A phosphorylation site in the Toll-like receptor 5 TIR domain is required for inflammatory signalling in response to flagellin

Biochemical and Biophysical Research Communications

Volumn 352, Issue 4, 2007, Pages 936-941

  Ivison, Sabine M ^a   Khan, Mohammed A S ^a,b   Graham, Nicholas R ^a   Bernales, Cecily Q ^a   Kaleem, Arnawaz ^a   Tirling, Chelsea O ^a   Cherkasov, Artem ^a   Steiner, Theodore S ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b BC CHILDREN S HOSPITAL   (Canada)

Author keywords

Chemokines; Epithelial cells; Inflammation; Innate immunity; Kinases; Mass spectrometry; Signal transduction

Indexed keywords

FLAGELLIN; INTERLEUKIN 1 RECEPTOR; PHOSPHATIDYLINOSITOL 3 KINASE; RECEPTOR SUBTYPE; TOLL LIKE RECEPTOR 5; TYROSINE;

ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; DIMERIZATION; HOMODIMERIZATION; HUMAN; HUMAN CELL; INFLAMMATION; INTRACELLULAR TRANSPORT; MAMMAL CELL; MASS SPECTROMETER; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; RECEPTOR BINDING; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CELL LINE; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; FLAGELLIN; HUMANS; INFLAMMATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SIGNAL TRANSDUCTION; TOLL-LIKE RECEPTOR 5; TYROSINE;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 33845450196     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.11.132     Document Type: Article

References (16)

1. Berin M.C., Darfeuille-Michaud A., Egan L.J., Miyamoto Y., and Kagnoff M.F. Role of EHEC O157:H7 virulence factors in the activation of intestinal epithelial cell NF-kappaB and MAP kinase pathways and the upregulated expression of interleukin 8. Cell. Microbiol. 4 (2002) 635-648
2. Yu Y., Zeng H., Lyons S., Carlson A., Merlin D., Neish A.S., and Gewirtz A.T. TLR5-mediated activation of p38 MAPK regulates epithelial IL-8 expression via a post-transcriptional mechanism. Am. J. Physiol. Gastrointest. Liver Physiol. 17 (2003) 17
3. Khan M.A., Kang J., and Steiner T.S. Enteroaggregative Escherichia coli flagellin-induced interleukin-8 secretion requires Toll-like receptor 5-dependent p38 MAP kinase activation. Immunology 112 (2004) 651-660
4. Hayashi F., Smith K.D., Ozinsky A., Hawn T.R., Yi E.C., Goodlett D.R., Eng J.K., Akira S., Underhill D.M., and Aderem A. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 410 (2001) 1099-1103
5. Mizel S.B., Honko A.N., Moors M.A., Smith P.S., and West A.P. Induction of macrophage nitric oxide production by gram-negative flagellin involves signaling via heteromeric toll-like receptor 5/toll-like receptor 4 complexes. J. Immunol. 170 (2003) 6217-6223
6. Rhee S.H., Kim H., Moyer M.P., and Pothoulakis C. Role of MyD88 in phosphatidylinositol 3-kinase activation by flagellin/toll-like receptor 5 engagement in colonic epithelial cells. J. Biol. Chem. 281 (2006) 18560-18568 Epub 12006 Apr 18527
7. Yu Y., Nagai S., Wu H., Neish A.S., Koyasu S., and Gewirtz A.T. TLR5-mediated phosphoinositide 3-kinase activation negatively regulates flagellin-induced proinflammatory gene expression. J. Immunol. 176 (2006) 6194-6201
8. Sarkar S.N., Peters K.L., Elco C.P., Sakamoto S., Pal S., and Sen G.C. Novel roles of TLR3 tyrosine phosphorylation and PI3 kinase in double-stranded RNA signaling. Nat. Struct. Mol. Biol. 11 (2004) 1060-1067 Epub 2004 Oct 1024
9. Marmiroli S., Bavelloni A., Faenza I., Sirri A., Ognibene A., Cenni V., Tsukada J., Koyama Y., Ruzzene M., Ferri A., Auron P.E., Toker A., and Maraldi N.M. Phosphatidylinositol 3-kinase is recruited to a specific site in the activated IL-1 receptor I. FEBS Lett. 438 (1998) 49-54
10. Donnelly M.A., and Steiner T.S. Two nonadjacent regions in enteroaggregative Escherichia coli flagellin are required for activation of toll-like receptor 5. J. Biol. Chem. 277 (2002) 40456-40461
11. Xu Y., Tao X., Shen B., Horng T., Medzhitov R., Manley J.L., and Tong L. Structural basis for signal transduction by the Toll/interleukin-1 receptor domains. Nature 408 (2000) 111-115
12. Cherkasov A. Inductive electronegativity scale. Iterative calculation of inductive partial charges. J. Chem. Inf. Comput. Sci. 43 (2003) 2039-2047
13. Cherkasov A. 'Inductive' descriptors. 10 successful years in QSAR. Curr. Comput.-Aided Drug Design 1 (2005) 21-42
14. Okugawa S., Yanagimoto S., Tsukada K., Kitazawa T., Koike K., Kimura S., Nagase H., Hirai K., and Ota Y. Bacterial flagellin inhibits T cell receptor-mediated activation of T cells by inducing suppressor of cytokine signalling-1 (SOCS-1). Cell Microbiol. 8 (2006) 1571-1580
15. Arbibe L., Mira J.P., Teusch N., Kline L., Guha M., Mackman N., Godowski P.J., Ulevitch R.J., and Knaus U.G. Toll-like receptor 2-mediated NF-kappa B activation requires a Rac1-dependent pathway. Nat. Immunol. 1 (2000) 533-540
16. Sarkar S.N., Smith H.L., Rowe T.M., and Sen G.C. Double-stranded RNA signaling by Toll-like receptor 3 requires specific tyrosine residues in its cytoplasmic domain. J. Biol. Chem. 278 (2003) 4393-4396 Epub 2002 Dec 4330