Overexpression of the genes from thermophiles in Escherichia coli by high-temperature cultivation

Applied Microbiology and Biotechnology

Volumn 73, Issue 1, 2006, Pages 172-180

  Koma, Daisuke ^a   Sawai, Toshiya ^b   Harayama, Shigeaki ^a   Kino, Kuniki ^b  

^a NATIONAL INSTITUTE OF TECHNOLOGY AND EVALUATION   (Japan)

^b WASEDA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; HIGH TEMPERATURE EFFECTS;

PYROCOCCUS HORIKOSHII; SOLUBLE PROTEINS; THERMOPHILIC MICROORGANISMS;

GENES;

AMINOTRANSFERASE;

CLONE; CULTIVATION; GENE EXPRESSION; HIGH TEMPERATURE; PROTEIN; THERMOPHILIC BACTERIUM;

AEROPYRUM PERNIX; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HIGH TEMPERATURE PROCEDURES; MOLECULAR CLONING; NONHUMAN; PROTEIN AGGREGATION; PYROCOCCUS HORIKOSHII; SULFOLOBUS TOKODAII; THERMOPHILIC BACTERIUM;

AEROPYRUM; ARCHAEAL PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; PYROCOCCUS HORIKOSHII; RECOMBINANT PROTEINS; SULFOLOBUS; TEMPERATURE; TRANSAMINASES;

AEROPYRUM PERNIX; ESCHERICHIA COLI; PYROCOCCUS HORIKOSHII; SULFOLOBUS TOKODAII;

EID: 33750362182     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0448-9     Document Type: Article

References (24)

1. Andreotti G, Cubellis MV, Nitti G, Sannia G, Mai X, Adams MWW, Marino G (1995) An extremely thermostable aromatic aminotransferase from the hyperthermophilic archaeon Pyrococcus furiosus. Biochim Biophys Acta 1247:90-96
2. Andronopoulou E, Vorgias CE (2004) Isolation, cloning, and overexpression of a chitinase gene fragment from the hyperthermophilic archaeon Thermococcus chitonophagus: semi-denaturing purification of the recombinant peptide and investigation of its relation with other chitinases. Protein Expr Purif 35:264-271
3. Bowden GA, Georgiou G (1990) Folding and aggregation of beta-lactamase in the periplasmic space of Escherichia coli. J Biol Chem 265:16760-16766
4. Dabrowski S, Kiaer AB (2003) Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR. Protein Expr Purif 31:72-78
5. Grzybowska B, Szweda P, Synowiecki J (2004) Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme. Mol Biotechnol 26:101-110
6. Hannig G, Makrides SC (1998) Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol 16:54-60
7. Humphreys DP, Sehdev M, Chapman AP, Ganesh R, Smith BJ, King LM, Glover DJ, Reeks DG, Stephens PE (2000) High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5′ end of the coding sequence. Protein Expr Purif 20:252-264
8. Ideno A, Furutani M, Iwabuchi T, Iida T, Iba Y, Kurosawa Y, Sakuraba H, Ohshima T, Kawarabayashi Y, Maruyama T (2004) Expression of foreign proteins in Escherichia coli by fusing with an archaeal FK506 binding protein. Appl Microbiol Biotechnol 64:99-105
9. Ishida M, Oshima T, Yutani K (2002) Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus. FEMS Microbiol Lett 216:179-183
10. Jespers L, Sonveaux E, Fastrez J, Phanapoulos A, Davison J (1991) Overexpression of the phage lambda lysozyme cloned in Escherichia coli: use of a degenerative mixture of synthetic ribosome binding sites and increase of the protein stability in vivo. Protein Eng 4:485-492
11. Kim BC, Lee YH, Lee HS, Lee DW, Choe EA, Pyun YR (2002) Cloning, expression and characterization of L-arabinose isomerase from Thermotoga neapolitana: bioconversion of D-galactose to D-tagatose using the enzyme. FEMS Microbiol Lett 212:121-126
12. Kochhar S, Mouratou B, Christen P (2000) Amino acid analysis by high-performance liquid chromatography after derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's reagent). Methods Mol Biol 159:49-54
13. Kopetzki E, Schumacher G, Buckel P (1989) Control of formation of active soluble or inactive insoluble baker's yeast alpha-glucosidase PI in Escherichia coli by induction and growth conditions. Mol Gen Genet 216:149-155
14. Lu C, Erickson HP (1997) Expression in Escherichia coli of the thermostable DNA polymerase from Pyrococcus furiosus. Protein Expr Purif 11:179-184
15. Marfey P (1984) Determination of D-amino acids. II. Use of a bifunctional reagent, 1,5-difluoro-2,4-dinitrobenzene. Carlsberg Res Commun 49:591-596
16. Moreno R, Haro A, Castellanos A, Berenguer J (2005) High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus. Appl Environ Microbiol 71:591-593
17. Nishihara K, Kanemori M, Kitagawa M, Yanagi H, Yura T (1998) Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli. Appl Environ Microbiol 64:1694-1699
18. Novy R, Drott D, Yaeger K, Mierendorf R (2001) Overcoming the codon bias of E. coli for enhanced protein expression. Innovations 12:1-3
19. Ogasahara K, Nakamura M, Nakura S, Tsunasawa S, Kato I, Yoshimoto T, Yutani K (1998) The unusually slow unfolding rate causes the high stability of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus: equilibrium and kinetic studies of guanidine hydrochloride-induced unfolding and refolding. Biochemistry 37:17537-17544
20. Sambrock J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
21. Scalley ML, Baker D (1997) Protein folding kinetics exhibit an Arrhenius temperature dependence when corrected for the temperature dependence of protein stability. Proc Natl Acad Sci USA 94:10636-10640
22. Schofield LR, Patchett ML, Parker EJ (2004) Expression, purification, and characterization of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Pyrococcus furiosus. Protein Expr Purif 34:17-27
23. Word DE, Kengen SWM, van Der Oost J, de Vos WM (2000) Purification and characterization of the alanine aminotransferase from the hyperthermophilic archaeon Pyrococcus furiosus and its role in alanine production. J Bacteriol 182:2559-2566
24. Yu L, Deng K-P, Yu C-A (1995) Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrome c reductase. J Biol Chem 270:25634-25638