Deletion of DNA sequences of using a polymerase chain reaction based approach

Electronic Journal of Biotechnology

Volumn 9, Issue 5, 2006, Pages 604-609

  Pérez Pinera, Pablo ^a   Menéndez González, Manuel ^b   Vega, José Antonio ^a  

^a UNIVERSIDAD DE OVIEDO   (Spain)

^b HOSPITAL ÁLVAREZ BUYLLA   (Spain)

Author keywords

Deletion; Mutagenesis; Plasmid; Polymerase chain reaction

Indexed keywords

CELL CULTURE; DNA; LIGAMENTS; MUTAGENESIS; REACTION KINETICS;

PLASMIDS; POLYMERASE CHAIN REACTIONS; PROTEIN FUNCTION; RESTRICTION ENZYMES;

ENZYMES;

DNA FRAGMENT; PRIMER DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA SEQUENCE; GENE AMPLIFICATION; GENE DELETION; GENE MUTATION; HUMAN; HUMAN CELL; METHODOLOGY; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN FUNCTION;

EID: 33750504032     PISSN: 07173458     EISSN: 07173458     Source Type: Journal    
DOI: 10.2225/vol9-issue5-fulltext-10     Document Type: Article

References (14)

1. AILENBERG, Menachem; GOLDENBERG, Neil M. and SILVERMAN, Mel. Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5′ overhangs with run through stop DNA synthesis utilizing Ara-C. BMC Biotechnology, September 2005, vol. 5, p. 23.
2. ALLEMANDOU, Flore; NUSSBERGER, Jürg; BRUNNER, Hans R. and BRAKCH, Noureddine. Rapid site-directed mutagenesis using two-PCR-generated DNA fragments reproducing the plasmid template. Journal of Biomedicine and Biotechnology, 2003, vol. 3, p. 202-207.
3. CHIU, Joyce; MARCH, Paul E; LEE, Ryan and TILLETT, Daniel. Site-directed, ligase-independent mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h. Nucleic Acids Research, December 2004, vol. 32, no. 21, p. e174.
4. GAYTAN, Paul; YANEZ, Jorge; GRANDE, Ricardo; MORETT, Enrique and SOBERON, Xavier. Improving random mutagenesis by purification of the oligonucleotide variants. Combinational Chemistry and High Throughput Screening, September 2005, vol. 8, no. 6, p. 537-544.
5. KRAMER, Wilfried; DRUTSA, Valerji; JANSEN, Hans-W.; KRAMER, Barbara; PFLUGFELDER, Monika and FRITZL, Hans-Joachim. The gapped duplex DNA approach to oligonucleotide-directed mutation construction. Nucleic Acids Research, December 1984, vol. 12, no. 24, p. 9441-9456.
6. LACKS, Sanford A. Purification and properties of the complementary endonucleases Dpn I and Dpn II. Methods in Enzymology, 1980, vol. 65, no. 1, p. 138-146.
7. LANIO, Thomas and JELTSCH, Albert. PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type background. BioTechniques, December 1998, vol. 25, no. 6, p. 958-965.
8. LING, Mingfu F. and ROBINSON, Brian H. A one-step polymerase chain reaction site-directed mutagenesis method for large gene-cassettes with high efficiency, yield, and fidelity. Analytical Biochemistry, September 1995, vol. 230, no. 1, p.167-172.
9. NAGY, Zsolt B.; FELFÖLDI, Ferenc; TAMÁS, László and PUSKÁS, László G. A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product. Analytical Biochemistry, January 2004, vol. 324, no. 2, p. 301-303.
10. RABHI, Imen; GUEDEL, Naouel; CHOUK, Imen; ZERRIA, Khaled; BARBOUCHE, M. Ridha; DELLAGI, Koussay and FATHALLAH, Dahmani M. A novel simple and rapid PCR-based site-directed mutagenesis method. Molecular Biotechnology, January 2004, vol. 26, no. 1, p. 27-34.
11. SAIKI, Randall K.; SCHARF, Steven; FALOONA, Frank; MULLIS, Kary B.; HORN, Glenn T; ERLICH, Henry H. and ARNHEIM, Norman. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science, December 1985, vol. 230, no. 4732, p. 1350-1354.
12. SHENOY, Avinash R. and VISWESWARIAH, Sandhya S. Site-directed mutagenesis using a single mutagenic oligonucleotide and Dpn I digestion of template DNA. Analytical Biochemistry, August 2003, vol. 319, no. 2, p. 335-336.
13. WEI, Dongsheng; LI, Mingchun; ZHANG, Xinxin and XING, Laijun. An improvement of the site-directed mutagenesis method by combination of megaprimer, oneside PCR and Dpn I treatment. Analytical Biochemistry, August 2004, vol. 331, no. 2, p. 401-403.
14. WU, Wu; JIA, Zongchao; LIU, Ping; XIE, Zhigang and WEI, Qun. A novel PCR strategy for high-efficiency, automated site-directed mutagenesis. Nucleic Acids Research, August 2005, vol. 33, no. 13, p. e110.