Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry

Applied and Environmental Microbiology

Volumn 72, Issue 12, 2006, Pages 7739-7747

  Dinoto, Achmad ^a   Marques, Tatiana M ^a   Sakamoto, Kanta ^a   Fukiya, Satoru ^a   Watanabe, Jun ^a   Ito, Susumu ^a   Yokota, Atsushi ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; DYES; FLUORESCENCE; POPULATION STATISTICS;

BIFIDOBACTERIUM SPECIES; FLUORESCEIN ISOTHIOCYANATE (FITC); FLUORESCENCE IN SITU HYBRIDIZATION-FLOW CYTOMETRY (FISH-FCM); POPULATION DYNAMICS;

BACTERIA;

CARBOCYANINE; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; INDODICARBOCYANINE; PREBIOTIC AGENT; RAFFINOSE;

BACTERIUM; BIOMONITORING; FECES; FEEDING; FLOW CYTOMETRY; FLUORESCENCE; HYBRIDIZATION; POPULATION DYNAMICS;

ADULT; ARTICLE; AUTOFLUORESCENCE; BACTERIAL COUNT; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANGULATUM; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM CATENULATUM; BIFIDOBACTERIUM DENTIUM; BIFIDOBACTERIUM LONGUM; FEASIBILITY STUDY; FECES; FECES ANALYSIS; FEMALE; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN EXPERIMENT; MALE; NORMAL HUMAN; POPULATION DYNAMICS;

ADULT; BIFIDOBACTERIUM; FECES; FEMALE; FLOW CYTOMETRY; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; MIDDLE AGED; RAFFINOSE; SPECIES SPECIFICITY;

BIFIDOBACTERIUM; BIFIDOBACTERIUM ANGULATUM; BIFIDOBACTERIUM BIFIDUM; BIFIDOBACTERIUM BREVE; BIFIDOBACTERIUM CATENULATUM; BIFIDOBACTERIUM DENTIUM; BIFIDOBACTERIUM LONGUM;

EID: 33845542334     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01777-06     Document Type: Article

References (30)

1. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow eytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
2. Aubin, J. E. 1979. Autofluorescence of viable cultured mammalian cells. J. Histochem. Cytochem. 27:36-43.
3. Bartosch, S., E. J. Woodmansey, J. C. M. Paterson, M. E. T. McMurdo, and G. T. Macfarlane. 2005. Microbiological effects of consuming a synbiotic containing Bifidobacterium bifidum, Bifidobacterium lactis, and oligofructose in elderly persons, determined by real-time polymerase chain reaction and counting of viable bacteria. Clin. Infect. Dis. 40:28-37.
4. Ben-Amor, K., H. Heilig, H. Smidt, E. E. Vaughan, T. Abee, and W. M. de Vos. 2005. Genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16S rRNA gene analysis. Appl. Environ. Microbiol. 71:4679-4689.
5. Benno, Y., K. Endo, N. Shiragami, K. Sayama, and T. Mitsuoka. 1987. Effects of raffinose intake on human fecal microflora. Bifidobacteria Microflora 6:59-63.
6. Benson, R. C., R. A. Meyer, M. E. Zaruba, and G. M. McKhann. 1979. Cellular autofluorescence-is it due to flavins? J. Histochem. Cytochem. 27:44-48.
7. Cole, J. R., B. Chai, R. J. Farris, Q. Wang, S. A. Kulam, D. M. McGarrell, G. M. Garrity, and J. M. Tiedje. 2005. The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res. 33:D294-D296.
8. Dinoto, A., A. Suksomcheep, S. Ishizuka, H. Kimura, S. Hanada, Y. Kamagala, K. Asano, F. Tomita, and A. Yokota. 2006. Modulation of rat cecal microbiota by administration of raffinose and encapsulated Bifidobacterium breve. Appl. Environ. Microbiol. 72:784-792.
9. Franks, A. H., H. J. M. Harmsen, G. C. Raangs, G. J. Jansen, F. Schut, and G. W. Welling. 1998. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 64:3336-3345.
10. Hayashi, H., M. Sakamoto, and Y. Benno. 2002. Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods. Microbiol. Immunol. 46:535-548.
11. Langendijk, P. S., F. Schut, G. J. Jansen, G. C. Raangs, G. R. Kamphuis, M. H. F. Wilkinson, and G. W. Welling. 1995. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl. Environ. Microbiol. 61:3069-3075.
12. Lay, C., L. Rigottier-Gois, K. Holmstrøm, M. Rajilic, E. E. Vaughan, W. M. de Vos, M. D. Collins, R. Thiel, P. Namsolleck, M. Blaut, and J. Doré. 2005. Colonic microbiota signatures across five Northern European countries. Appl. Environ. Microbiol. 71:4153-4155.
13. Lay, C., M. Sutren, V. Rochet, K. Saunier, J. Doré, and L. Rigottier-Gois. 2005. Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota. Environ. Microbiol. 7:933-946.
14. Manz, W., R. Amann, W. Ludwig, M. Vancanneyt, and K. H. Schleifer. 1996. Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology 142:1097-1106.
15. Matsuki, T., K. Watanabe, J. Fujimoto, Y. Kado, T. Takada, K. Matsumoto, and R. Tanaka. 2004. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria. Appl. Environ. Microbiol. 70:167-173.
16. Matsuki, T., K. Watanabe, R. Tanaka, M. Fukuda, and H. Oyaizu. 1999. Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Appl. Environ. Microbiol. 65:4506-4512.
17. Mueller, S., K. Saunier, C. Hanisch, E. Norin, L. Aim, T. Midtvedt, A. Cresci, S. Silvi, C. Orpianesi, M. C. Verdenelli, T. Clavel, C. Koebnick, H. J. F. Zunft, J. Doré, and M. Blaut. 2006. Differences in fecal microbiota in different European study populations in relation to age, gender, and country: a cross-sectional study. Appl. Environ. Microbiol. 72:1027-1033.
18. Nagura, T., H. Muraguchi, K. Uchino, T. Aritsuka, and Y. Benno. 1999. Effects of ingestion of raffinose-rich soup on the fecal flora and daily defecation in humans. J. Intest. Microbiol. 13:1-7. (In Japanese.)
19. Nagura, T., S. Hachimura, M. Hashiguchi, Y. Ueda, T. Kanno, H. Kikuchi, K. Sayama, and S. Kaminogawa. 2002. Suppressive effect of dietary raffinose on T-helper 2 cell-mediated immunity. Br. J. Nutr. 88:421-426.
20. Rathbone, E. B. 1980. Raffinose and melezitose, p. 145-185. In C. K. Lee (ed.), Developments in food carbohydrate, vol. 2. Applied Science Publishers, Ltd., London, United Kingdom.
21. Reddy, N. R., S. K. Sathe, and D. K. Salunkhe. 1989. Carbohydrates, p. 51-74. In D. K. Salunkhe and S. S. Kadam (ed.), CRC handbook of world food legumes: nutritional chemistry, processing technology, and utilization, vol. 1. CRC Press, Inc., Boca Raton, Fla.
22. Rigottier-Gois, L., A. G. Le Bourhis, G. Gramet, V. Rochet, and J. Doré. 2003. Fluorescent hybridisation combined with flow cytometry and hybridisation of total RNA to analyse the composition of microbial communities in human faeces using 16S rRNA probes. FEMS Microbiol. Ecol. 43:237-245.
23. Rigottier-Gois, L., V. Rochet, N. Garrec, A. Suau, and J. Doré. 2003. Enumeration of Bacteroides species in human faeces by fluorescent in situ hybridisation combined with flow cytometry using 16S rRNA probes. Syst. Appl. Microbiol. 26:110-118.
24. Rochet, V., L. Rigottier-Gois, S. Rabot, and J. Doré. 2004. Validation of fluorescent in situ hybridization combined with flow cytometry for assessing interindividual variation in the composition of human fecal microflora during long-term storage of samples. J. Microbiol. Methods 59:263-270.
25. Scardovi, V., and F. Crociani. 1974. Bifidobacterium catenulatum, Bifidobacterium dentium, and Bifidobacterium angulatum: three new species and their deoxyribonucleic acid homology relationships. Int. J. Syst. Bacteriol. 24:6-20.
26. Suau, A., R. Bonnet, M. Sutren, J. J. Godon, G. R. Gibson, M. D. Collins, and J. Doré. 1999. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 65:4799-4807.
27. Suksomcheep, A., I. N. Sujaya, K. Saito, A. Yokota, K. Asano, and F. Tomita. 2001. Development of 16S rDNA targeted probes for detection of some human health beneficial strains of intestinal microorganisms. Biotechnol. Sust. Util. Biol. Resour. Trop. 15:111-125.
28. Takada, T., K. Matsumoto, and K. Nomoto. 2004. Development of multicolor FISH method for analysis of seven Bifidobacterium species in human feces. J. Microbiol. Methods 58:413-421.
29. Wallner, G., R. Amann, and W. Beisker. 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136-143.
30. Zoetendal, E. G., K. Ben-Amor, H. J. M. Harmsen, F. Schut, A. D. L. Akkermans, and W. M. de Vos. 2002. Quantification of uncultured Ruminococcus obeum-like bacteria in human fecal samples by fluorescent in situ hybridization and flow cytometry using 16S rRNA-targeted probes. Appl. Environ. Microbiol. 68:4225-4232.