Extremely efficient gene transfection into lympho-hematopoietic cell lines by Epstein-Barr virus-based vectors

Journal of Immunological Methods

Volumn 204, Issue 2, 1997, Pages 143-151

  Mazda, O ^a   Satoh, E ^a   Yasutomi, K ^a   Imanishi, J ^a  

^a KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

Author keywords

Epstein Barr virus vector; Stable transfectant; Transfection; Transient expression

Indexed keywords

ARTICLE; CLONING VECTOR; CONTROLLED STUDY; DRUG RESISTANCE; EPSTEIN BARR VIRUS; GENETIC TRANSFECTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MARKER GENE; PLASMID; PRIORITY JOURNAL;

HUMAN HERPESVIRUS 4; MURINAE;

EID: 0031587032     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00036-7     Document Type: Article

References (20)

1. Gahn T.A., Sugden B. An EBNA-1-dependent enhancer acts from a distance of 10 kilobase pairs to increase expression of the Epstein-Barr virus LMP gene. J. Virol. 69:1995;2633-2636.
2. Germeraad W.T.V., Asami N., Fujimoto S., Mazda O., Katsura Y. Effective gene transduction into murine hematopoietic stem cells and long-lasting expression using a transwell coculture system. Blood. 84:1994;780-788.
3. Kaufman R.J. Vectors used for expression in mammalian cells. Methods Enzymol. 185:1990;487-495.
4. Kaufman R.J. Selection and coamplification of heterologous genes in mammalian cells. Methods Enzymol. 185:1990;537-566.
5. Kingston, R.E., 1991. Stable transfer of genes into mammalian cells. In: Coligan, J.E., Kruisbeek, A.M., Margulies, D.H., Shevach, E.M., Strober, W. (Eds.), Current Protocols in Immunology. Wiley, New York, pp. 10.17.1-10.17.7.
6. Kinoshita T., Miyata T., Inoue N., Takeda J. Expression cloning strategies for glycosylphosphatidylinositol-anchor biosynthesis enzymes and regulators. Methods Enzymol. 250:1995;547-560.
7. Kirchmaier A.L., Sugden B. Plasmid maintenance of derivatives of oriP of Epstein-Barr virus. J. Virol. 69:1995;1280-1283.
8. Lefkovits, I., Waldmann, H., 1979. Limiting Dilution Analysis of Cells in the Immune System. Cambridge University Press, Cambridge.
9. Mazda O., Watanabe Y., Katsura Y. Requirement of dendritic cells and B cells in the clonal deletion of Mls-reactive T cells in the thymus. J. Exp. Med. 173:1991;539-547.
10. Mazda O., Teshigawara K., Fujimoto S., Hattori N., Dou Y.M., Katsura Y. A reporter system using a flow cytometer to detect promoter/enhancer activity in lymphoid cell lines. J. Immunol. Methods. 169:1994;53-61.
11. Mazda O., Aiba Y., Hattori N., Li M., Fujimoto S., Davis M.M., Katsura Y. Abrogation of the allelic exclusion in a T cell receptor β chain gene transgenic mouse strain. Immunol. Invest. 24:1995;927-946.
12. Meneguzzi G., Bine'truy B., Grisoni M., Cuzin F. Plasmidial maintenance in rodent fibroblasts of a BPV1-pBR322 shuttle vector without immediately apparent oncogenic transformation of the recipient cells. EMBO J. 3:1984;365-371.
13. Middleton T., Sugden B. Retention of plasmid DNA in mammalian cells is enhanced by binding of the Epstein-Barr virus replication protein EBNA1. J. Virol. 68:1994;4067-4071.
14. Nakauchi H., Tagawa M., Nolan G.P., Herzenberg L.A. Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2. Nucleic Acids Res. 15:1987;4337-4347.
15. Rawlins D.R., Milman G., Hayward S.D., Hayward G.S. Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Cell. 42:1985;859-868.
16. Reisman D., Sugden B. Transactivation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1. Mol. Cell Biol. 6:1986;3838-3846.
17. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
18. Sugden B., Marsh K., Yates J. A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus. Mol. Cell Biol. 5:1985;410-413.
19. Takebe Y., Seiki M., Fujisawa J.I., Hoy P., Yokota K., Arai K.I., Yoshida M., Arai N. SR alpha promoter: An efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell Biol. 8:1988;466-472.
20. Yates J.L., Warren N., Sugden B. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells. Nature. 313:1985;812-815.