The reliability of relative anion-cation permeabilities deduced from reversal (dilution) potential measurements in ion channel studies

Cell Biochemistry and Biophysics

Volumn 46, Issue 2, 2006, Pages 143-154

  Barry, Peter H ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Anion cation permeability ratios; Brownian dynamics; Counter ion permeation; Goldman Hodgkin Katz equation; Irreversible thermodynamics; Ligand gated ion channels; Planck equation; Reversal potentials

Indexed keywords

ANION; CATION; ION CHANNEL;

ARTICLE; BIOLOGICAL MODEL; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL MEMBRANE POTENTIAL; CHANNEL GATING; METHODOLOGY; PATCH CLAMP; PHYSIOLOGY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY;

ANIONS; CATIONS; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; ION CHANNEL GATING; ION CHANNELS; MEMBRANE POTENTIALS; MODELS, BIOLOGICAL; PATCH-CLAMP TECHNIQUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 33749250735     PISSN: 10859195     EISSN: None     Source Type: Journal    
DOI: 10.1385/CBB:46:2:143     Document Type: Article

References (26)

1. Goldman, D. E. (1943) Potential, impedance and rectification in membranes. J. Gen. Physiol. 27, 37-60.
2. Hodgkin, A. L., and Katz, B. (1949) The effects of sodium ions on the electrical activity of the giant axon of the squid. J. Physiol. (Lond.) 108, 37-77.
3. Blatz, A. L., and Magleby, K. L. (1985) Single chloride-selective channels active at resting membrane potentials in cultured rat skeletal muscle. Biophys. J. 47, 119-123.
4. Franciolini, F., and Nonner, W. (1987) Anion and cation permeability of a chloride channel in rat hippocampal neurons. J. Gen. Physiol. 90, 453-478.
5. Bormann, J., Hamill, O. P., and Sakmann, B. (1987) Mechanism of anion permeation through channels gated by glycine and γ-aminobutyric acid in mouse cultured spinal neurones. J. Physiol. (Lond.) 385, 243-286.
6. Keramidas, A., Moorhouse, A. J., French, C. R., Schofield, P. R., and Barry, P. H. (2000) M2 pore mutations convert the glycine receptor channel from being anion- to cation-selective. Biophys. J. 78, 247-259.
7. Keramidas, A., Moorhouse, A. J., Pierce, K. D., Schofield, P. R., and Barry, P. H. (2002) Cation-selective mutations of the inhibitory glycine receptor channel reveal determinants of ion-charge selectivity. J. Gen. Physiol. 119, 393-410.
8. 3A receptor from cationic to anionic reveals a conserved feature of the ligand-gated ion channel superfamily. J. Biol. Chem. 276, 10977-10983.
9. 3 receptor. Brit. J. Pharmacol. 140, 359-365.
10. C ρ1 receptors invert ion selectivity and influence open state probability. J. Biol. Chem. 279, 54153-54160.
11. O'Mara, M., Barry, P. H., and Chung, S. H. (2003) A model of the glycine receptor deduced from Brownian dynamics studies. Proc. Natl. Acad. Sci. U. S. A. 100, 4310-4315.
12. Barry, P. H., and O'Mara, M. L. (2005) The reliability of relative cation-anion permeabilities deduced from reversal (dilution) potential measurements in ion channel studies and Brownian dynamics predictions. FASEB J. 19(5) Abstracts, Part III, A1170, Abstract 671.87.
13. Cheng, M. H., Cascio, M., and Coalson R. D. (2005) Theoretical studies of the M2 transmembrane segment of the glycine receptor: models of the open pore structure and current-voltage characteristics. Biophys. J. 89, 1669-1680.
14. Barry, P. H., and Lynch, J. W. (1991) Topical review. Liquid junction potentials and small cell effects in patch clamp analysis. J. Membr. Biol. 121, 101-117.
15. Barry, P. H. (1994) JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial and bilayer measurements and for correcting junction potential measurements. J. Neurosci. Meth. 51, 107-116.
16. Barry, P. H., and Diamond, J. M. (1971) A theory of ion permeation through membranes with fixed neutral sites. J. Membrane Biol. 4, 295-330.
17. Katchalsky, A., and Curran, P. F. Nonequilibrium Thermodynamics in Biophysics. Harvard University Press, Cambridge, MA, 1967.
18. Keramidas, A., Moorhouse, A. J., Schofield, P. R., and Barry, P. H. (2004) Ligand gated ion channels: mechanisms underlying ion selectivity. Prog. Biophys. Mol. Biol. 86, 161-204.
19. MacInnes, D. A. The Principles of Electrochemistry. Dover Publications, New York, 1961
20. Im W., Seefeld, S., and Roux B. (2000) A grand canonical Monte Carlo-Brownian dynamics algorithm for simulating ion channels. Biophys. J. 79, 788-801.
21. Patlak, C. S. (1960) Derivation of an equation for the diffusion potential. Nature 188, 944-945.
22. Sandblom, J. P., and Eisenman, G. (1967) Membrane potential at zero current. The significance of a constant ionic permeability ratio. Biophys. J. 7, 217-242.
23. Barry, P. H., and Gage, P. W. (1984) Ion selectivity of channels at the end-plate. Curr. Top. Membr. Transport 21, 1-51.
24. Hille, B. Ion Channels of Excitable Membranes. 3rd ed. Sinauer Assoc., Inc., Sunderland, MA, 2001.
25. Wu, J. (1991) Microscopic model for selectivity permeation in ion channels. Biophys. J. 60, 238-251.
26. Ussing, H. H. (1949) The distinction by means of tracers between active transport and diffusion. Acta Physiol. Scand. 19, 43-56.