Detection of BCR-ABL transcripts in chronic myeloid leukemia (CML) using a 'real time' quantitative RT-PCR assay

Leukemia

Volumn 13, Issue 6, 1999, Pages 957-964

  Preudhomme, C ^a,b   Revillion F ^c   Merlat, A ^a,b   Hornez, L ^c   Roumier, C ^a,b   Duflos Grardel, N ^a,b   Jouet, J P ^a   Cosson, A ^a,b   Peyrat, J P ^c   Fenaux, P ^a,b  

^a LILLE UNIVERSITY HOSPITAL   (France)

^b INSERM   (France)

^c CENTRE OSCAR LAMBRET   (France)

Author keywords

Bone marrow transplantation; Chronic myeloid leukemia; Minimal residual disease; Quantitative RT PCR

Indexed keywords

DNA POLYMERASE; EXONUCLEASE; FLUORESCENT DYE; RECOMBINANT ALPHA INTERFERON; RECOMBINANT INTERFERON;

ADULT; ALLOGENIC BONE MARROW TRANSPLANTATION; ARTICLE; AUTOLOGOUS BONE MARROW TRANSPLANTATION; CALIBRATION; CHROMOSOME 9; CHROMOSOME TRANSLOCATION; CHRONIC MYELOID LEUKEMIA; CLINICAL ARTICLE; CYTOGENETICS; ENZYME ACTIVITY; EXON; FEMALE; GENE AMPLIFICATION; GENE SEQUENCE; GRANULOCYTIC SARCOMA; HUMAN; HUMAN CELL; MALE; MINIMAL RESIDUAL DISEASE; PRIORITY JOURNAL; PROTO ONCOGENE; RELAPSE; REMISSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNIQUE;

EID: 0344142489     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.leu.2401426     Document Type: Article

References (20)

1. Heisterkamp N, Stam K, Groffen J, de Klein A, Grosveld G. Structural organization of the bcr gene and its role in the Ph1 translocation. Nature 1985; 315: 758-761.
2. Kurzrock R, Gutterman J, Talpaz M. The molecular genetics of Philadelphia-chromosome-positive leukemias. New Engl J Med 1988; 319: 990-998.
3. Roth MS, Antin JH, Bingham EL, Ginsburg D. Detection of Philadelphia chromosome positive cells by the polymerase chain reaction following bone marrow transplant for chronic myelogenous leukemia. Blood 1989; 74: 882-885.
4. Delfau MH, Kerckaert JP, Collyn d'Hoodghe M, Fenaux P, Lai JL, Jouet JP, Grandchamp B. Detection of minimal residual disease in chronic myeloid leukemia patients after bone marrow transplantation by polymerase chain reaction. Leukemia 1990; 4: 1-5.
5. Preudhomme C, Wattel E, Lai JL, Henic N, Meyer L, Noel MP, Cosson A, Jouet JP, Fenaux P. Good predictive value of combined cytogenetic and molecular follow-up in chronic myelogenous leukemia after non-T cell-depleted allogeneic bone marrow transplantation: a report on 38 consecutive cases. Leuk Lymphoma 1995; 18: 265-271.
6. Lion T, Izraeli S, Henn T, Gaiger A, Mor W, Gadner H. Monitoring of residual disease in chronic myelogenous leukemia by quantitative polymerase chain reaction. Leukemia 1992; 6: 495-499.
7. Hughes TP, Morgan GJ, Martial P, Goldman JM. Detection of residual leukemia after bone marrow transplant for chronic myeloid leukemia: role of polymerase chain reaction in predicting relapse. Blood 1991; 77: 874-878.
8. Gaiger A, Lion T, Kalhs P, Mitterbauer G, Henn T, Haas O, Fodinger M, Kier P, Forstinger C, Quehenberger P, Hinterberger W, Jager UW, Linkesch W, Mannhalter C, Lechner K. Frequent detection of BCR-ABL specific mRNA in patients with chronic myeloid leukemia (CML) following allogeneic and syngeneic bone marrow transplantation (BMT). Leukemia 1993; 7: 1766-1772.
9. Guerrasio A, Martinelli C, Saglio G, Rosso C, Zaccaria C, Rosti G, Testoni N, Abrosetti A, Izzi T, Sessarego M, Frassoni F, Gasparini P, Chiamenti A, di Bartolomeo P, Pignatti PF. Minimal residual disease status in transplanted chronic myelogenous leukemia patients: low incidence of polymerase chain reaction positive cases among 48 long disease-free subjects who received unmanipulated allogeneic bone marrow transplants. Leukemia 1992; 6: 507-512.
10. Pignon JM, Henri T, Amselem S, Vidaud M, Duquesnoy P, Vernant JP, Kuentz M, Cordonnier C, Rochant H, Goossen M. Frequent detection of minimal residual disease by use of the polymerase chain reaction in long-term survivors after bone marrow transplantation for chronic myeloid leukemia. Leukemia 1990; 4: 83-86.
11. Thompson JD, Brodsky I, Yunis JJ. Molecular quantification of residual disease in chronic myelogenous leukemia after bone marrow transplantation. Blood 1992; 79: 1629-1635.
12. Cross NCP, Feng L, Chase A, Bungey J, Hughes TP, Goldman JM. Competitive polymerase chain reaction to estimate the number of BCR-ABL transcripts in chronic myeloid leukemia patients after bone marrow transplantation. Blood 1993; 82: 1929-1936.
13. Malingue MC, Mahon FX, Delfau MH, Daheron L, Kitzis A, Guilhot F, Tanzer J, Grandchamp B. Quantitative determination of the hybrid Bcr-Abl RNA in patients with chronic myelogenous leukemia under interferon therapy. Br J Haematol 1992; 82: 701-707.
14. Hochhaus A, Lin F, Reiter A, Skladny H, Mason P, van Rhee F, Sherpherd CA, Allan NC, Hehlmann R, Goldman JM, Cross NCP. Quantification of residual disease in chronic myelogenous leukemia patients on interferon-α therapy by competitive polymerase chain reaction. Blood 1996; 87: 1549-1555.
15. Heid CA, Stevens J, Livak KJ, Williams PM. Real-time quantitative PCR. Genome Res 1996; 6: 986-994.
16. Livak KJ, Flood SJA, Marmaro J, Gusti W, Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Meth Appl 1995; 4: 357-362.
17. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991; 88: 7276-7280.
18. Mensink E, van de Locht A, Schattenberg A, Linders E, Schaap N, Geurts van Kessel A, de Witte T. Quantification of minimal residual disease in Philadelphia chromosome positive chronic myeloid leukaemia patients using real-time quantitative RT-PCR. Br J Haematol 1998; 102: 768-774.
19. Marcucci G, Livak KJ, Bi W, Strout MP, Bloomfield CD, Caligiuri MA. Detection of minimal residual disease in patients with AML1/ETO-associated acute myeloid leukemia using a novel quantitative reverse transcription polymerase chain reaction assay. Leukemia 1998; 12: 1482-1489.
20. Kolb H-J, Schattenberg A, Goldman JM, Hertenstein B, Jacobsen N, Arcese W. Graft-versus-leukemia effect of donor lymphocyte transfusions in marrow grafted patients. Blood 1995; 86: 2041-2050.