Functional characterization of ecdysone receptor gene switches in mammalian cells

FEBS Journal

Volumn 273, Issue 24, 2006, Pages 5550-5563

  Panguluri, Siva K ^a   Kumar, Prasanna ^b   Palli, Subba R ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

^b RheoGene Inc   (United States)

Author keywords

ChIP assay; EcR; Gene switch; RNAi; RXR

Indexed keywords

DNA BINDING PROTEIN; ECDYSONE RECEPTOR; HETERODIMER; HYBRID PROTEIN; INSECT PROTEIN; LIGAND; MUTANT PROTEIN; RETINOID X RECEPTOR; RH 2485; TRANSCRIPTION FACTOR GAL4; ULTRASPIRACLE;

ANIMAL CELL; ARTICLE; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; DNA RESPONSIVE ELEMENT; GENE EXPRESSION REGULATION; GENE FUNCTION; GENE SWITCHING; HUMAN; HUMAN CELL; LIGAND BINDING; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; RNA INTERFERENCE;

ANIMALS; GENE EXPRESSION REGULATION; GENES, SWITCH; HUMANS; LIGANDS; MICE; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; NIH 3T3 CELLS; PROTEIN BINDING; RECEPTORS, STEROID; RECOMBINANT FUSION PROTEINS; RETINOID X RECEPTORS; RNA, SMALL INTERFERING; TRANSGENES;

HOMO SAPIENS; INSECTA; MAMMALIA;

EID: 33845474756     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2006.05545.x     Document Type: Article

References (19)

1. Palli SR, Hormann RE, Schlattner U & Lezzi M (2005) Ecdysteroid receptors and their applications in agriculture and medicine. Vitam Horm 73, 59-100.
2. Mangelsdorf DJ, Thummel C, Beato M, Herrlich P, Schutz G, Umesono K, Blumberg B, Kastner P, Mark M & Chambon P (1995) The nuclear receptor superfamily: the second decade. Cell 83, 835-839.
3. Lafont R & Dinan L (2003) Practical uses for ecdysteroids in mammals including humans: an update. J Insect Sci 3, 7.
4. Christopherson KS, Mark MR, Bajaj V & Godowski PJ (1992) Ecdysteroid-dependent regulation of genes in mammalian cells by a Drosophila ecdysone receptor and chimeric transactivators. Proc Natl Acad Sci USA 89, 6314-6318.
5. No D, Yao TP & Evans RM (1996) Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci USA 93, 3346-3351.
6. Suhr ST, Gil EB, Senut MC & Gage FH (1998) High level transactivation by a modified Bombyx ecdysone receptor in mammalian cells without exogenous retinoid X receptor. Proc Natl Acad Sci USA 95, 7999-8004.
7. Hoppe UC, Marban E & Johns DC (2000) Adenovirus-mediated inducible gene expression in vivo by a hybrid ecdysone receptor. Mol Ther 1, 159-164.
8. Wyborski DL, Bauer JC & Vaillancourt P (2001) Bicistronic expression of ecdysone-inducible receptors in mammalian cells. Biotechniques 31, 618-620, 622, 624.
9. Palli SR, Kapitskaya MZ, Kumar MB & Cress DE (2003) Improved ecdysone receptor-based inducible gene regulation system. Eur J Biochem 270, 1308-1315.
10. Karns LR, Kisielewski A, Gulding KM, Seraj JM & Theodorescu D (2001) Manipulation of gene expression by an ecdysone-inducible gene switch in tumor xenografts. BMC Biotechnol 1, 11.
11. Palli SR, Kapitskaya MZ & Potter DW (2005) The influence of heterodimer partner ultraspiracle/retinoid X receptor on the function of ecdysone receptor. FEBS J 272, 5979-90.
12. Karzenowski D, Potter DW & Padidam M (2005) Inducible control of transgene expression with ecdysone receptor: gene switches with high sensitivity, robust expression, and reduced size. Biotechniques 39, 191-192, 194, 196 passim.
13. Toniatti C, Bujard H, Cortese R & Ciliberto G (2004) Gene therapy progress and prospects: transcription regulatory systems. Gene Ther 11, 649-657.
14. Winkler GS, Mulder KW, Bardwell VJ, Kalkhoven E & Timmers HT (2006) Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription. Embo J 25, 3089-3099.
15. Kumar MB, Fujimoto T, Potter DW, Deng Q & Palli SR (2002) A Single Point Mutation in Ecdysone Receptor Leads to Increased Ligand Specificity: Implications for Gene Switch Applications. Proc Natl Acad Sci USA 99, 14710-14715.
16. Lezzi M, Bergman T, Henrich VC, Vogtli M, Fromel C, Grebe M, Przibilla S & Spindler-Barth M (2002) Ligand-induced heterodimerization between the ligand binding domains of the Drosophila ecdysteroid receptor and ultraspiracle. Eur J Biochem 269, 3237-3245.
17. Minakuchi C, Nakagawa Y, Kiuchi M, Tomita S & Kamimura M (2002) Molecular cloning, expression analysis and functional confirmation of two ecdysone receptor isoforms from the rice stem borer Chilo suppressalis. Insect Biochem Mol Biol 32, 999-1008.
18. Perera SC, Ladd TR, Dhadialla TS, Krell PJ, Sohi SS, Retnakaran A & Palli SR (1999) Studies on two ecdysone receptor isoforms of the spruce budworm, Choristoneura fumiferana. Mol Cell Endocrinol 152, 73-84.
19. Kothapalli R, Palli SR, Ladd TR, Sohi SS, Cress D, Dhadialla TS, Tzertzinis G & Retnakaran A (1995) Cloning and developmental expression of the ecdysone receptor gene from the spruce budworm, Choristoneura fumiferana. Dev Genet 17, 319-330.