Prokaryotic expression, refolding, and purification of fragment 450-650 of the spike protein of SARS-coronavirus

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 169-174

  Zhao, Jin Cun ^a   Zhao, Zhen Dong ^a   Wang, Wei ^a   Gao, Xiao Ming ^a  

^a PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

On column refolding; SARS coronavirus; Spike protein

Indexed keywords

FREUND ADJUVANT; HISTIDINE; MEMBRANE PROTEIN; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BAGG ALBINO MOUSE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; IMMUNIZATION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; MOUSE; PROTEIN FOLDING; SARS CORONAVIRUS; SUBCUTANEOUS DRUG ADMINISTRATION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; FREUND'S ADJUVANT; GENETIC VECTORS; HISTIDINE; HUMANS; IMMUNIZATION; IMMUNIZATION, SECONDARY; INJECTIONS, SUBCUTANEOUS; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED BALB C; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PROTEIN FOLDING; RECOMBINANT PROTEINS; SARS VIRUS; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); CORONAVIRUS; ESCHERICHIA COLI; PROKARYOTA; SARS ASSOCIATED CORONAVIRUS; SARS CORONAVIRUS; SARS COV;

EID: 11844288309     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.10.004     Document Type: Article

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