Flow cytometric and morphological characterization of platelet-rich plasma gel

Clinical Oral Implants Research

Volumn 17, Issue 6, 2006, Pages 687-693

  Fernández Barbero, Juan Emilio ^a   Galindo Moreno, Pablo ^a,c   Ávila Ortiz, Gustavo ^a   Caba, Octavio ^a   Sanchez Fernandez E ^a   Wang, Hom Lay ^b  

^a UNIVERSITY OF GRANADA   (Spain)

^b UNIVERSITY OF MICHIGAN   (United States)

^c NONE   (Spain)

Author keywords

Growth factors; Platelet rich plasma (PRP); Tissue engineering; Wound healing

Indexed keywords

DIAGNOSTIC AGENT; GLYCOPROTEIN IIB; MONOCLONAL ANTIBODY;

ANIMAL; ARTICLE; CHEMISTRY; FLOW CYTOMETRY; GEL; HUMAN; MOUSE; SCANNING ELECTRON MICROSCOPY; THROMBOCYTE RICH PLASMA; TRANSMISSION ELECTRON MICROSCOPY;

ANIMALS; ANTIBODIES, MONOCLONAL; FLOW CYTOMETRY; GELS; HUMANS; MICE; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; PLATELET MEMBRANE GLYCOPROTEIN IIB; PLATELET-RICH PLASMA;

EID: 33750703613     PISSN: 09057161     EISSN: 16000501     Source Type: Journal    
DOI: 10.1111/j.1600-0501.2006.01179.x     Document Type: Article

References (61)

1. Aghaloo, T.L., Moy, P.K. & Freymiller, E.G. (2002) Investigation of platelet-rich plasma in rabbit cranial defects: a pilot study. Journal of Oral and Maxillofacial Surgery 60: 1176-1181.
2. Anitua, E. (1999) Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. International Journal of Oral & Maxillofacial Implants 14: 529-535.
3. Anitua, E. (2001) The use of plasma-rich growth factors (PRGF) in oral surgery. Practical Procedures in Aesthetic Dentistry 13: 487-493.
4. Anitua, E., Andia, I., Ardanza, B., Nurden, P. & Nurden, A.T. (2004) Autologous platelets as a source of proteins for healing and tissue regeneration. Thrombosis and Haemostasis 91: 4-15.
5. Appel, T.R., Pötzsch, B., Müller, J., von Lindern, J-J., Bergé, S.J. & Reich, R.H. (2002) Comparison of three different preparations of platelet concentrates for growth factor enrichment. Clinical Oral Implants Research 13: 522-528.
6. Arpornmaeklong, R., Kochel, M., Depprich, R., Kübler, N.R. & Würzler, K.K. (2004) Influence of platelet-rich plasma (PRP) on osteogenic differentiation of rat bone marrow stromal cells. An in vitro study. International Journal of Oral and Maxillofacial Surgery 33: 60-70.
7. Bennett, N.T. & Schultz, G.S. (1993a) Growth factors and wound healing: part I. Biochemical properties of growth factors and their receptors. American Journal of Surgery 165: 728-37.
8. Bennett, N.T. & Schultz, G.S. (1993b) Growth factors and wound healing: part II. Role in normal and chronic wound healing. American Journal of Surgery 166: 74-81.
9. Bloom, S., Holmstrup, P. & Dabelsteen, E. (1994) A comparison of the effect of epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor on rat periodontal ligament fibroblast-like cells DNA synthesis and morphology. Journal of Periodontology 65: 373-378.
10. Broberg, M., Eriksson, C. & Nygren, H. (2002) GpIIb/IIIa is the main receptor for initial platelet adhesion to glass and titanium surfaces in contact with whole blood. Journal of Laboratory and Clinical Medicine 139: 163-172.
11. Carlson, N.E. & Roach, R.B. Jr (2002) Platelet-rich plasma: clinical applications in dentistry. Journal of the American Dental Association 133: 1383-1386.
12. Chapman, E.S., Sorette, M., Hetherington, E., Zelmanovic, D., Kling, G., Dugailliez, J., Pujol-Moix, N. & Okrongly, D. (2003) A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination. Thrombosis and Haemostasis 89: 1004-1015.
13. Choi, B.H., Im, C.J., Huh, J.Y., Suh, J.J. & Lee, S.H. (2004) Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. International Journal of Oral and Maxillofacial Surgery 33: 56-59.
14. Coller, B.S. (1990) Platelets and thrombolytic therapy. New England Journal of Medicine 322: 33-42.
15. Della Valle, A., Sammartino, G., Marenzi, G., Tia, M., Espedito di Lauro, A., Ferrari, F. & LoMuzio, L. (2003) Prevention of postoperative bleeding in anticoagulated patients undergoing oral surgery: use of platelet-rich plasma gel. Journal of Oral and Maxillofacial Surgery 61: 1275-1278.
16. De Obarrio, J.J., Arauz-Dutari, J.I., Chamberlain, T.M. & Croston, A. (2000) The use of autologous growth factors in periodontal surgical therapy: platelet gel biotechnology-case reports. International Journal of Periodontics and Restorative Dentistry 20: 486-497.
17. Diaz-Flores, L., Ortiz-Urdiain, G. & González- Fernández, A. (1974) Ultraestructura del Sinoviosarcoma: estirpes celulares. Revista Ibys 32: 1.
18. Fennis, J.P.M., Stoelinga, P.J.W. & Jansen, J.A. (2004) Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet-rich plasma in goats. International Journal of Oral and Maxillofacial Surgery 33: 48-55.
19. Fernández, J.E., Prados, J., Melguizo, C., Arena, N., Malavasi, F., Álvarez, L. & Aránega, A. (1998) Characterization of a new embryonal rhabdomyosarcoma cell line, RMS-GR. Japan Journal Cancer Research 89: 525-532.
20. Froum, S.J., Wallace, S.S., Tarnow, D.P. & Cho, S. (2002) Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports. International Journal of Periodontics and Restorative Dentistry 22: 45-53.
21. Fürst, G., Gruber, R., Tangl, S., Sanroman, F. & Watzek, G. (2003b) Enhanced bone-to-implant contact bye platelet-released growth factors in mandibular cortical bone: a histomorphometric study in minipigs. International Journal of Oral & Maxillofacial Implants 18: 685-690.
22. Fürst, G., Gruber, R., Tangl, S., Zechner, W., Haas, R., Mailath, G., Sanroman, F. & Watzek, G. (2003a) Sinus grafting with autogenous plateletrich plasma and bovine hidroxyapatite. A histomorphometric study in minipigs. Clinical Oral Implants Research 14: 500-508.
23. Godet, G., Canessa, R., Arock, M., Baron, J.F., Kieffer, E. & Viars, P. (1995) Effects of platele-trich plasma on hemostasis and transfusion requirement in vascular surgery. Annales Françaises D'Anesthesie et de Reanimation 14: 265-270.
24. Gonshor, A. (2002) Technique for producing platelet-rich plasma and platelet concentrate: background and process. International Journal of Periodontics and Restorative Dentistry 22: 547-557.
25. Hawiger, J., Hammond, D.K., Timmons, S. & Budzynski, A.Z. (1978) Interaction of human fibrinogen with staphylococci: presence of a binding region on normal and abnormal fibrinogen variants and fibrinogen derivatives. Blood 51: 799-812.
26. Jakse, N., Tangl, S., Gilli, R., Berghold, A., Lorenzoni, M., Eskici, A., Haas, R. & Pertl, C. (2003) Influence of PRP on autogenous sinus grafts. An experimental study on sheep. Clinical Oral Implants Research 14: 578-583.
27. Kawase, T., Okuda, K., Wolff, L.F. & Yoshie, H. (2003) Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro. Journal of Periodontology 74: 858-864.
28. Kieffer, N. & Phillips, D.R. (1990) Platelet membrane glycoproteins: functions in cellular interactions. Annual Review of Cell Biology 6: 329-357.
29. Landesberg, R., Roy, M. & Glickman, R.S. (2000) Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. Journal of Oral and Maxillofacial Surgery 58: 297-300; discussion 300-301.
30. Lekovic, V., Camargo, P.M., Weinlander, M., Vasilic, N., Aleksic, Z. & Barrie Kenney, E. (2003) Effectiveness of a combination of platelet-rich plasma, bovine porous bone mineral and guided tissue regeneration in the treatment of mandibular grade II molar furcations in humans. Journal of Clinical Periodontology 30: 746-751.
31. Lekovic, V., Camargo, P.M., Weinlaender, M., Vasilic, N. & Barrie Kenney, E. (2002) Comparison of platelet-rich plasma, bovine porous bone mineral and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: a reentry study. Journal of Periodontology 73: 198-205.
32. Leytin, V., Shapiro, H., Novikov, I. & Radnay, J. (1996) Flow cytometric analysis of the platelet surface area and surface density of glycoprotein IIb-IIIa of unactivated human platelets of various sizes. Biochemical and Biophysical Research Community 226: 94-100.
33. Lozada, J.L., Caplanis, N., Proussaefs, P., Willardsen, J. & Kammeyer, G. (2001) Platelet-rich plasma application in sinus graft surgery: part I - Background and processing techniques. Journal of Oral Implantology 27: 38-42.
34. Maiorana, C., Sommariva, L., Brivio, P., Sigurta, D. & Santoro, F. (2003) Maxillary sinus augmentation with anorganic bovine bone (Bio-Oss) and autologous platelet-rich plasma: preliminary clinical and histologic evaluations. International Journal of Periodontics and Restorative Dentistry 23: 227-235.
35. Marx, R.E. (2001) Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dentistry 10: 225-228.
36. Marx, R.E., Carlson, E.R., Eichstaedt, R.M., Schimmele, S.R., Strauss, J.E. & Georgeff, K.R. (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 85: 638-646.
37. Matyus, L., Bene, L., Harsfalvi, J., Alvarez, M.V., Gonzalez-Rodriguez, J., Jenei, A., Muszbek, L. & Damjanovich, S. (2001) Organization of the glycoprotein (GP) IIb/IIIa heterodimer on resting human platelets studied by flow cytometric energy transfer. Journal of Photochemistry and Photobiology: B 65: 47-58.
38. Michelson, A.D. (2000) Flow cytometric analysis of platelets. Vox Sanguinis 78 (Suppl. 2): 137-142.
39. Morita, Y., Yamamura, M., Kawashima, M., Harada, S., Tsuji, K., Shibuya, K., Maruyama, K. & Makino, H. (1998) Flow cytometric single-cell analysis of cytokine production by CD4+ T cells in synovial tissue and peripheral blood from patients with rheumatoid arthritis. Arthritis and Rheumatism 41: 1669-1676.
40. Moritz, A., Walcheck, B.K. & Weiss, D.J. (2003) Flow cytometric detection of activated platelets in the dog. Veterinary Clinical Pathology 32: 6-12.
41. Okuda, K., Kawase, T., Momose, M., Murata, M., Saito, Y., Suzuki, H., Wolff, L.F. & Yoshie, H. (2003) Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. Journal of Periodontology 74: 849-857.
42. Pichler, J., Printz, D., Scharner, D., Trbojevic, D., Siekmann, J. & Fritsch, G. (2002) Improved flow cytometric method to enumerate residual cells: minimal linear detection limits for platelets, erythrocytes, and leukocytes. Cytometry 50: 231-237.
43. Roldán, J.C., Jepsen, S., Miller, J., Freitag, S., Rueger, D.C., Açil, Y. & Terheyden, H. (2004) Bone formation in the presence of platelet-rich plasma vs. morphogenetic protein-7. Bone 34: 80-90.
44. Rosenberg, E.S. & Torosian, J. (2000) Sinus grafting using platelet-rich plasma-initial case presentation. Practical Periodontics in Aesthetic Dentistry 12: 843-850; quiz 852.
45. Rotllan, E., Escolar, G., Ordinas, A. & Bastida, E. (1993) Effect of PAF (platelet-activating factor) on hemostasis. Studies on endothelial cells and platelets. Blood 38: 115-119.
46. Sánchez, A.R., Sheridan, P.J. & Kupp, L.I. (2003) Is platelet-rich plasma the perfect enhancement factor? A current review. International Journal of Oral & Maxillofacial Implants 18: 93-103.
47. Schmitz, J.P. & Hollinger, J.O. (2001) The biology of platelet-rich plasma. Journal of Oral and Maxillofacial Surgery 59: 1119-1121.
48. Shanaman, R., Filstein, M.R. & Danesh-Meyer, M.J. (2001) Localized ridge augmentation using GBR and platelet-rich plasma: case reports. International Journal of Periodontics and Restorative Dentistry 21: 345-355.
49. Soffer, E., Ouhayoun, J.P. & Anagnostou, F. (2003) Fibrin sealants and platelet preparations in bone periodontal healing. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics 95: 521-528.
50. Sonnleitner, D., Huemer, P. & Sullivan, D.Y. (2000) A simplified technique for producing platelet-rich plasma and platelet concentrate for intraoral bone grafting techniques: a technical note. International Journal of Oral & Maxillofacial Implants 15: 879-882.
51. Thomas, R.A., Krishan, A. & Brochu, M. (2002) High resolution flow cytometric analysis of electronic nuclear volume and DNA content in normal and abnormal human tissue. Methods in Cell Science 24: 11-18.
52. Thorn, J.J., Sorensen, U., Weis-Fogh, M. & Andersen, M. (2004) Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery. International Journal of Oral and Maxillofacial Surgery 33: 95-100.
53. Tischler, M. (2002) Platelet rich plasma. The use of autologous growth factors to enhance bone and soft tissue grafts. New York State Dentistry Journal 68: 22-24.
54. Tozum, T.F. & Demiralp, B. (2003) Platelet-rich plasma: a promising innovation in dentistry. Journal of Canadian Dental Association 69: 664.
55. Vanassche, B. & Defrancq, J. (2001) Use of PRP (Platelet Rich Plasma) in bone volume augmentation). Revue Belge de Medecene Dentaire 56: 125-133.
56. Weibrich, G., Kleis, W.K.G., Hafner, G., Hitzler, W.E. & Wagner, W. (2003a) Comparison of platelet, leucocyte and growth factor levels in point-of-care platelet-enriched plasma, prepared using a modified Curasan kit, with preparations received from a local blood bank. Clinical Oral Implants Research 14: 357-362.
57. Weibrich, G., Kleis, W.K.G., Buch, R., Hitzler, W.E. & Hafner, G. (2003b) The Harvest Smart Prept system versus the Friadent-Schütze platelet-rich plasma kit. Comparison of a semiautomatic method with a more complex method for the preparation of platelet concentrates. Clinical Oral Implants Research 14: 233-239.
58. Weibrich, G., Hansen, T., Kleis, W., Buch, R. & Hitzler, W.E. (2004) Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration. Bone 34: 665-671.
59. Wiltfang, J., Kloss, F.R., Kessler, P., Nkenke, E., Schultze-Mosgau, S., Zimmerman, R. & Schlegel, K.A. (2004) Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clinical Oral Implants Research 15: 187-193.
60. Wiltfang, J., Schlegel, K.A., Schultze-Mosgau, S., Nkenke, E., Zimmermann, R. & Kessler, P. (2003) Sinus floor augmentation with beta-tricalciumphosphate (beta-TCP): does platelet-rich plasma promote its osseous integration and degradation? Clinical Oral Implants Research 14: 213-218.
61. Zechner, W., Tangl, S., Tepper, G., Fürst, G., Bernhart, T., Haas, R., Mailath, G. & Watzek, G. (2003) Influence of platelet-rich plasma on osseous healing dental implants: a histologic and histomorphometric study in minipigs. International Journal of Oral & Maxillofacial Implants 18: 15-22.