Global cDNA amplification combined with real-time RT-PCR: Accurate quantification of multiple human potassium channel genes at the single cell level

Yeast

Volumn 17, Issue 3, 2000, Pages 201-210

  Ai Taher, A ^a   Bashein, A ^a   Nolan, T ^a   Hollingsworth, M ^a   Brady, G ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Quantitative; Real time PCR; RT PCR; Single cell

Indexed keywords

EID: 33749208030     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Alberts B, Bray D, Lewis J, et al. 1994. Molecular Biology of the Cell. 3rd ed, New York, Garland.
2. Anwer K, Oberti C, Perez GJ, et al. 1993. Calcium-activated K + channels as modulators of human myometrial contractile activity. Am J Physiol 265: C976-985.
3. Anwer K, Toro L, Oberti C, Stefani E, Sanborn BM. 1992. Ca(2 + )-activated K+ channels in pregnant rat myometrium: modulation by a -adrenergic agent. Am J Physiol 263: C1049-1056.
4. Art JJ, Wu YC, Fettiplace R. 1995. The calcium-activated potassium channels of turtle hair-cells. J Gen Physiol 105: 49-72.
5. Berardi AC, Wang A, Levine JD, Lopez P, Scadden DT. 1995. Functional isolation and characterization of human hematopoietic stem cells. Science 267: 104-108.
6. Brady G, Barbara M, Iscove NN. 1990. Representative in vitro cDNA amplification from individual hemopoietic cells and colonies. Methods Mol Cell Biol 2: 17-25.
7. Brady G, Billia F, Knox J, et al. 1995. Analysis of gene-expression in a complex differentiation hierarchy by global amplification of cDNA from single cells. Curr Biol 5: 909-922.
8. Brady G, Iscove NN. 1993. Construction of cDNA libraries from single cells. Methods Enzymol 225: 611-623.
9. Brail LH, Jang A, Billia F, el al. 1999. Gene expression in individual cells: analysis using global single cell reverse transcription polymerase chain reaction (GSC RT-PCR). Mutai Res 406: 45-54.
10. Cheng T, Shen H, Giokas D, et al. 1996. Temporal mapping of gene expression levels during the differentiation of individual primary hematopoietic cells. Proc Natl Acad Sci USA 93: 13158-13163.
11. Cumano A, Paige CJ, Iscove NN, Brady G. 1992. Bipotential precursors of B cells and macrophages in murine fetal liver. Nature 356: 612-615.
12. Duggan DJ, Bittner M, Chen YD, Meltzer P, Trent JM. 1999. Expression profiling using cDNA microarrays. Nature Genet 21: 10-14.
13. Dulac C, Axel R. 1995. A novel family of genes encoding putative pheromone receptors in mammals. Cell S3: 195-206.
14. Häuser NC, Vingron M, Scheideier M, el al. 1998. Transcriptional profiling on all open reading frames of Saccharomyces cererisiae. Yeast 14: 1209-1221.
15. Ishii TM, Silvia C, Hirschberg B, et al. 1997. A human intermediate conductance calcium-activated potassium channel. Proc Nail Acad Sci USA 94: 11651-11656.
16. Kaczorowski GJ, Knaus HG, Leonard RJ, McManus OB, Garcia ML. 1996. High-conductance calcium-activated potassium channels; structure, pharmacology and function. J Bioenergelics Biomcmbrancs 28: 255-267.
17. Khan RN, Smith SK, Morrison JJ, Ashford ML. 1993. Properties of large-conductance K+ channels in human myometrium during pregnancy and labour. Proc R Soc Loud B Biol Sci 251: 9-15.
18. Khan RN, Smith SK, Morrison JJAshfordML. 1997. Ca2 + dependcncc and pharmacology of large-conductance K + channels in nonlabor and labor human uterine myocytes. Am J Physiol 213: C1721-1731.
19. Logsdon NJ, Kang JS, Togo JA, Christian EP, Aiyar J. 1997. A novel gene hKCa4 encodes the calcium-activated potassium channel in human T lymphocytes. J Biol Client 272: 32723-32726.
20. Nelson MT, Cheng H, Rubart M, Santana LF, Bonev AD, Knot HJ, Lederer WJ. 1995. Relaxation of arterial smooth muscle by calcium sparks. Science 270: 633-637.
21. Norman MR, Thompson EB. 1977. Characterization of a glucocorticoid-sensitive human lymphoid cell line. Cancer Res 37: 3785-3791.
22. Núnêz C, Bashein AM, Brunei C, el al. 2000. Expression of the imprinted tumour-suppressor gene H19 is tightly regulated during normal hematopoiesis and is reduced in hematopoietic precursors of patients with polycythemia vera. J Pathol 190: 61-68.
23. Prcudhomme C, Revillion F, Merlat A, et al. 1999. Detection of BCR-ABL transcripts in chronic myeloid leukemia (CML) using a 'real time' quantitative RT-PCR assay. Leukemia 13: 957-964.
24. Ramanathan K, Michael TH, Jiang GJ, Hiel H, Fuchs PA. 1999. A molecular mechanism for electrical tuning of cochlear hair cells. Science 283: 215-217.
25. Rambhatla L, Patel B, Dhanasekaran N, Latham KE. 1995. Analysis of G protein alpha subunit mRNA abundance in preimplantation mouse embryos using a rapid quantitative RT-PCR approach. Mol Reproil Dev 41: 314-324.
26. Rappolee DA, Wang A, Mark D, Werb Z. 1989. Novel method for studying messenger-RNA phenotypes in single or small numbers of cells. J Cell Biochem 39: 1-11.
27. Sauvageau G, Lansdorp PM, Eaves CJ, el al. 1994. Differential expression of homeobox genes in functionally distinct CD34( + ) subpopulations of human bone-marrow cells. Proc Natl Acad Sci USA 91: 12223-12227.
28. Song M, Zhu N, Olcese R, et al. 1999. Hormonal control of protein expression and mRNA levels of the MaxiK channel alpha subunit in myometrium. FEBS Lett 460: 427-432.
29. Steuerwald N, Cohen J, Herrera RJ, Brenner CA. 1999. Analysis of gene expression in single oocytes and embryos by real-time rapid cycle fluorescence monitored RT-PCR. Mol Hum ReprodS: 1034-1039.
30. Trumper LH, Brady G, Bagg A, et al. 1993. Single-cell analysis of Hodgkin and Reed-Sternberg cells: molecular heterogeneity of gene expression and p53 mutations. Blood 81: 3097-3115.
31. Tseng-Crank J, Godinot N, Johansen TE, et al. 1996. Cloning expression and distribution of a Ca2 +-activated K+ channel bcta-subunit from human brain. Proc Natl Acad Sci USA 93: 9200-9205.
32. Wallner M, Meera P, Ottolia M, et al. 1995. Characterization of and modulation by a /?-subunit of a human maxi KCa channel cloned from myometrium. Receptors Channels 3: 185-199.
33. Wang T, Brown MJ. 1999. mRNA quantification by real time TaqMan polymerase chain reaction: validation and comparison with RNase protection. Anal Biochem 269: 198-201.
34. Van XQ, Brady G, Iscove NN. 1993. Platelet-derived growth factor (PDGF) activates primitive hematopoietic precursors (pre-CFCmulti) by upregulating IL-1 in PDGF receptorexpressing macrophages. J Imiminol 150: 2440-2448.