pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin

Journal of Neuroscience Methods

Volumn 158, Issue 1, 2006, Pages 56-63

  Chong, Kevin Wai Yin ^a   Lee, Alan Yiu Wah ^a   Koay, Evelyn S C ^a,b   Seet, Sze Jee ^a   Cheung, Nam Sang ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY HOSPITAL   (Singapore)

Author keywords

Mouse; N1E 115; Neuro 2A; Neuroblastoma; Optimised procedure; pH; TransFectin ; Transfection

Indexed keywords

GREEN FLUORESCENT PROTEIN; REAGENT; TRANSFECTIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CANCER CELL CULTURE; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; FLUORESCENCE; GENETIC TRANSFECTION; MOUSE; NERVE CELL; NEUROBLASTOMA; NEUROBLASTOMA CELL; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CATIONS; CELL LINE, TUMOR; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HYDROGEN-ION CONCENTRATION; LIPOSOMES; MICE; NEUROBLASTOMA; TIME FACTORS; TRANSFECTION;

EID: 33749518495     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2006.05.017     Document Type: Article

References (15)

1. Biewenga J.E., Destrbe O.H.J., and Schrama L.H. Plasmid-mediated gene transfer in neurons using the biolistics technique. J Neurosci Methods 71 (1997) 67-75
2. Caplen N.J., Alton E.W., Middleton P.G., Dorin J.R., Stevenson B.J., Gao X., Durham S.R., Jeffery P.K., et al. Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis. Nat Med 1 (1995) 39-46
3. Cheung N.S., Choy M.S., Halliwell B., Teo T.S., Bay B.H., Lee A.Y., Qi R.Z., Koh V.H., et al. Lactacystin-induced apoptosis of cultured mouse cortical neurons is associated with accumulation of PTEN in the detergent-resistant membrane fraction. Cell Mol Life Sci 61 (2004) 1926-1934
4. Dalby B., Cates S., Harris A., Ohki E.C., Tilkins M.L., Price P.J., and Ciccarone V.C. Advanced transfection with Lipofectamine 2000 reagent: primary neurons, siRNA, and high-throughput applications. Methods 33 (2004) 95-103
5. Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M., Northrop J.P., Ringold G.M., and Danielson M. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci USA 84 (1987) 7413-7417
6. Fu H., Hu Y., McNelis T., and Hollinger J.O. A calcium phosphate-based gene delivery system. J Biomed Mater Res A 74 (2005) 40-48
7. Graeser R., Gannon J., Poon R.Y.C., Dubois T., Aitken A., and Hunt T. Regulation of the CDK-related protein kinase PCTAIRE-1 and its possible role in neurite outgrowth in Neuro-2A cells. J Cell Sci 115 (2002) 3479-3490
8. Hawley-Nelson P., Ciccarone V., Gebeyehu G., and Jessee J. LipofectAMINE reagent: a new, higher efficiency polycationic liposome transfection reagent. Focus 15 (1993) 73-79
9. Jacobsen L.B., Calvin S.A., and Colvin K.E. Wright M. FuGENE 6 Transfection Reagent: the gentle power. Methods 33 (2004) 104-112
10. Johnsson M., and Bergstrand N. Phase behavior of DOPE/TritonX100 (reduced) in dilute aqueous solution: aggregate structure and pH-dependence. Colloids Surf B Biointerfaces 34 (2004) 69-76
11. Jordan M., and Wurm F. Transfection of adherent and suspended cells by calcium phosphate. Methods 33 (2004) 136-143
12. Koticha D.K., MaCarthy E.E., and Baldini G. Plasma membrane targeting of SNAP-25 increases its local concentration and is necessary for SNARE complex formation and regulated exocytosis. J Cell Sci 115 (2002) 3341-3351
13. Kranenburg O., Scharnhorst V., Van der Eb A.J., and Zantema A. Inhibition of cyclin-dependent kinase activity triggers neuronal differentiation of mouse neuroblastoma cells. J Cell Biol 131 (1995) 227-234
14. Nakanishi M., and Noguchi A. Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative. Adv Drug Deliver Rev 52 (2001) 197-207
15. Radler J.O., Koltover I., Salditt T., and Safinya C.R. Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science 275 (1997) 810-814