Confocal quantification of cis-regulatory reporter gene expression in living sea urchin

Developmental Biology

Volumn 299, Issue 2, 2006, Pages 543-550

  Damle, Sagar ^a   Hanser, Bridget ^a,b   Davidson, Eric H ^a   Fraser, Scott E ^a,b,c  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^c California Institute of Technology   (United States)

Author keywords

Confocal laser scanning microscopy; GFP; Sea urchin cis regulation

Indexed keywords

DEXTRAN DERIVATIVE; DNA; GREEN FLUORESCENT PROTEIN;

ACCURACY; ANIMAL TISSUE; ARTICLE; BIOLOGY; BONE CELL; BONE DEVELOPMENT; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DEVELOPMENTAL STAGE; EGG; EMBRYO; EMBRYO DEVELOPMENT; FLUORESCENCE; GASTRULA; GENE CONSTRUCT; GENE EXPRESSION; GENE TRANSFER; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE GENETICS; REPORTER GENE; SEA URCHIN; SIGNAL PROCESSING; STANDARD; STEREOISOMERISM; TISSUE SECTION; VALIDATION PROCESS;

ECHINOIDEA;

EID: 33750297649     PISSN: 00121606     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ydbio.2006.06.016     Document Type: Article

References (20)

1. Abramoff M.D., Magalhaes P.J., and Ram S.J. Image processing with ImageJ. Biophoton. Int. 11 (2004) 36-42
2. Arnone M.I., Dmochowski I.J., and Gache C. Using reporter genes to study cis-regulatory elements. Methods Cell Biol. 74 (2004) 621-652
3. Chalfie M., Tu Y., Euskirchen G., Ward W.W., and Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-805
4. Croce J., Lhomond G., Lozano J.C., and Gache C. ske-T, a T-box gene expressed in the skeletogenic mesenchyme lineage of the sea urchin embryo. Mech. Dev. 107 (2001) 159-162
5. Davidson E.H. Gene Activity in Early Development. 3rd ed. (1986), Academic Press, Orlando, FL 126-193
6. Davidson E.H., et al. A genomic regulatory network for development. Science 295 (2002) 1669-1678
7. Dmochowski I.J., Dmochowski J.E., Oliveri P., Davidson E.H., and Fraser S.E. Quantitative imaging of cis-regulatory reporters in living embryos. Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 12895-12900
8. Forouhar A.S., Liebling M., Hickerson A., Nasiraei-Moghaddam A., Tsai H.J., Hove J.R., Fraser S.E., Dickinson M.E., and Gharib M. The embryonic vertebrate heart tube is a dynamic suction pump. Science 312 (2006) 751-753
9. Hodor P.G., and Ettensohn C.A. The dynamics and regulation of mesenchymal cell fusion in the sea urchin embryo. Dev. Biol. 199 (1998) 111-124
10. Istrail S., and Davidson E.H. Logic functions of the genomic cis-regulatory code. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 4954-4959
11. Lee P.Y., and Davidson E.H. Expression of Spgatae, the Strongylocentrotus purpuratus ortholog of vertebrate GATA4/5/6 factors. Gene Expr. Patterns 5 (2004) 161-165
12. McMahon A.P., Flytzanis C.N., Hough-Evans B.R., Katula K.S., Britten R.J., and Davidson E.H. Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis. Dev. Biol. 108 (1985) 420-430
13. Oliveri P., and Davidson E.H. Gene regulatory network controlling embryonic specification in the sea urchin. Curr. Opin. Genet. Dev. 14 (2004) 351-360
14. Revilla-i-Domingo R., Minokawa T., and Davidson E.H. R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres. Dev. Biol. 274 (2004) 438-451
15. Wang D.G., Britten R.J., and Davidson E.H. Maternal and embryonic provenance of a sea urchin embryo transcription factor, SpZ12-1. Mol. Mar. Biol. Biotechnol. 4 (1995) 148-153
16. Yu D., Ellis H.M., Lee E.C., Jenkins N.A., Copeland N.G., and Court D.L. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 5978-5983
17. Yuh C.H., Bolouri H., and Davidson E.H. Genomic cis-regulatory logic: experimental and computational analysis of a sea urchin gene. Science 279 (1998) 1896-1902
18. Yuh C.H., Bolouri H., and Davidson E.H. Cis-regulatory logic in the endo16 gene: switching from a specification to a differentiation mode of control. Development 128 (2001) 617-629
19. Yuh C.H., Dorman E.R., Howard M.L., and Davidson E.H. An otx cis-regulatory module: a key node in the sea urchin endomesoderm gene regulatory network. Dev. Biol. 269 (2004) 536-551
20. Yuh C.H., Dorman E.R., and Davidson E.H. Brn1/2/4, the predicted midgut regulator of the endo16 gene of the sea urchin embryo. Dev. Biol. 281 (2005) 286-298