Validation of the PCR-based universal heteroduplex generator assay for identification of rifampicin-resistant and multiresistant Mycobacterium tuberculosis in Lima, Peru;Validación de la reacción en cadena de la polimerasa basada en generador universal de heterodúplex para la identificación de Mycobacterium tuberculosis resistente a rifampicina y multirresistente en Lima, Perú

Enfermedades Infecciosas y Microbiologia Clinica

Volumn 24, Issue 8, 2006, Pages 495-499

  Calderón Espinoza, Róger I ^a,b   Asencios Solís, Luis ^a   Quispe Torres, Neyda ^a   Yale Cajahuanca, Gloria ^a   Suárez Nole, Carmen ^a   Lecca García, Leonid ^a   Llanos Zavalaga, Luis F ^a  

^a UNIVERSIDAD PERUANA CAYETANO HEREDIA   (Peru)

^b INSTITUTO NACIONAL DE SALUD   (Peru)

Author keywords

Multidrug resistance; PCR UHG Rif; Rifampin resistance; Tuberculosis

Indexed keywords

ISONIAZID; RIFAMPICIN; STREPTOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; DRUG SENSITIVITY; HETERODUPLEX ANALYSIS; HUMAN; INCIDENCE; LUNG TUBERCULOSIS; MISSENSE MUTATION; MYCOBACTERIUM TUBERCULOSIS; PERU; POINT MUTATION; POLYMERASE CHAIN REACTION; VALIDATION STUDY;

EID: 33750986229     PISSN: 0213005X     EISSN: None     Source Type: Journal    
DOI: 10.1157/13092465     Document Type: Article

References (16)

1. Rattan A, Kalia A, Ahmad N. Multidrug resistant Mycobacterium tuberculosis: Molecular perspectives. Emerg Infect Dis. 1998;4(2):195-209.
2. Vásquez-Campos L, Asencios-Solis L, Leo-Hurtado E, Quispe-Torres N, Salazar-Lindo E, Bayona J, et al. Drug resistance trends among previously treated tuberculosis patients in a national registry in Peru, 1994-2001. Int J Tuberc Lung Dis. 2004;8(4):465-72.
3. Farmer P, Shin S, Bayona J, Kim J, Furin J, Brenner J. Making DOTS Plus work. En: Multidrug-resistant tuberculosis. Bastian I, Portaels F, editors. Dordrech: Kluewer Acad. Publishers; 2000.
4. Ramaswamy S, Musser J. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuberc and Lung Dis. 1998;79:3-29.
5. Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicrobial resistance. Clin Microbiol Rev. 2001;14:836-71.
6. Cole S, Telenti A. Drug resistance in Mycobacterium tuberculosis. Eur Res J. 1995;20:701-13.
7. Williams DL, Limbers C, Spring L, Jayachandra S, Gillis T. PCR/Heteroduplex detection of rifampin-resistant Mycobacterium tuberculosis. En: PCR protocol for emerging infectious diseases. Persing D, editor. ASM; 1994. p. 122-9.
8. Strong B, Kubica G. Isolation and identification of Mycobacterium tuberculosis: A guide for the level II laboratory. Atlanta, GA: DHHS. Centers for Disease Control; 1981.
9. Williams DL, Gillis TP, Dupree WG. Ethanol fixation of sputum sediments for DNA-based detection of Mycobacterium tuberculosis. J Clin Microbiol. 1995;33:1558-61.
10. Whelen AC, Felmlee TA, Hunt JM, Williams DL, Roberts GD, Stockman L, et al. Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single-tube heminested PCR. J Clin Microbiol. 1995;33:556-61.
11. Calderon R, Asencios L, Quispe N, Custodio W, Montoya Y. Detección rapida de resistencia a drogas en Mycobacterium tuberculosis mediante PCR-SSCP y PCR-heterodúplex. Rev Peruana Med Exp Salud Pública. 2003;20:65-71 (Disponible en http://wvw.scielo.org.pe/pdf/rins/v20n2/a02v20n2. pdf).
12. Mayta H, Gilman R, Arenas F, Valencia T, Caviedes L, Montenegro S, et al. Evaluation of a PCR-Based Universal Heteroduplex Generator Assay as a Tool for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis in Peru. J Clin Microbiol. 2003;41:5774-7.
13. Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicrobial resistance. Clin Microbiol Rev. 2001;14:836-71.
14. Heep M, Brandstatter B, Rieger U, Lehn N, Richter E, Rusch-Gerdes S, et al. Frequency of rpoB mutations inside and outside the cluster I region in rifampin-resistant clinical Mycobacterium tuberculosis isolates. J Clin Microbiol. 2001;39:107-10.
15. Kiepiela P, Bishop K, Kormuth E, Roux L, York DF. Comparison of PCR-heteroduplex characterization by automated DNA sequencing and line probe assay for the detection of rifampicin resistance in Mycobacterium tuberculosis isolates from KwaZulu-Natal, South Africa. Microb Drug Resist. 1998;4:263-9.
16. Campos P, Suárez P, Sánchez J, Zavala D, Arévalo J, Ticona E, et al. Multidrug-resistant Mycobacterium tuberculosis in HIV-Infected Persons, Peru. Emerg Infect Dis. 2003;9:1571-8.