A rapid method for differentiating Saccharomyces sensu stricto strains from other yeast species in an enological environment

FEMS Microbiology Letters

Volumn 264, Issue 2, 2006, Pages 168-173

  Nardi, Tiziana ^a   Carlot, Milena ^a   De Bortoli, Elena ^a   Corich, Viviana ^a   Giacomini, Alessio ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

Enological yeasts; Multiplex PCR; Ribosomal DNA sequences; Saccharomyces sensu stricto; Strain selection; Taxonomy

Indexed keywords

FUNGAL RNA; RIBOSOME RNA;

ARTICLE; CONTROLLED STUDY; FUNGAL STRAIN; FUNGUS ISOLATION; GENE AMPLIFICATION; GENE INSERTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SACCHAROMYCES; SACCHAROMYCES SENSU STRICTO; SPECIES DIFFERENTIATION;

DNA PRIMERS; DNA, RIBOSOMAL; MYCOLOGICAL TYPING TECHNIQUES; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL; RNA, RIBOSOMAL, 18S; SACCHAROMYCES; WINE;

SACCHAROMYCES;

EID: 33750299182     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2006.00450.x     Document Type: Article

References (15)

1. Cavazza A, Grando MS & Zini C (1992) Rilevazione della flora microbica in mosti e vini. Vignevini 9: 17-20.
2. Green SR & Gray PP (1950) A differential procedure applicable to bacteriological investigation in brewing. Wallerstein Lab Comm 13: 357-366.
3. Kurtzman CP (2003) Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FEMS Yeast Res 4: 233-245.
4. Kurtzman CP & Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73: 331-371.
5. Kurtzman CP & Robnett CJ (2003) Phylogenetic relationships among yeasts of the 'Saccharomyces complex' determined from multigene sequence analyses. FEMS Yeast Res 3: 417-432.
6. Manzano M, Cocolin L, Longo B & Comi G (2004) PCR-DGGE differentiation of strains of Saccharomyces sensu stricto. Antonie van Leeuwenhoek 85: 23-27.
7. Niemann S, Dammann-Kalinowski T, Nagel A, Pühler A & Selbitschka W (1999) Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S. meliloti employing PCR primers derived from an ERIC-PCR fragment. Arch Microbiol 172: 22-30.
8. O'Donnel K (1993) Fusarium and its near relatives. The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics (Reynolds DR & Taylor JW, eds), pp. 225-233. CAB International, Wallingford, UK.
9. Olsen GJ, Lane DJ, Giovannoni SJ, Pace NR & Stahl DA (1986) Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol 40: 337-365.
10. Pallmann CL, Brown JA, Olineka TL, Cocolin L, Mills DA & Bisson L (2001) Use of WL medium to profile native flora fermentations. Am J Enol Vitic 52: 198-203.
11. Rainieri S, Zambonelli C & Kaneko Y (2003) Saccharomyces sensu stricto: systematics, genetic diversity and evolution. J Biosci Bioeng 1: 1-9.
12. Scorzetti G, Fell JW, Fonseca A & Statzell-Tallman A (2002) Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2: 495-517.
13. Thompson JD, Higgins DG & Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680.
14. van der Walt JP (1970) Saccharomyces Meyen emend Reess. The Yeasts: ATaxonomic Study (Lodder J, ed), pp. 555-718. Elsevier, Amsterdam, the Netherlands.
15. White TJ, Bruns T, Lee S & Taylor J (1990) PCR protocols. A guide to methods and applications. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics (Innis MA, Gelfand DH, Sninsky JJ & White TJ, eds), pp. 315-322. Academic Press, San Diego, CA.