Fluorescent protein pair emit intracellular FRET signal suitable for FACS screening

Biochemical and Biophysical Research Communications

Volumn 352, Issue 2, 2007, Pages 449-455

  Johansson, Daniel X ^a   Brismar, Hjalmar ^a,b   Persson, Mats A A ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Antibodies; FACS; FRET; HIV 1; Membrane proteins; Protein protein interactions

Indexed keywords

MEMBRANE PROTEIN;

ARTICLE; BLEACHING; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; MAMMAL CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION;

CELL SEPARATION; ENDOPLASMIC RETICULUM; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMALIA;

EID: 33751529745     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.11.042     Document Type: Article

References (17)

1. Anderson D.J., and Blobel G. Immunoprecipitation of proteins from cell-free translations. Methods Enzymol. 96 (1983) 111-120
2. Fields S., and Song O. A novel genetic system to detect protein-protein interactions. Nature 340 (1989) 245-246
3. Vukojevic V., Pramanik A., Yakovleva T., Rigler R., Terenius L., and Bakalkin G. Study of molecular events in cells by fluorescence correlation spectroscopy. Cell. Mol. Life Sci. 62 5 (2005) 535-550
4. Remy I., and Michnick S.W. Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays. Proc. Natl. Acad. Sci. USA 96 (1999) 5394-5399 Erratum in: Proc. Natl. Acad. Sci. USA 1999 96(13) 7610
5. Mahajan N.P., Linder K., Berry G., Gordon G.W., Heim R., and Herman B. Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. Nat. Biotechnol. 16 (1998) 547-552
6. Clegg R.M. Fluorescence resonance energy transfer. Curr. Opin. Biotechnol. 6 (1995) 103-110
7. Liao K.W., Chou W.C., Lo Y.C., and Roffler S.R. Design of transgenes for efficient expression of active chimeric proteins on mammalian cells. Biotechnol. Bioeng. 73 (2001) 313-323
8. Samuelsson A., Yari F., Hinkula J., Ersoy O., Norrby E., and Persson M.A.A. Human antibodies from phage libraries: neutralizing activity against human immunodeficiency virus type 1 equally improved after expression as Fab and IgG in mammalian cells. Eur. J. Immunol. 26 (1996) 3029-3034
9. Adachi A., Gendelman H.E., Koenig S., Folks T., Willey R., Rabson A., and Martin M.A. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J. Virol. 59 (1986) 284-291
10. Felber B.K., Drysdale C.M., and Pavlakis G.N. Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein. J. Virol. 64 (1990) 3734-3741
11. Åkerblom L., Hinkula J., Broliden P.A., Makitalo B., Fridberger T., Rosen J., Villacres-Eriksson M., Morein B., and Wahren B. Neutralizing cross-reactive and non-neutralizing monoclonal antibodies to HIV-1 gp120. AIDS 4 (1990) 953-960
12. Bordier C. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem. 256 (1981) 1604-1607
13. Ng T., Squire A., Hansra G., Bornancin F., Prevostel C., Hanby A., Harris W., Barnes D., Schmidt S., Mellor H., et al. Imaging protein kinase Calpha activation in cells. Science 283 (1999) 2085-2089
14. Jovin T.M., and Arndt-Jovin D.J. Luminescence digital imaging microscopy. Annu. Rev. Biophys. Biophys. Chem. 18 (1989) 271-308
15. Rasband, W.S., ImageJ, US National Institutes of Health, Bethesda, Maryland, USA, , 1997-2006.
16. Persson M.A.A., Caothien R.H., and Burton D.R. Generation of diverse high affinity human monoclonal antibodies by repertoire cloning. Proc. Natl. Acad. Sci. USA 88 (1991) 2432-2436
17. Nord K., Gunneriusson E., Ringdahl J., Stahl S., Uhlen M., and Nygren P.A. Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat. Biotechnol. 15 (1997) 772-777