Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products

Molecular and Cellular Probes

Volumn 19, Issue 5, 2005, Pages 299-305

  Cremonesi, Paola ^a   Luzzana, Massimo ^a   Brasca, Milena ^b   Morandi, Stefano ^b   Lodi, Roberta ^b   Vimercati, Chiara ^a   Agnellini, Dario ^a   Caramenti, Giancarlo ^c   Moroni, Paolo ^a   Castiglioni, Bianca ^d,e  

^a UNIVERSITY OF MILAN   (Italy)

^b INSTITUTE OF SCIENCES OF FOOD PRODUCTION   (Italy)

^c INSTITUTE OF BIOMEDICAL TECHNOLOGIES   (Italy)

^d CNR   (Italy)

^e NONE   (Italy)

Author keywords

Dairy products; Enterotoxigenic Staphylococcus aureus; Milk; Multiplex PCR

Indexed keywords

BACTERIAL TOXIN; RNA 23S;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; DAIRY PRODUCT; DIAGNOSTIC ACCURACY; ELECTROIMMUNOASSAY; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA GENE; STAPHYLOCOCCUS AUREUS;

ANIMALS; DAIRY PRODUCTS; ENTEROTOXINS; FOOD CONTAMINATION; HUMANS; MILK; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 23S; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 24644473099     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2005.03.002     Document Type: Article

References (36)

1. M.A. Khan, C.H. Kim, I. Kakoma, E. Morin, R.D. Hansen, and W.L. Hurley Detection of Staphylococcus aureus in milk by use of polymerase chain reaction analysis Am J Vet Res 59 1998 807 813
2. J.R. Middleton, L.K. Fox, J.M. Gay, J.W. Tyler, and T.E. Besser Use of pulsed-field gel electrophoresis for detecting differences in Staphylococcus aureus strain populations between dairy herds with different cattle importation practices Epidemiol Infect 129 2002 387 395
3. M.M. Dinges, P.M. Orwin, and P.M. Schlievert Enterotoxins of Staphylococcus aureus Clin Microbiol Rev 13 2000 16 34
4. N. Balaban, and A. Rasooly Review: staphylococcal enterotoxins Int J Food Microbiol 61 2000 1 10
5. S.R. Monday, and G.A. Bohach Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates J Clin Microbiol 37 1999 3411 3414
6. S. Tamarapu, J.L. McKillip, and M. Drake Development of a multiplex polymerase chain reaction assay for detection and differentiation of Staphylococcus aureus in dairy products J Food Prot 64 2001 664 668
7. S. Jarraud, G. Cozon, F. Vandenesch, M. Bes, J. Etienne, and G. Lina Involvement of enterotoxins G and I in staphylococcal toxic syndrome and staphylococcal scarlet fever J Clin Microbiol 37 1999 2446 2449
8. S.H. Munson, M.T. Tremaine, M.J. Betley, and R.A. Welch Identification and characterisation of staphylococcal enterotoxin types G and I from Staphylococcus aureus Infect Immun 66 1998 3337 3348
9. K. Ren, J.D. Bannan, V. Pancholi, A.L. Cheung, J.C. Robbins, and V.A. Fischietti Characterisation and biological properties of a new staphylococcal exotoxin J Exp Med 180 1994 1675 1683
10. P.M. Orwin, D.Y. Leung, H.L. Donahue, R.P. Novick, and P.M. Schlievert Biochemical and biological properties of staphylococcal enterotoxin K Infect Immun 69 2001 360 366
11. S. Zhang, J.J. Iandolo, and C. Stewart The enterotoxin D plasmid of Staphylococcus aureus encodes a second enterotoxin determinant (sej) FEMS Microbiol Lett 168 1998 227 233
12. R. Stephan, C. Annemuller, A.A. Hassan, and Ch. Lammler Characterisation of enterotoxigenic Staphylococcus aureus strains isolated from bovine mastitis in north-east Switzerland Vet Microbiol 38 2001 373 382
13. C. Letertre, S. Perelle, F. Dilasser, and P. Fach Identification of a new putative enterotoxin SEU encoded by the egc cluster of Staphylococcus aureus J Appl Microbiol 95 2003 38 43
14. K. Omoe, M. Ishikawa, Y. Shimoda, D.L. Hu, S. Ueda, and K. Shinagawa Detection of seg, seh and sei genes in Staphylococcus aureus isolates and determination of enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes J Clin Microbiol 40 2002 857 862
15. N.K. Sharma, C.D. Rees, and C.E.R. Dodd Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains Appl Environ Microbiol 66 2000 1347 1353
16. M.S. Bergdoll Enterotoxins C.S.F. Easton C. Adlam Staphylococci and staphylococcal infections 1983 Academic Press London 559 598
17. G. Najera-Sanchez, R. Maldonado-Rodriguez, P.R. Olvera, and L. Mota de la Garza Development of two multiplex polymerase chain reaction for the detection of enterotoxigenic strains of Staphylococcus aureus isolated from foods J Food Prot 66 2003 1055 1062
18. P. Forsman, A. Tilsala-Timisjarvi, and T. Alatossava Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions Microbiology 143 1997 3491 3500
19. M.A. Godkin, and K.E. Leslie Culture of bulk tank milk as a mastitis screening test: a brief review Can Vet J 34 1993 601 605
20. T-R. Chen, M.H. Hsiao, C.S. Chiou, and H.Y. Tsen Development and use of PCR primers for the investigation of C1, C2 and C3 enterotoxin types of Staphylococcus aureus strains isolated from food-borne outbreaks Int J Food Microbiol 71 2001 63 70
21. O. Akineden, C. Annemuller, A.A. Hassan, C. Lammler, W. Wolter, and M. Zschock Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis Clin Diagn Lab Immunol 8 2001 959 964
22. P. Phuektes, G.F. Browning, G. Anderson, and P. Mansell Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in bulk milk samples J Dairy Res 70 2003 149 155
23. J.P. Rosec, and O. Gigaud Staphylococcal enterotoxin genes of classical and new types detected by PCR in France Int J Food Microbiol 77 2002 61 70
24. R.L. Amann, W. Ludwig, and K.H. Schleifer Phylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol Rev 59 1995 143 169
25. J.A. Straub, C. Hertel, and W.P. Hammes A 23S rDNA-targeted polymerase chain reaction-based system for detection of Staphylococcus aureus in meat starter cultures and dairy products J Food Prot 62 1999 1150 1156
26. C. Su, I. Kanevsky, B.M. Jayarao, and L.M. Sordillo Phylogenetic relationships of Staphylococcus aureus from bovine mastitis based on coagulase gene polymorphism Vet Microbiol 71 2000 53 58
27. J. Schlegelova, M. Dendis, J. Benedik, V. Babak, and D. Rysanek Staphylococcus aureus isolates from dairy cows and humans on a farm differ in coagulase genotype Vet Microbiol 92 2003 327 334
28. P.E. Carter, K. Begbie, and F.M. Thomson-Carter Coagulase gene variants associated with distinct populations of Staphylococcus aureus Epidemiol Infect 130 2003 207 219
29. P. Barski, L. Piechwicz, J. Galinski, and J. Kur Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR Mol Cell Probes 10 1996 471 475
30. P.M. Orwin, J.R. Fitzgerald, D.Y.M. Leung, J.A. Gutierrez, G.A. Bohach, and P.M. Schlievert Characterisation of Staphylococcus aureus enterotoxin L Infect Immun 71 2003 2916 2919
31. R.W. Bennet, and F. McClure Visual screening with enzyme immunoassay for staphylococcal enterotoxins in food: collaborative study J AOAC Int 77 1999 357 364
32. Y. Su, and A.C.L. Wong Current perspective on detection of staphylococcal enterotoxins J Food Prot 60 1997 195 202
33. A.A. Wienke Comparison of four kits for the detection of staphylococcal enterotoxin in foods from outbreaks of food poisoning Int J Food Microbiol 14 1991 305 312
34. O.G. Brakstad, K. Aasbakk, and J.A. Maeland Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene J Clin Microbiol 30 1992 1654 1660
35. K. Becker, A.W. Friedrich, G. Lubritz, M. Weilert, G. Peters, and C. Eiff Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens J Clin Microbiol 41 2003 1434 1439
36. G. Blaiotta, D. Ercolini, C. Pennecchia, V. Fusco, A. Cosaburi, and O. Pepe PCR detection of staphylococcal enterotoxin genes in Staphylococcus spp. Strains isolated from meat and dairy products. Evidence from new variants of SEG and SEI in S. aureus AB-8802 J Appl Microbiol 97 2004 719 730