108.
8. M. Elchebly, P. Payette, E. Michaliszyn, W. Cromlish, S. Collins, A.L. Loy, D. Normandin, A. Cheng, J. Himms-Hagen, C.C. Chan, C. Ramachandran, M.J. Gresser, M. Tremblay, B.P. Kennedy, Increased insulin sensitivity and obesity resistance in mice lacking the protein tyrosine phosphatase-1B gene, Science 283 (1999) 1544-1548.
9. B.P. Kennedy, C. Ramachandran, Protein tyrosine phosphatase-1B in diabetes, Biochem. Pharmacol. 60 (2000) 877-883.
10. L.D. Klamen, O. Boss, O.D. Peroni, J.K. Kim, J.L. Martino, J.M. Zabolotny, N. Moghal, M. Lubkin, Y.B. Kim, A.H. Sharpe, A. Stricker-Krongrad, G.I. Shulman, B.G. Neel, B.B. Kahn, Increased energy expenditure, decreased adiposity and tissue specific insulin sensitivity in protein tyrosine phosphatase 1B deficient mice, Mol. Cell. Biol. 20 (2000) 5479-5489.
11. F. Ahmad, J.L. Azevedo Jr., R. Cortright, G.L. Dohm, B.J. Goldstein, Alterations in skeletal muscle protein-tyrosine phosphatase activity and expression in insulin resistant human obesity and diabetes, J. Clin. Invest. 100 (1997) 449-458.
12. I. Hemmila, Time resolved fluorometry-advantages and potentials in high throughput screening assays, in: J. Delvin (Ed.), High Throughput Screening, Marcel Dekker, New York, 1997, pp. 361-376.
13. H.E. Tornqvist, J. Avruch, Relationship of site-specific β subunit tyrosine autophosphorylation to insulin activation of the insulin receptor protein kinase activity, J. Biol. Chem. 263 (1988) 4593-4601.
14. J. Krutzfeldt, A. Grunweller, W. Raasch, M. Drenckhan, H.H. Klein, Microtiter well assays for protein tyrosine phosphatase activities directed against phosphorytated insulin receptor or insulin receptor substrate-1, Anal. Biochem. 271 (1999) 97-99.
15. Y. Okada, M. Yoshida, S. Baba, K. Shii, Development of vanadate sensitive human erythrocytes insulin receptor tyrosine phosphatase assay, Diabetes Res. Clin. Practice 41 (1998) 157-163.
16. Y. Yoshimasa, J.I. Paul, J. Whittaker, D.F. Steiner, Effects of amino acid replacements within the tetrabasic cleavage site expressed in Chinese hamster ovary cells, J. Biol. Chem. 265 (1990) 17230-17237.
17. J.W. Koontz, M. Iwahashi, Insulin as a potent, specific growth factor in a rat hepatoma cell line, Science 211 (1981) 947-949.
18. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
19. G. Huyer, S. Liu, J. Kelley, J. Moffat, P. Payette, B. Kennedy, G. Tsaprailis, M.J. Gresser, C. Ramachandran, Mechanism of protein-tyrosine phosphatases by vanadate and pervanadate, J. Biol. Chem. 272 (1997) 843-851.
20. S.-O. Mikalsen, O. Kaalhus, Properties of pervanadate and permolybdate. Connexin43, phosphatase inhibition and thiol reactivity as model systems, J. Biol. Chem. 273 (1998) 10036-10045.
21. N. Osherov, A. Levitzki, Epidermal growth factor dependent activation of src-family kinases, Eur. J. Biochem. 225 (1994) 1047-1053.
22. M. Kovalenko, A. Gazit, A. Bohmer, C. Rorsman, L. Ronnstrand, C.-H. Heldin, J. Waltenberger, F.-D. Bohmer, A. Levitzki, Selective platelet-derived growth factor receptor kinase blockers reverse sis-transformation, Cancer Res. 54 (1994) 6106-6114.
23. T. Akiyama, J. Ishida, S. Nakagawa, H. Ogaware, S. Watanabe, N. Itoh, M. Shibuya, Y. Fukami, Genistein, a specific inhibitor of tyrosine-specific protein kinases, J. Biol. Chem. 262 (1987) 5592-5595.
24. B.L. Seely, P.A. Staubs, D.R. Reichart, P. Berhanu, K.L. Milarski, A.R. Saltiel, J. Kusare, J.M. Olefsky, Protein tyrosine phosphatase 1B interacts with the activated insulin receptor, Diabetes 45 (1996) 1379-1385.
25. D. Bandyopadhyay, A. Kusari, K.A. Kenner, F. Liu, J. Chernoff, T.A. Gustafson, J. Kusari, Protein tyrosine phosphatase 1B complexes with the insulin receptor in vivo and is tyrosine-phosphorylated in the presence of insulin, J. Biol. Chem. 272 (1997) 1639-1645.
26. F. Ahmad, P.-M. Li, J. Meyerovitch, B.J. Goldstein, Osmotic loading of neutralizing antibodies demonstrates a role for protein-tyrosine phosphatase 1B in negative regulation of the insulin action pathway, J. Biol. Chem. 270 (1995) 20503-20508.
27. D.E. Biazzo-Ashnault, Y.-W. Park, R.T. Cummings, V. Ding, D.E. Moller, B.B. Ahang, S.A. Quershi, Detection of insulin receptor tyrosine kinase activity using time-resolved fluorescence energy transfer technology, Anal. Biochem. 291 (2001) 155-158.
28. Y. Amir-Zaltsman, O. Mazor, B. Gayer, A. Scherz, Y. Salomon, F. Kohen, Inhibitors of protein tyrosine phosphorylation: Preliminary assessment of activity by time-resolved fluorescence, Luminescence 15 (2000) 377-380.