Gene expression profiles of normal proliferating and differentiating human intestinal epithelial cells: A comparison with the Caco-2 cell model

Journal of Cellular Biochemistry

Volumn 99, Issue 4, 2006, Pages 1175-1186

  Tremblay, Eric ^a,b   Auclair, Joëlle ^a,b   Delvin, Edgar ^a,c   Levy, Emile ^a,c   Ménard, Daniel ^a,b   Pshezhetsky, Alexey V ^c   Rivard, Nathalie ^a,b   Seidman, Ernest G ^a,c   Sinnett, Daniel ^c   Vachon, Pierre H ^a,b   Beaulieu, Jean François ^a,b  

^a CANADIAN INSTITUTES OF HEALTH RESEARCH   (Canada)

^b UNIVERSITY OF SHERBROOKE   (Canada)

^c UNIVERSITY OF MONTREAL   (Canada)

Author keywords

Genomics; Growth and differentiation; Human intestine

Indexed keywords

COMPLEMENTARY DNA;

ALGORITHM; ARTICLE; CARCINOGENESIS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRAIN CACO 2; CLUSTER ANALYSIS; COMPARATIVE STUDY; CONTROLLED STUDY; DNA MICROARRAY; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE TECHNOLOGY; HUMAN; HUMAN CELL; INTESTINE CRYPT; INTESTINE EPITHELIUM CELL; INTESTINE VILLUS; PHENOTYPE; PRIORITY JOURNAL; UPREGULATION;

EID: 33750542545     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.21015     Document Type: Article

References (57)

1. Babyatsky M, Podolsky D. 1999. Growth and development of the gastrointestinal tract. In: Yamada T, editor. Textbook of gastroenterology. Philadelphia: J.B. Lippincott. pp 547-584.
2. Basora N, Desloges N, Chang Q, Bouatrouss Y, Gosselin J, Poisson J, Sheppard D, Beaulieu JF. 1998. Expression of the alpha9beta1 integrin in human colonic epithelial cells: Resurgence of the fetal phenotype in a subset of colon cancers and adenocarcinoma cell lines. Int J Cancer 75:738-743.
3. Basora N, Herring-Gillam FE, Boudreau F, Perreault N, Pageot LP, Simoneau M, Bouatrouss Y, Beaulieu JF. 1999. Expression of functionally distinct variants of the beta(4)A integrin subunit in relation to the differentiation state in human intestinal cells. J Biol Chem 274:29819-29825.
4. Beaulieu JF. 1999. Integrins and human intestinal cell functions. Front Biosci 4:D310-D321.
5. Beaulieu JF, Quaroni A. 1991. Clonal analysis of sucrase-isomaltase expression in the human colon adenocarcinoma Caco-2 cells. Biochem J 280(Pt 3):599-608.
6. Behrens P, Brinkmann U, Wellmann A. 2003. CSE1L/CAS: Its role in proliferation and apoptosis. Apoptosis 8:39-44.
7. Benchimol S, Fuks A, Jothy S, Beauchemin N, Shirota K, Stanners CP. 1989. Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule. Cell 57:327-334.
8. Brinkmann U. 1998. CAS, the human homologue of the yeast chromosome-segregation gene CSE1, in proliferation, apoptosis, and cancer. Am J Hum Genet 62:509-513.
9. Cortez D, Glick G, Elledge SJ. 2004. Minichromosome maintenance proteins are direct targets of the ATM and ATR checkpoint kinases. Proc Natl Acad Sci USA 101:10078-10083.
10. Deschenes C, Vezina A, Beaulieu JF, Rivard N. 2001. Role of p27(Kip1) in human intestinal cell differentiation. Gastroenterology 120:423-438.
11. Desloges N, Basora N, Perreault N, Bouatrouss Y, Sheppard D, Beaulieu JF. 1998. Regulated expression of the integrin alpha9beta1 in the epithelium of the developing human gut and in intestinal cell lines: Relation with cell proliferation. J Cell Biochem 71:536-545.
12. Dydensborg AB, Herring E, Auclair J, Tremblay E, Beaulieu JF. 2006. Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon. Am J Physiol Gastrointest Liver Physiol 290:G1067-G1074.
13. Escaffit F, Perreault N, Jean D, Francoeur C, Herring E, Rancourt C, Rivard N, Vachon PH, Pare F, Boucher MP, Auclair J, Beaulieu JF. 2005. Repressed E-cadherin expression in the lower crypt of human small intestine: A cell marker of functional relevance. Exp Cell Res 302:206-220.
14. Evans GS, Flint N, Potten CS. 1994. Primary cultures for studies of cell regulation and physiology in intestinal epithelium. Annu Rev Physiol 56:399-417.
15. Fahlgren A, Baranov V, Frangsmyr L, Zoubir F, Hammarstrom ML, Hammarstrom S. 2003. Interferon-gamma tempers the expression of carcinoembryonic antigen family molecules in human colon cells: A possible role in innate mucosal defence. Scand J Immunol 58:628-641.
16. Fleet JC, Wang L, Vitek O, Craig BA, Edenberg HJ. 2003. Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation. Physiol Genomics 13:57-68.
17. Francoeur C, Escaffit F, Vachon PH, Beaulieu JF. 2004. Proinflammatory cytokines TNF-alpha and IFN-gamma alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 287:G592-G598.
18. Gilbreth M, Yang P, Bartholomeusz G, Pimental RA, Kansra S, Gadiraju R, Marcus S. 1998. Negative regulation of mitosis in fission yeast by the shk1 interacting protein skb1 and its human homolog, Skb1Hs. Proc Natl Acad Sci USA 95:14781-14786.
19. Gordon JI, Hermiston ML. 1994. Differentiation and self-renewal in the mouse gastrointestinal epithelium. Curr Opin Cell Biol 6:795-803.
20. Hammarstrom S, Baranov V. 2001. Is there a role for CEA in innate immunity in the colon? Trends Microbiol 9:119-125.
21. Harnois C, Demers MC, Bouchard V, Vallee K, Gagne D, Fujita N, Tsuruo T, Vezina A, Beaulieu JF, Cote A, Vachon PH. 2004. Human intestinal epithelial crypt cell survival and death: Complex modulation of Bcl-2 homologs by Fak, PI3-K/Akt-1, MEK/Erk, and p38 signaling pathways. J Cell Physiol 198:209-222.
22. Jumarie C, Malo C. 1991. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J Cell Physiol 149:24-33.
23. Lakshman M, Subramaniam V, Wong S, Jothy S. 2005. CD44 promotes resistance to apoptosis in murine colonic epithelium. J Cell Physiol 203:583-588.
24. Lane D, Cartier A, L'Esperance S, Cote M, Rancourt C, Piche A. 2004. Differential induction of apoptosis by tumor necrosis factor-related apoptosis-inducing ligand in human ovarian carcinoma cells. Gynecol Oncol 93:594-604.
25. Leblond CP. 1981. The life history of cells in renewing systems. Am J Anat 160:114-158.
26. Levy E, Mehran M, Seidman E. 1995. Caco-2 cells as a model for intestinal lipoprotein synthesis and secretion. FASEB J 9:626-635.
27. Levy E, Beaulieu JF, Delvin E, Seidman E, Yotov W, Basque JR, Menard D. 2000. Human crypt intestinal epithelial cells are capable of lipid production, apolipoprotein synthesis, and lipoprotein assembly. J Lipid Res 41:12-22.
28. Ménard D, Beaulieu J. 1994. Human intestinal brush border membrane hydrolases. In: Bkaily G, editor. Membrane physiopathology. Norwell, MA: Kluwer. pp 319-341.
29. Mariadason JM, Arango D, Corner GA, Aranes MJ, Hotchkiss KA, Yang W, Augenlicht LH. 2002. A gene expression profile that defines colon cell maturation in vitro. Cancer Res 62:4791-4804.
30. Mariadason JM, Nicholas C, L'Italien KE, Zhuang M, Smartt HJ, Heerdt BG, Yang W, Corner GA, Wilson AJ, Klampfer L, Arango D, Augenlicht LH. 2005. Gene expression profiling of intestinal epithelial cell maturation along the crypt-villus axis. Gastroenterology 128:1081-1088.
31. Montgomery RK, Mulberg AE, Grand RJ. 1999. Development of the human gastrointestinal tract: Twenty years of progress. Gastroenterology 116:702-731.
32. Nakagami K, Uchida T, Ohwada S, Koibuchi Y, Suda Y, Sekine T, Morishita Y. 1999. Increased choline kinase activity and elevated phosphocholine levels in human colon cancer. Jpn J Cancer Res 90:419-424.
33. Ni H, Dydensborg AB, Herring FE, Basora N, Gagne D, Vachon PH, Beaulieu JF. 2005. Upregulation of a functional form of the beta4 integrin subunit in colorectal cancers correlates with c-Myc expression. Oncogene 24:6820-6829.
34. Ono T, Kitaura H, Ugai H, Murata T, Yokoyama KK, Iguchi-Ariga SM, Ariga H. 2000. TOK-1, a novel p21Cip1-binding protein that cooperatively enhances p21-dependent inhibitory activity toward CDK2 kinase. J Biol Chem 275:31145-31154.
35. Otsuka M, Kato M, Yoshikawa T, Chen H, Brown EJ, Masuho Y, Omata M, Seki N. 2001. Differential expression of the L-plastin gene in human colorectal cancer progression and metastasis. Biochem Biophys Res Commun 289:876-881.
36. Pageot LP, Perreault N, Basora N, Francoeur C, Magny P, Beaulieu JF. 2000. Human cell models to study small intestinal functions: Recapitulation of the crypt-villus axis. Microsc Res Tech 49:394-406.
37. Perreault N, Beaulieu JF. 1996. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Exp Cell Res 224:354-364.
38. Perreault N, Beaulieu JF. 1998a. Primary cultures of fully differentiated and pure human intestinal epithelial cells. Exp Cell Res 245:34-42.
39. Perreault N, Herring-Gillam FE, Desloges N, Belanger I, Pageot LP, Beaulieu JF. 1998b. Epithelial vs. mesenchymal contribution to the extracellular matrix in the human intestine. Biochem Biophys Res Commun 248:121-126.
40. Pinto M, Robine-Leon S, Appay M, Kedinger M, Triadou N, Dussaulx E, Lacroix B, Simon-Assmann P, Haffen K, Fogh J, Zweibaum A. 1983. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell 47:323-330.
41. Podolsky DK. 1993. Regulation of intestinal epithelial proliferation: A few answers, many questions. Am J Physiol 264:G179-G186.
42. Potten CS, Loeffler M. 1990. Stem cells: Attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development 110:1001-1020.
43. Quaroni A, Beaulieu JF. 1997. Cell dynamics and differentiation of conditionally immortalized human intestinal epithelial cells. Gastroenterology 113:1198-1213.
44. Ramirez de Molina A, Rodriguez-Gonzalez A, Gutierrez R, Martinez-Pineiro L, Sanchez J, Bonilla F, Rosell R, Lacal J. 2002. Overexpression of choline kinase is a frequent feature in human tumor-derived cell lines and in lung, prostate, and colorectal human cancers. Biochem Biophys Res Commun 296:580-583.
45. Rozen S, Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365-386.
46. Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J. 2003. TM4: A free, open-source system for microarray data management and analysis. Biotechniques 34:374-378.
47. Seiden-Long IM, Brown KR, Shih W, Wigle DA, Radulovich N, Jurisica I, Tsao MS. 2005. Transcriptional targets of hepatocyte growth factor signaling and Ki-ras oncogene activation in colorectal cancer. Oncogene (advanced online publication).
48. Shupert WL, Chan WY. 1993. Pregnancy specific beta 1-glycoprotein in human intestine. Mol Cell Biochem 120:159-170.
49. Simoneau A, Herring-Gillam FE, Vachon PH, Perreault N, Basora N, Bouatrouss Y, Pageot LP, Zhou J, Beaulieu JF. 1998. Identification, distribution, and tissular origin of the alpha5(IV) and alpha6(IV) collagen chains in the developing human intestine. Dev Dyn 212:437-447.
50. Sugiyama Y, Farrow B, Murillo C, Li J, Watanabe H, Sugiyama K, Evers BM. 2005. Analysis of differential gene expression patterns in colon cancer and cancer stroma using microdissected tissues. Gastroenterology 128:480-486.
51. Tian JQ, Quaroni A. 1999. Involvement of p21(WAF1/Cip1) and p27(Kip1) in intestinal epithelial cell differentiation. Am J Physiol 276:C1245-C1258.
52. Vachon PH, Beaulieu JF. 1992. Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line. Gastroenterology 103:414-423.
53. Vachon PH, Beaulieu JF. 1995. Extracellular heterotrimeric laminin promotes differentiation in human enterocytes. Am J Physiol 268:G857-G867.
54. Watson AJ, Pritchard DM. 2000. Lessons from genetically engineered animal models. VII. Apoptosis in intestinal epithelium: Lessons from transgenic and knockout mice. Am J Physiol Gastrointest Liver Physiol 278:G1-5.
55. Whitehead R, Watson N. 1997. Gastrointestinal cell lines as a model for differentiation. In: Halter FWD, Wright NA, editors. The gut as a model in cell and molecular biology. Dordrecht: Kluwer. pp 275-290.
56. Wielenga VJ, Smits R, Korinek V, Smit L, Kielman M, Fodde R, Clevers H, Pals ST. 1999. Expression of CD44 in Ape and Tcf mutant mice implies regulation by the WNT pathway. Am J Pathol 154:515-523.
57. Zweibaum A, Chantret I. 1989. Human colon carcinoma cell lines as in vitro models for the study of intestinal cell differentiation. In: Smith MW, Sepulveda FV, editors. Adaptation and Development of the Intestinal Functions. Manchester: Manchester University Press, pp 103-112.