Mutations in the inter-SH2 domain of the regulatory subunit of phosphoinositide 3-kinase: Effects on catalytic subunit binding and holoenzyme function

Biological Chemistry

Volumn 387, Issue 12, 2006, Pages 1567-1573

  Elis, Winfried ^a,c   Lessmann, Eva ^a   Oelgeschlager, Michael ^b   Huber, Michael ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b MAX PLANCK INSTITUTE OF IMMUNOBIOLOGY AND EPIGENETICS   (Germany)

^c NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Dominant negative; Tandem affinity purification; Xbra; Xenopus development

Indexed keywords

PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN P110; PROTEIN P85; PROTEIN SH2;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL FUNCTION; CONTROLLED STUDY; ENZYME BINDING; ENZYME REGULATION; ENZYME SUBUNIT; GENE MUTATION; HYDROPHOBICITY; IN VITRO STUDY; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; XENOPUS;

1-PHOSPHATIDYLINOSITOL 3-KINASE; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATALYSIS; CERCOPITHECUS AETHIOPS; COS CELLS; DIMERIZATION; DNA PRIMERS; MOLECULAR SEQUENCE DATA; MUTATION; SIGNAL TRANSDUCTION; SRC HOMOLOGY DOMAINS; SUBSTRATE SPECIFICITY;

EID: 33751532426     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2006.195     Document Type: Article

References (26)

1. Agius, E., Oelgeschlager, M., Wessely, O., Kemp, C., and De Robertis, E.M. (2000). Endodermal nodal-related signals and mesoderm induction in Xenopus. Development 127, 1173-1183.
2. Cantley, L.C. (2002). The phosphoinositide 3-kinase pathway. Science 296, 1655-1657.
3. Carballada, R., Yasuo, H., and Lemaire, P. (2001). Phosphatidylinositol-3 kinase acts in parallel to the ERK MAP kinase in the FGF pathway during Xenopus mesoderm induction. Development 128, 35-44.
4. Chan, T.O., Rodeck, U., Chan, A.M., Kimmelman, A.C., Rittenhouse, S.E., Panayotou, G., and Tsichlis, P.N. (2002). Small GTPases and tyrosine kinases coregulate a molecular switch in the phosphoinositide 3-kinase regulatory subunit. Cancer Cell 1, 181-191.
5. Dhand, R., Hara, K., Hiles, I., Bax, B., Gout, I., Panayotou, G., Fry, M.J., Yonezawa, K., Kasuga, M., and Waterfield, M.D. (1994). PI 3-kinase: structural and functional analysis of intersubunit interactions. EMBO J. 13, 511-521.
6. Elis, W., Reth, M., and Huber, M. (2004). Functional folding of a cytoplasmic single-chain variable fragment and its use as elutable protein purification tag. Immunol. Lett. 92, 185-192.
7. Fu, Z., Aronoff-Spencer, E., Wu, H., Gerfen, G.J., and Backer, J.M. (2004). The iSH2 domain of PI 3-kinase is a rigid tether for p110 and not a conformational switch. Arch. Biochem. Biophys. 432, 244-251.
8. Fukazawa, T., Miyake, S., Band, V., and Band, H. (1996). Tyrosine phosphorylation of Cbl upon epidermal growth factor (EGF) stimulation and its association with EGF receptor and downstream signaling proteins. J. Biol. Chem. 271, 14554-14559.
9. Hill, K.M., Huang, Y., Yip, S.C., Yu, J., Segall, J.E., and Backer, J.M. (2001). N-terminal domains of the class Ia phosphoinositide 3-kinase regulatory subunit play a role in cytoskeletal but not mitogenic signaling. J. Biol. Chem. 276, 16374-16378.
10. Holt, K.H., Olson, L., Moye-Rowley, W.S., and Pessin, J.E. (1994). Phosphatidylinositol 3-kinase activation is mediated by high-affinity interactions between distinct domains within the p110 and p85 subunits. Mol. Cell. Biol. 14, 42-49.
11. Hu, P., Mondino, A., Skolnik, E.Y., and Schlessinger, J. (1993). Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. Mol. Cell. Biol. 13, 7677-7688.
12. Jimenez, C., Portela, R.A., Mellado, M., Rodriguez-Frade, J.M., Collard, J., Serrano, A., Martinez, A.C., Avila, J., and Carrera, A.C. (2000). Role of the PI3K regulatory subunit in the control of actin organization and cell migration. J. Cell. Biol. 151, 249-262.
13. Kang, H., Schneider, H., and Rudd, C.E. (2002). Phosphatidylinositol 3-kinase p85 adaptor function in T-cells. Co-stimulation and regulation of cytokine transcription independent of associated p110. J. Biol. Chem. 277, 912-921.
14. Klippel, A., Escobedo, J.A., Hirano, M., and Williams, L.T. (1994). The interaction of small domains between the subunits of phosphatidylinositol 3-kinase determines enzyme activity. Mol. Cell. Biol. 14, 2675-2685.
15. Luo, J., Field, S.J., Lee, J.Y., Engelman, J.A., and Cantley, L.C. (2005). The p85 regulatory subunit of phosphoinositide 3-kinase down-regulates IRS-1 signaling via the formation of a sequestration complex. J. Cell Biol. 170, 455-464.
16. Nieuwkoop, P. and Faber, J. (1994). Normal Table of Xenopus laevis (New York, USA: Garland Publishing Inc.).
17. Okkenhaug, K. and Vanhaesebroeck, B. (2003). PI3K in lymphocyte development, differentiation and activation. Nat. Rev. Immunol. 3, 317-330.
18. Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. (1999). A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17, 1030-1032.
19. Rordorf-Nikolic, T., Van Horn, D.J., Chen, D., White, M.F., and Backer, J.M. (1995). Regulation of phosphatidylinositol 3′-kinase by tyrosyl phosphoproteins. Full activation requires occupancy of both SH2 domains in the 85-kDa regulatory subunit. J. Biol. Chem. 270, 3662-3666.
20. Sive, H.L., Grainger, R.M., and Harland, R.M. (2000). Early Development of Xenopus laevis: A Laboratory Manual (New York, USA: Cold Spring Harbor Laboratory Press).
21. 2+ influx by enhancing inositol 1,4,5-trisphosphate production. J. Biol. Chem. 276, 17213-17220.
22. Smith, J.C., Price, B.M., Green, J.B., Weigel, D., and Herrmann, B.G. (1991). Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. Cell 67, 79-87.
23. Vanhaesebroeck, B., Leevers, S.J., Ahmadi, K., Timms, J., Katso, R., Driscoll, P.C., Woscholski, R., Parker, P.J., and Waterfield, M.D. (2001). Synthesis and function of 3-phosphorylated inositol lipids. Annu. Rev. Biochem. 70, 535-602.
24. Yu, J., Wjasow, C., and Backer, J.M. (1998a). Regulation of the p85/p110α phosphatidylinositol 3′-kinase. Distinct roles for the N-terminal and C-terminal SH2 domains. J. Biol. Chem. 273, 30199-30203.
25. Yu, J., Zhang, Y., McIlroy, J., Rordorf-Nikolic, T., Orr, G.A., and Backer, J.M. (1998b). Regulation of the p85/p110 phosphatidylinositol 3′-kinase: stabilization and inhibition of the p110α catalytic subunit by the p85 regulatory subunit. Mol. Cell. Biol. 18, 1379-1387.
26. Zieger, M., Oehrl, W., Wetzker, R., Henklein, P., Nowak, G., and Kaufmann, R. (2000). Different signaling pathways are involved in CCK(B) receptor-mediated MAP kinase activation in COS-7 cells. Biol. Chem. 381, 763-768.