Chromatographic purification of an insoluble histidine tag recombinant Ykt6p SNARE from Arabidopsis thaliana over-expressed in E. coli

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 808, Issue 1, 2004, Pages 83-89

  Vincent, Patrick ^a   Dieryck, Wilfrid ^a,b   Maneta Peyret, Lilly ^a   Moreau, Patrick ^a   Cassagne, Claude ^a,b   Santarelli, Xavier ^b  

^a University Victor Segalen   (France)

^b UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

coat proteins; COP; endoplasmic reticulum; ER; EST; expressed sequence tag; GA; Golgi apparatus; His tag; histidine tag; IMAC; immobilized metal affinity chromatography; LB; Lennox broth; PET; reverse transcription polymerase chain reaction; RT PCR

Indexed keywords

CELL IMMOBILIZATION; CELLS; ESCHERICHIA COLI; LIPIDS; PROTEINS; PURIFICATION; SOLUBILITY; YEAST;

ENDOPLASMIC RETICULUM (ER); GOLGI APPARATUS (GA); HISTIDINES;

CHROMATOGRAPHY;

ARABIDOPSIS PROTEIN; RECOMBINANT PROTEIN; SNARE PROTEIN;

ARABIDOPSIS; CELL DISRUPTION; CENTRIFUGATION; CONFERENCE PAPER; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ESCHERICHIA COLI; EXPRESSED SEQUENCE TAG; GOLGI COMPLEX; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LIPID TRANSPORT; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTIDINE; RECOMBINANT PROTEINS;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 3242752805     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2004.03.028     Document Type: Conference Paper

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