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Since the reactivation process can be viewed as a reduction, H oxinact, ne- → Hox, we can express it in terms of the Nernst equation: E, E°, 2.3RT/nF) log Q, where R, molar gas constant, T is the absolute temperature 283 K for all experiments, F is the Faraday constant, and n is the number of electrons involved in the reduction process. We will assume Q, Hox, Hox inact, Positive current, i, is a direct measure of H 2 oxidation activity; thus, the amount of oxidized active enzyme, Hox, can be stated as [Hox] ∝ i. The maximum reactivation current, imax, gives a measure of the total amount of enzyme adsorbed on the electrode; thus, the amount of inactive enzyme, Hoxinact, can be stated as [Hox inact] ∝
-
max - i)/i] vs E therefore has a gradient of nF/2.3RT.
-
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49
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33845927065
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2 oxidation activity from CO, with the gradients of film loss changing by no more than a factor of 2.
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2 oxidation activity from CO, with the gradients of film loss changing by no more than a factor of 2.
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