Bioremediation of oil-contaminated soil using rhizobacteria and plants

Korean Journal of Microbiology and Biotechnology

Volumn 34, Issue 3, 2006, Pages 185-195

  Kim, Ji Young ^a   Cho, Kyung Suk ^a  

^a Ewha Womans University   (South Korea)

Author keywords

Petroleum hydrocarbon; Plant assisted bioremediation; Rhizobacteria; Rhizodegradation; Soil pollution

Indexed keywords

ALKANE DERIVATIVE; ALKYNE DERIVATIVE; HEAVY METAL; PETROLEUM DERIVATIVE; POLYCHLORINATED BIPHENYL; POLYCYCLIC AROMATIC HYDROCARBON; POLYCYCLIC AROMATIC HYDROCARBON DERIVATIVE; TETRACHLOROETHYLENE; TRICHLOROETHYLENE;

ALCALIGENES; ALFALFA; BACILLUS; BIOREMEDIATION; CHEMICAL STRUCTURE; CORN; ECOSYSTEM RESTORATION; MICROBIAL DEGRADATION; MICROBIAL POPULATION DYNAMICS; MICROORGANISM; NONHUMAN; PERENNIAL RYEGRASS; PHYTOREMEDIATION; PLANT; PLANT MICROORGANISM INTERACTION; POLLUTANT; PSEUDOMONAS; REVIEW; SOIL MICROFLORA; SOIL POLLUTION; SPECIES IDENTIFICATION; TALL FESCUE;

ALCALIGENES; FESTUCA ARUNDINACEA; LOLIUM; MEDICAGO SATIVA; PSEUDOMONAS; RHIZOBIALES; ZEA MAYS;

EID: 33750044064     PISSN: 1598642X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (105)

1. J. Kor. Geoenviron. Soc. 2: 10-19.
2. Al-Ghazawi, Z., I. Saadoun, and A. Al-Shak'ah. 2005. Selection of bacteria and plant seeds for potential use in the remediation of diesel contaminated soils. J. Basic Microbiol. 45: 251-256.
3. Al-Sharidah, A., A. Richardt, J. R. Golecki, R. Dierstein, and M. H. Tadros. 2000. Isolation and characterization of two hydrocarbon-degrading Bacillus subtilis strains from oil contaminated soil of Kuwait. Microbiol Res. 155: 157-164.
4. Andreoni, V., L. Cavalca, M. A. Rao, G. Nocerino, S. Bernasconi, E. Dell'Amico, M. Colombo, and L. Gianfreda. 2004. Bacterial communities and enzyme activities of PAHs polluted soils. Chemosphere 57: 401-412.
5. Aprill, W. and R. C. Sims. 1990. Evaluation of the use of prairie grasses for stimulating polycyclic aromatic hydrocarbon treatment in soil. Chemosphere 20: 253-265.
6. ATSDR. 1999. Public health statement for total petroleum hydrocarbons (TPH).
7. Banks, M. K., P. Kulakow, A. P. Schwab, Z. Chen, and K. Rathbone. 2003. Degradation of crude oil in the rhizosphere of sorghum bicolor. Int. J. Phytoremediation 5: 225-234
8. Barac, T., S. Taghavi, B. Borremans, A. Provoost, L. Oeyen, J. V. Colpaert, J. Vangronsveld, and D. van der Lelie. 2004. Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants. Nat. Biotechnol. 22: 583-588.
9. Baudoin, E., E. Benizri, and A. Guckert. 2003. Inpact of artificial root exudates on the bacterial community structure in bulk soil and maize rhizosphere. Soil Biol. Biochem. 35: 1183-1192.
10. Bento, F. M., F. A. O. Camargo, B. C. Okeke, and W. T. Frankenberger. 2005. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. Bioresour. Technol. 96: 1049-1055.
11. Bollag, J. M., T. Mertz, and L. Otjen. 1994. Role of microorganisms in soil bioremediation. p. 2-10. In T. A Anderson and J. R. Coats (ed.), Bioremediation Through Rhizosphere Technology, vol. 563, American Chemical Society: Washington, D.C. ACS Symposium Series, U.S.A.
12. Chang, J. H., S. K. Rhee, Y. K. Chang, and H. N. Chang. 1998. Desulfurization of diesel oils by a newly isolated dibenzothiophene-degrading Nocardia sp. strain CYKS2. Biotechnol Prog. 14: 851-855.
13. Chen, Y. C., M. K. Banks, and A. P. Schwab. 2003. Pyrene degradation in the rhizosphere of tall fescue (Festuca arundinacea) and switchgrass (Panicum virgatum L.). Environ. Sci. Technol. 37: 5778-5782.
14. Chin-A-Woeng, T. F. C., G. V. Bloemberg, A. J. van der Bij, K. M. G. M. van der Drift, J. Schripsema, B. Kroon, R. J. Scheffer, C. Keel, P. A. H. M. Bakker, H. V. Tichy, F. J. de Bruijn, J. E. Thomas-Oates, and B. J. J. Lugtenberg. 1998. Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root caused by Fusarium oxysporum f. sp. radicis-lycopersici. Mol. Plant Microbe Interact. 11: 1069-1077.
15. Cohen, M. F., J. Williams, and H. Yamasaki. 2002. Biodegradation of diesel fuel by an Azolla-derived bacterial consortium. J. Environ. Sci. Health Part A Toxic-Hazard Subst. Environ. Eng. 37: 1593-1606.
16. Committee on In Situ Bioremediation, Water Science and Technology Board, Commission on Engineering and Technical Systems, and National Research Council. 1993. In Situ Bioremediation: When Does It Work? National Academy Press, Washington, D.C., U.S.A.
17. Cunningham, S. D., T. A. Anderson, A. P. Schwab, and F. C. Hsu. 1996. Phytoremediation of soils contaminated with organic pollutants. Adv. Agron. 56: 55-114.
18. Dandie, C. E., S. M. Thomas, R. H. Bentham, and N. C. McClure. 2004. Physiological characterization of Mycobacterium sp. strain 1B isolated from a bacterial culture able to degrade high-molecular-weight polycyclic aromatic hydrocarbons. J. Appl. Microbiol. 97: 246-255.
19. De Wever, H. and H. Verachten. 1997. Biodegradation and toxicity of benzothiazoles. Wat. Res. 31: 2673-2684.
20. Dore, S. Y., Q. E. Clancy, S. M. Rylee, and C. F. Kulpa, Jr. 2003. Naphthalene-utilizing and mercury-resistant bacteria isolated from an acidic environment. Appl. Microbiol. Biotechnol. 63: 194-199.
21. Durmishidze, S. V. 1977. Metabolism of certain air-polluting organic compounds in plant (review). Appl. Biochem. Microbiol. 13: 646-653
22. Edwards, N. T., B. M. Ross-Todd, and E. G. Garver. 1982. Uptake and metabolism of 14C anthracene by soybean (Glycine max). Environ. Exp. Bot. 22: 349-357.
23. Edwards, N. T. 1988. Assimilation and metabolism of polycyclic aromatic hydrocarbons by vegetation - An approach to this controversial issue and suggestions for future research, p. 211-229. In M. Cook and A.J. Dennis (eds.), Polycyclic aromatic hydrocarbons: A decade of progress. 10th Int. Symp. Battelle Press, Columbus, Ohio, U.S.A.
24. Escalante-Espinosa, E., M. E. Gallegos-Martinez, E. Favela-Torre, and M. Gutierrez-Rojas. 2005. Improvement of the hydrocarbon phytoremediation rate by Cyperus laxus Lam. Inoculated with a microbial consortium in a model system. Chemosphere 59: 405-413.
25. Eweis, J. B., S. J. Ergas, D. P. Y. Chang, and E. D. Schroeder. 1998. Bioremediation principles. McGraq-Hill, Inc.: Toronto, Canada.
26. Farrell, R. E., C. M. Frick, and J. J. Germida. 2000. A database of plants that play a role in the phytoremediation of petroleum hydrocarbons, p. 29-40. Proceedings of the Second Phytoremediation Technical Seminar, Environment Canada, Ottawa, Canada.
27. Frick, C. M., R. E. Farrell, and J. J. Germida. 1999. Assessment of phytoremediation as an In-Situ technique for cleaning oil-contaminated sites. Petroleum Technology Alliance of Canada, Calgary, Canada.
28. Gunther, T., U. Dornberger, and W. Pritsche. 1996. Effects of ryegrass on biodegradation of hydrocarbons in soil. Chemosphere 33: 203-215.
29. Hamnann, C., J. Hegemann, and A. Hildebrandt. 1999. Detection of polycyclic aromatic hydrocarbon degradation genes in different soil bacteria by polymerase chain reaction and DNA hybridization. FEMS Microbiol. Lett. 173: 255-263.
30. Hegde, R. S. and J. S. Fletcher. 1997. Influence of plant growth stage and season on the release of root phenolics by mulberry as related to development of phytoremediation technology. Chemosphere 32: 2471-2479.
31. Huang, X. D., Y. El-Alawi, J. Gurska, B. R. Glick, and B. M. A. Greenberg. A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons (TPHs) from soils. Microchem. J. 81: 139-147.
32. Hutchinson, S. L., A. P. Schwab, and M. K. Banks. 2003. Biodegradation of petroleum hydrocarbons in the rhizosphere. p. 355-386. In S. C. McCutcheon, and J. L. Schnoor (eds.), Transformation and Control of Contaminants, Wiley, New York, U.S A.
33. Hynes, R. K., R. E. Farrell, and J. J. Germida. 2004. Plant-assisted degradation of phenanthrene as assessed by solid-phase microextraction (SPME). Int. J Phytoremediation 6: 253-268.
34. Ilori, M. O. and D. I. Amund. 2000. Degradation of anthracene by bacteria isolated from oil polluted tropical soils. Z Naturforsch [C]. 55: 890-897.
35. Jirausch, M., O. Asperger, and H. P. Kleber. 1986. Alcohol oxidation by Acinetobacter calcoaceticus EB 104-a n-alkane-utilizing and cytochrome P-450-producing strain. J. Basic Microbiol. 26: 351-357.
36. Jordahl, J. L., L. Foster, J. L. Schnoor, and P. J. J. Alvarez. 1997. Effect of hybrid poplar trees on microbial populations important to hazardous waste bioremediation. Environ. Toxicol. Chem. 16: 1318-1321.
37. Kaksonen, A. H., M. M. Jussila, K. Lindstrom, and L. Suominen. 2006. Rhizosphere effect of Galega orientalis in oil-contaminated soil. Soil Biol. Biochem. 38: 817-827.
38. Kanaly, R. A., R. Bartha, K. Watanabe, and S. Harayama. 2000. Rapid mineralization of benzo[a]pyrene by a microbial consortium growing on diesel fuel. Appl. Environ. Microbiol. 66: 4205-4211.
39. Karthikeyan, R., K. R. Mankin, L. C. Davis, and L. E. Erickson. 2003. Fate and transport of jet fuel (JP-8) in soils with selected plants. Int. J Phytoremediation 5: 281-292.
40. Ke, L., W. Q. Wong, T. W. Wong, Y. S. Wong, and N. F. Tam. 2003. Removal of pyrene from contaminated sediments by mangrove microcosms. Chemosphere 51: 25-34.
41. Khomiakova, D. V., I. V. Botvinko, and A. I. Netrusov. 2003. Isolation of hydrocarbon-oxidizing psychrophilic bacteria from oil-polluted soils. Prikl Biokhim Mikrobiol. 39: 661-664.
42. King, J. M. H., P. M. DiGrazia, B. Applegate, F. Larimer, and G. S. Sayler. 1990. Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation. Science 249: 778-781.
43. Kingsley, M. T., J. K. Fredrickson, F. B. Metting, and R. J. Seidler. 1994. Environmental restoration using plant-microbe bioaugmentation. p. 287-292 In R. E. Hinchee, A. Leeson, L. Semprini, and S. K. Ong (eds.), Bioremediatin of Chlorinated and Polyaromatic Hydrocarbon Compounds, Lewis Publishers, Boca Raton, FL, U.S.A.
44. Kuiper, I., G. V. Bioemberg, and B. J. J. Lugtenberg. 2001. Selection of a plant-bacterium pair as a novel tool for rhizostimulation of polycyclic aromatic hydrocarbon-degrading bacteria. Mol. Plant Microbe Interact. 14: 1197-1205.
45. Kuiper, I., E. L. Lagendijk, F. V. Bioemberg, and B. J. J. Lugtenberg. 2004. Rhizoremediation: A beneficial plant-microbe interaction (review). Mol. Plant Microbe Interact. 17: 6-15.
46. Lalande, T. L., H. D. Skipper, D. C. Wolf, C. M. Reynolds, D. L. Freedman, B. W. Pinkerton, P. G. Hartel, and L. W. Grimes. 2003. Phytoremediation of pyrene in a cecil soil under field conditions. Int. J Phytoremediation 5: 1-12.
47. Leigh, M. B., J. S. Fletcher, X. Fu, and F. J. Schmitz. 2000. Root turnover: an important source of microbial substances in rhizosphere remediation of recalcitrant contaminants. Environ. Sci. Technol. 36: 1579-1583.
48. Lindstrom, J. E., R. C. Prince, J. C. Clark, M. J. Grossman, T. R. Yeager, J. F. Braddock, and E. J. Brown. 1991. Microbial populations and hydrocarbon biodegradation potentials in fertilized shoreline sediments affected by the T/V Exxon Valdez oil spill. Appl. Environ. Microbiol. 57: 2514-2522.
49. Liste, H. J. and M. Alexander. 2000. Plant-promoted pyrene degradation in soil. Chemosphere 40: 7-10.
50. Liste, H. H. and D. Felgentreu. 2006. Crop growth, culturable bacteria, and degradation of petrol hydrocarbons (PHCs) in a long-term contaminated field soil. Appl. Soil Ecol. 31: 43-52.
51. Liste, H. H. and I. Prutz. 2006. Plant performance, dioxygenase- expressing rhizosphere bacteria, and biodegradation of seathered hydrocarbons in contaminated soil. Chemosphere 62: 1411-1420.
52. Lugtenberg, B. J. J. and L. A. de Weger. 1992. Plant root colonization by Pseudomonas spp.. p. 13-19. In E. Galli, S. Silver, and B. Witholt (eds.), Pseudomonas: Molecular Biology and Biotechnology, des. Am. Soc. Microbiol., Washington, D.C., U.S.A.
53. Lynch, J. M. and J. M. Whipps. 1990. Substrate flow in the rhizosphere. Plant Soil 129: 1-10.
54. Macek, T., M. Mackova, and J. Kas. 2000. Exploitation of plants for the removal of organics in environmental remediation (research review paper). Biotechnol. Adv. 18: 23-34.
55. McLean, L., J. Ireland, S. Hohn, and L. Herman. 1999. Preliminary report and design of using jack pines for the phytoremediation of diesel-contaminated soils in Northern Saskatchewan. p. 159-167. Proceedings of the Phytoremediation Technical Seminar, Calgary, Alberta, Ottawa, Canada.
56. Mehmannavaz, R., S. O. Prasher, and D. Ahmad. 2002. Rhizospheric effects of alfalfa on biotransformation of polychlorinated biphenyls in a contaminated soil augmented with Sinorhizobium melioti. Process Biochem. 37: 955-963.
57. Menezes Bento, F., F. A. de Oliveira Camargo, B. C. Okeke, and W. T. Frankenberger, Jr. 2005. Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiol. Res. 160: 249-255.
58. Merkl, N., R. Schultze-Kraft, andx M. Schultze-Kraf. 2005. Effect of the tropical grass Brachiaria brizantha (Hochst. Ex A. Rich.) Stapf on microbial population and activity in petroleum-contaminated soil. Microbiol. Res. 161: 80-91.
59. Mikolasch, A., E. Hammer, and F. Schauer. 2003. Synthesis of Imidazol-2-yl Amino Acids by Using Cells from Alkane-Oxidizing Bacteria Appl. Environ. Microbiol. 69: 1670-1679.
60. Monferran, M. V., J. R. Echenique, and D. A. Wunderlin. 2005. Degradation of chlorobenzenes by a strain of Acidovorax avenae isolated from a polluted aquifer. Chemosphere 61: 98-106.
61. Morgan, P. and R. J. Watkinson. 1994. Biodegradation of components of petroleum, p. 1-31. In C. Ratledge (ed.) Biochemistry of Microbial Degradation, Kluwer Academic Publisher, Dordrecht, Netherlands.
62. Muratova, A., T. Hubner, N. Narula, H. Wand, L. Turkovskaya, P. Kuschk, R. Jahn, and W. Merbach. 2003. Rhizosphere microflora of plants used for the phytoremediation of bitumen-contaminated soil. Microbiol. Res. 158: 151-161.
63. Nichols, T. D., E. C. Wolf, H. B. Rogers, C. A. Beyrouty, and C. M. Beyrouty. 1997. Rhizosphere microbial populations in contaminated soils. Water Air Soil Pollut. 95: 165-178.
64. Palmroth, M. R., J. Pichtel, and J. A. Puhakka. 2002. Phytoremediation of subarctic soil contaminated with diesel fuel. Bioresour Technol. 84: 221-228.
65. Palmroth, M. R., U. Munster, J. Pichtel, and J. A. Puhakka. 2005. Metaboilc responses of microbiota to diesel fuel addition in vegetated soil. Biodegradation. 16: 91-101.
66. Pepi, M., A. Minacci, F. Di cello, F. Baldi, and R. Fani. 2003. Logn-term analysis of diesel fuel consumption in a co-culture of Acinetobacter venetianus, Pseudomonas putida and Alcaligenes faecalis. Antonie Van Leeuwenhoek. 83: 3-9.
67. Pilon-Smits, E. 2005. Phytoremediation. Annu Rev Plant Biol. 56: 15-39.
68. Plaza, G. A., K. Ulfig, and R. L. Brigmon. 2005. Surface active properties of bacterial strains isolated from petroleum hydrocarbon- bioremediated soil. Pol. J. Microbiol. 54: 161-167.
69. Pradhan, S. P., J. R. Conrad, J. R. Paterek, and V. J. Srivastava. 1998. Potential of phytoremediation for treatment of PAHs in soil at MGP sites. J Soil Contam. 7: 467-480.
70. Radwan, S. S., H. Al-Awadhi, N. A. Sorkhoh, and I. M. El-Nemr. 1998. Rhizospheric hydrocarbon-utilizing microorganisms as potential contributors to phytoremediation for the oily Kuwaiti desert. Microbiol. Res. 153: 247-251.
71. Radwan, S., N. Dashti, and I. El-Nemr. 2005. Engancing the growth of Vicia faba plants by microbial inoculation to improve their phytoremediation potential for oily desert area. Int. J. Phytoremediation. 1: 19-32
72. Rahman, K. S., T. Rahman, P. Lakshmanaperumalsamy, and I. M. Banat. 2002. Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. J. Basic Microbiol. 42: 284-291.
73. Reilley, K. A., M. K. Banks, and A. P. Schwab. 1996. Organic chemicals in the environment: dissipation of polycyclic aromatic hydrocarbons in the rhizosphere. J. Environ. Qual. 25: 212-219.
74. Rentz, A. J., B. Chapman, P. J. J. Alvarez, and J. L. Schnoor. 2003. Stimulation of hybrid poplar grwth in petroleum contaminated soils through oxygen addition and soil nutrient amendments. Int J Phytoremediation 5: 57-72.
75. Rentz, J. A., P. J. J. Alvarez, and J. L. Schnoor. 2005. Benzo[a]pyrene co-metabolism in the presence of plant root extracts and exudates: Implications for phytoremediation. Environ. Pollut. 136: 477-484.
76. Reynold, C. M. and D. C. Wolf. 1999. Microbial based strategies for assessing rhizosphere-enhanced phytoremediation. p. 125-135. Proceedings of the Phytoremediation Technical Seminar, Calgary, Alberta, Ottawa, Canada.
77. Rojas-Avelizapa, N. G., R. Rodriguez-Vazquez, J. Martinez-Cruz, F. Esparza-Garcia, A. Montes de Oca-Garcia, E. Rios-Leal, and G. Fernandez-Villagomez, 1999. Isolation and characterization of bacteria degrading polychlorinated biphenyls from transformer oil. Folia Microbiol (Praha). 44: 317-321.
78. Ronchel, M. C. and J. L. Ramos. 2001. Dual system to reinforce biological containment of recombinant bacteria designed for rhizoremediation. Appl. Environ. Microbiol. 67: 2649-2656.
79. Ruberto, L., S. C. Vazquez, W. P. Mac Cormack. 2003. Effectiveness of the natural bacterial flora, biostimulation and bioqugmentation on the bioremediation of a hydrocarbon contaminated Actarctic soil. Int. Biodeterior. Biodeg. 52: 115-125.
80. Schwab, A. P., M. K. Banks, and M. Arunachalam. 1995. Biodegradation of polycyclic aromatic hydrocarbons in rhizosphere soil. p. 23-29. In R.E. Hinchee, D.B. Anderson, and R.E. Hoeppel (eds.), Bioremediation of Recalcitrant Organics, Battelle Press, Columbus, U.S.A.
81. Shao, Z. Z., Y. Xu, Y. F. Ma, and Q. Guo. 2004. Isolation and identification of two marine bacteria with hydrocarbon-biodegradation activity. Huan Jing Ke Xue. 25: 133-137.
82. Shcherbakova, V. A., K. S. Laurinavichus, A. M. Lysenko, N. E. Suzina, and V. K. Akimenko. 2003. Methanogenic sarcina from an anaerobic microbial community degrading p-toluene sulfonate. Mikrobiologiia 72: 547-553.
83. Shim, H., S. Chauhan, D. Ryoo, K. Bowers, S. M. Thomas, K. A. Canada, J. G. Burken, and T. K. Wood. 2000. Rhozosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria. Appl. Environ. Microbiol. 66: 4673-4678.
84. Siciliano, S. D. and J. J. Germida. 1998. Mechanisms of phytoremediation: biochemical and ecological interactions between plants and bacteria. Environ. Rev. 6: 65-79
85. Siciliano, S. D., N. Fortin, A. Mihoc, G. Wisse, S. Labelle, K. Beaumier, D. Ouellette, R. Roy, L. G. Whyte, M. K. Banks, P. Schwab, K. Lee, and C. W. Greer. 2001. Selection of specific endophytic bacterial genotypes by plants in response to soil contamination. Appl. Environ. Microbiol. 67: 2469-2475.
86. Singer, A. C., D. Smith, W. A. Jury, H. Khoiviet, and D. E. Crowley. 2003. Impact of the plant rhizosphere and augmentation on remediation of polychlorinated biphenyl contaminated soil. Environ. Toxicol. Chem. 22: 1998-2004.
87. Smith, R. M. 1994. The Physiology of aromatic hydrocarbon degrading bacteria, p 365-367. In C. Ratledge (ed.), Biochemistry of Microbial Degradation, Kluwer Academic Publisher, Dordrecht. Netherlands.
88. Sung, J., C. L. Munster, R. Rhykerd, M. C. Drew, and M. Y. Corapcioglu. 2003. The use of vegetation to remediate soil freshly contaminated recalcitrant contaminants. Wat. Res. 37: 2408-2418.
89. Sutherland, J. B. 1992. Detoxification of polycyclic aromatic hydrocarbons by fungi. J. Ind. Microbiol. 9: 53-62.
90. Tesar, M., T. G. Reichenauer, and A. Sessitsch. 2002. Bacterial rhizosphere populations of black poplar and herbal plants to be used for phytoremediation of diesel fuel. Soil Biol. Biochem. 34: 1883-1892.
91. Tischer, S. and T. Hubner. 2002. Model trials for phytoremediation of hydrocarbon-contaminated sites by the use of different plant species. Int. J. Phytoremediation 4: 187-203.
92. Totevova, S., M. Prouza, J. Burkhard, K. Demnerova, and V. Brenner. 2002. Characterization of polychlorinated biphenyl-degrading bacteria isolated from contaminated sites in Czechia. Folia Microbiol (Praga). 47: 247-254.
93. Ueno, A., M. Hasanuzzaman, I. Yumoto, and H. Okuyama. 2006. Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms. Curr. Microbiol. 52: 182-185.
94. USEPA, 1999. Phytoremediation resource guide. EPA/52/B-99/0.
95. USEPA, 2004. Abstracts of remediation case studies, Volume 8, EPA/542/R-04/012.
96. Vervaeke, P., S. Luyssaert, J. Mertens, E. Meers, F. M. Tack, and B. Lust 2003. Phytoremediation prospects of willow stands on contaminated sediment: a field trial. Environ Pollut. 126: 275-282.
97. Vogel, T. M. 1996. Bioaugmentation as a soil bioremediation approach. Curr. Opin. Biotechnol. 7: 311-316.
98. Vogt, C., D. Simon, A. Alfreider, and W. Babel. 2004. Microbial degradation of chlorobenzene under oxygen-limited conditions leads to accumulation of 3-chlorocatechol. Environ. Toxicol. Chem. 23: 265-270.
99. Wild, S. R. and K. C. Jones. 1992. Polynuclear aromatic hydrocarbon uptake by carrot grown in sludge-amended soil. J. Environ. Qual. 21: 217-225.
100. Wiltse, C. C., W. L. Rooney, Z. Chen, A. P. Schwab, and M. K. Banks. 1998. Greenhouse evaluation of agronomic and crude oil-phytoremediation potential among alfalfa genotypes. J. Environ. Qual. 27: 169-173.
101. Wolfe, N. L. and C. F. Hoehamer. 2003. Enzymes used by plants and microorganisms to detoxify organic compounds, p. 159-187. In S. C. McCutcheon and J. L. Schnoor (eds.), Phytoremediation: Transformation and Control of Contaminants, Wiley, New York, U.S.A.
102. 14C-pyrene mineralization. Soil Biol. Biochem. 33: 1769-1776.