Preparation and characterization of recombinant protein ScFv(CD11c)-TRP2 for tumor therapy from inclusion bodies in Escherichia coli

Protein Expression and Purification

Volumn 52, Issue 1, 2007, Pages 131-138

  Kou, Geng ^a,b   Shi, Shu ^a   Wang, Hao ^a,b   Tan, Min ^b   Xue, Jingya ^b   Zhang, Dapeng ^a   Hou, Sheng ^a,b   Qian, Weizhu ^a   Wang, Shuhui ^a   Dai, Jianxin ^a   Li, Bohua ^a,b   Guo, Yajun ^a,b  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

^b Shanghai Center for Cell Engineering and Antibody   (China)

Author keywords

Dendritic cell; Expression; Purification; Recombinant protein; Refolding

Indexed keywords

ANTINEOPLASTIC AGENT; GLYCOPROTEIN P 15095; LEUKOCYTE ANTIGEN; MEMBRANE PROTEIN; PEPTIDE FRAGMENT; PEPTIDE SVYDFFVWL; PRIMER DNA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CHEMISTRY; DENDRITIC CELL; DRUG EFFECT; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOUSE; NEOPLASM; NUCLEOTIDE SEQUENCE; PROTEIN FOLDING; RESTRICTION MAPPING;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD11C; ANTINEOPLASTIC AGENTS; BASE SEQUENCE; CLONING, MOLECULAR; DENDRITIC CELLS; DNA PRIMERS; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MEMBRANE PROTEINS; MICE; NEOPLASMS; PEPTIDE FRAGMENTS; PROTEIN FOLDING; RECOMBINANT PROTEINS; RESTRICTION MAPPING;

CRICETINAE; ESCHERICHIA COLI; MURINAE;

EID: 33845996221     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.08.007     Document Type: Article

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