Application of Real Time PCR in post transplant monitoring of cytomegalovirus infection: Comparison with other diagnostic approaches

New Microbiologica

Volumn 29, Issue 3, 2006, Pages 185-192

  Gaeta, Aurelia ^a,b   Nazzari, Cristina ^a   Verzaro, Simona ^a   Latte, Maria Cristina ^a   Fabri, Giovanna ^a   Mancini, Carlo ^a  

^a Clinical Microbiology   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Cytomegalovirus; Real Time PCR; Transplant

Indexed keywords

ARTICLE; COMPARATIVE STUDY; CYTOMEGALIC INCLUSION BODY DISEASE; CYTOMEGALOVIRUS; ENZYME IMMUNOASSAY; EVALUATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; ORGAN TRANSPLANTATION; POLYMERASE CHAIN REACTION; POSTOPERATIVE COMPLICATION;

CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; HUMANS; IMMUNOENZYME TECHNIQUES; ORGAN TRANSPLANTATION; POLYMERASE CHAIN REACTION; POSTOPERATIVE COMPLICATIONS;

CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5;

EID: 33749182056     PISSN: 11217138     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. AQUINO, V.H., FIGUEIREDO, L.T., BRAZ, J. (2001). Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR. Med. Biol. Res. 34 (1): 93-101.
2. BARBER, L., EGAN, M.D., LOMAX, J., HAIDER, Y., YONAN, N., WOODCOOK, A.A., TURNER, A.J., Fox, A.J. (2000). A prospective study of a Quantitative PCR Elisa assay for the diagnosis of CMV pneumonia in lung and heart-transplant recipients. J. Heart Lung Transplant. (19): 771-780.
3. BOECKH, M., BOIVIN, G. (1998). Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clinical Microbiology Reviews 11: 533-554.
4. BONON, S.H., MENONI, S.M., ROSSI, C.L., DE SOUZA, C.A., VIGORITO, A.C., COSTA, D.B., COSTA, S.C. (2005). Surveillance of cytomegalovirus infection in haematopoietic stem cell transplantation patients. J. Infect. 50 (2) 130-7.
5. CUNNINGHAM, R., HARRIS, A., FRANKTON, A., IRVING, W. (1995). Detection of cytomegalovirus using PCR in serum from renal transplant recipients. J. Clin. Pathol. (48) 575-577.
6. CALIENDO, A.M., ST GEORGE, K., KAO, S.Y., ALLEGA, J., TAN, B.H., LAFONTAINE, R., BUI, L., RINALDO, C.R. (2000). Comparison of quantitative cytomegalovirus (CMV) PCR in plasma and CMV antigenemia assay: clinical utility of the prototype AMPLICOR CMV MONITOR test in transplant recipients. J. Clin. Microbiol. (38) 2122-2127.
7. DELGADO, R., LUMBRERAS, C., ALBA, C., PEDRAZA, M.A., OTERO, J.R., GOMEZ, R., MORENO, E., NORIEGA, A.R, PAYA, C.V. (1992). Low predictive value of polymerase chain reaction for diagnosis of CMV disease in liver transplant recipients. J. Clin. Microbiol. 30: 1876-1878.
8. FAN, J., MA, W.H., QIAN, J., CHEN, Z., HUANG, D.S., WANG, W.L., ZHENG, S.S. (2002). Cytomegalovirus infection in liver transplant recipients. Hepatobiliary Pancreat Dis Int. (1) 30-32.
9. FOX, J.C., KID, I.M., GRIFFITHS, P.D., SWENY, P., EMERY, V.C. (1995). Longitudinal analysis of Cytomegalovirus load in renal transplant recipients using a quantitative polymerase chain reaction: correlation with disease. J. Gen. Virol. 76: 303-319.
10. GAULT, E., MICHEL, Y., DEHEE, A., BELABANI, C., NICOLAS, J.C., GARBARG-CHENON, A. (2001). Quantification of human cytomegalovirus DNA by real-time PCR. J. Clin. Microbiol. 39: 772-775.
11. GERNA, G., BALDANTI, F., REVELLO, M.G. (2004). Pathogenesis of Human Cytomegalovirus infection and cellular targets. Hum. Immunol. 65: 381-386.
12. GERNA, G., FURIONE, M., BALDANTI, F., SARASINI, A. (1994). Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J. Clin. Microbiol. 32: 2709-2717.
13. GERNA, G., ZAVATTONI, M., BALDANTI, F., SARACINI, A., CHEZZI, L., GROSSI, P., REVELLO, M.G. (1998). Human Cytomegalovirus leukodnaemia correlates more closely with clinical symptoms that antigenemia and viremia in heart and heart-lung transplant recipients with primary HCMV infection. Transplantation 65: 1378-1385.
14. GERNA, G., ZIPETO, D., PAREA, M., REVELLO, M.G., SILINI, E., PERCIVALLE, E., ZAVATTONI, M., GROSSI, P., MILANESI, G. (1991). Monitoring of human Cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia and DNAemia. J. Infect. Dis. 164: 488-498.
15. HADAYA, K., WUNDERLI, W., DEFFERNEZ, C., MARTIN, P.Y., MENTHA, G., BINET, I., PERRIN, L., KAISER, L. (2003): Monitoring of cytomegalovirus infection in solid-organ transplant recipients by an ultrasensitive plasma PCR assay. J Clin Microbiol. 41(8): 3757-3764.
16. HASSAN-WALKER, A.F., KIDD, I.M., SABIN, C., SWENY, P., GRIFFITHS, P.D., EMERY VC. (1999). Quantity of human cytomegalovirus (CMV) DNAemia as a risk factor for CMV disease in renal allograft recipients: relationship with donor/recipient CMV serostatus, receipt of augmented methylprednisolone and antithymocyte globulin (ATG). J. Med. Virol. (58): 182-187.
17. HEID, C.A., STEVENS, J., LIVAK, K.J. AND WILLIAMS, P.M. (1996): Real Time Quantitative PCR. Genome research 6: 986-994.
18. HO, M. (1991): Acquired Cytomegalovirus infection in immunocompetent patients. In Plenum Medical Book Company: "Cytomegalovirus biology and infection." New York. 229-247.
19. HO, M. (1991). Human Cytomegalovirus infections in immunosuppressed patients. In Plenum Medical Book Company: "Cytomegalovirus biology and infection." New York. 249-300.
20. HUMAR, A., GREGSON, D., CALIENDO, A.M., MCGEER, A., MALKAN, G., KRAJDEN, M., COREY, P., GREIG, P., WALMSLEY, S., LEVY, G., MAZZULLI, T. (1999). Clinical utility of quantitative cytomegalovirus virsal load determination for predicting cytomegalovirus disease in liver transplant recipients. Transplantatio 68: 1305-1311.
21. KWOK, S. (1990): Procedures to minimize PCR-product carry-over. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols. A guide to methods and applications. San Diego, CA: Academic Press. 142-145.
22. LANDRY, M.L. AND FERGUSON, D. (1993). Comparison of quantitative Cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J. Clin. Microb. 31: 2851-2856.
23. LIVAK, K.J., FLOOD, S.J., MARMARO, J., GIUSTI, W., DEETZ, K. (1995). Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 4: 357-362.
24. MANEZ, R., KUSNE, S., RINALDO, C., AGUADO, J.M., ST GEORGE, K., GROSSI, P., FRYE, B., FUNG, J.J., EHRLICH, G.D. (1996). Time to detection of cytomegalovirus (CMV) DNA in blood leukocytes is a predictor for the development of CMV disease in CMV-seronegative recipients of allografts from CMV-seropositive donors following liver transplantation. J Infect Dis. 173:1072-1076.
25. MARTIN-DAVILA, P., FORTUN, J., GUTIERREZ, C., MARTI-BELDA, P., CANDELAS, A., HONRUBIA, A., BARCENA, R., MARTINEZ, A., PUENTE, A., DE VICENTE, E., MORENO, S. J. (2005). Analysis of a quantitative PCR assay for CMV infection in liver transplant recipients: an intent to find the optimal cut-off value. Clin Virol. 33 (2): 138-44.
26. NAZZARI, C., GAETA, A., LAZZARINI, M., DELLI CASTELLI, T. AND MANCINI, C. (2000). Multiplex Polymerase Chain Reaction for the evaluation of Cytomegalovirus DNA load in organ transplant recipients. Journal of Medical Virology 61: 251-258.
27. NAJIOULLAH, F., THOUVENOT, D., LINA, B. (2001. Development of a real-time PCR procedure including an internal control for the measurement of HCMV viral load. J. Virol. Methods 92: 55-64.
28. NUMATA, S., NAKAMURA, Y., IMAMURA, Y., HONDA, J., MOMOSAKI, S., KOJIRO, M. (2005). Rapid quantitative analysis of human cytomegalovirus DNA by the real-time polymerase chain reaction method. Arch Pathol Lab Med. 129 (2): 200-204.
29. PATEL, R., SMITH, T.F., ESPY, M., PORTELA, D., WIESNER, R.H., KROM, R.A., PAYA, C.V. (1995). A prospective comparison of molecular diagnostic techniques for the early detection of Cytomegalovirus in liver transplant recipients. J. Infct. Dis. 171: 1010-1014.
30. PIIPARINEN, H., HELANTERA, I., LAPPALAINEN, M., SUNI, J., KOSKINEN, P., GRONHAGEN-RISKA, C., LAUTENSCHLAGER, I. (2005). Quantitative PCR in the diagnosis of CMV infection and in the monitoring of viral load during the antiviral treatment in renal transplant patients. J Med Virol. 76 (3) 367-372.
31. ROLLAG, H., SAGEDAL, S., KRISTIANSEN, K.I., KVALE, D., HOLTER, E., DEGRE, M., NORDAL, K.P. (2002). Cytomegalovirus DNA concentration in plasma predicts development of cytomegalovirus disease in kidney transplant recipients. Clin Microbiol Infect. 8 (7): 431-434.
32. SANCHEZ, J.L., KRUGER, R.M., PARANJOTHI, S., TRULOCK, E.P., LYNCH, J.P., HICKS, C., SHANNON, W.D., STORCH, G.A. (2001). Relationship of cytomegalovirus viral load in blood to pneumonitis in lung transplant recipients (72): 733-735.
33. SANCHEZ, J.L., STORCH, G.A. (2002). Multiplex, quantitative, real-time PCR assay for cytomegalovirus and human DNA. J Clin Microbiol. 40 (7): 2381-2386.
34. SALTZMAN, R.L., QUIRK, M.R., JORDAN, M.C. (1992.: High levels of circulating cytomegalovirus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients. J. Clin. Invest. 90: 1832-1838.
35. SCHAFER, P., LAUFS, R. (1996). Experience with quantitative PCR for the management of HCMV disease. Intervirolgy. 39: 204-212.
36. SCHVOERER, E., HENRIOT, S., ZACHARY, P., FREITAG, R., FUCHS, A., FRITSCH, S., RISCH, S., MEYER, N., CAILLARD, S., LIOURE, B., STOLL-KELLER, F. (2005). Monitoring low cytomegalovirus viremia in transplanted patients by a real-time PCR on plasma. J Med Virol. 76(1): 76-81.
37. SIA, I.G. AND PATEL, R. (2000). New Strategies for Prevention and Therapy of Cytomegalovirus Infection and Disease in Solid-Organ Transplant Recipients. Clin Microbiol Rev. 13 (1) 83-121.
38. TARRAGO, D., MATEOS, M.L., AVELLON, A., PEREZ-VAZQUEZ, M.D., TENORIO, A. (2004). Quantitation of cytomegalovirus DNA in cerebrospinal fluid and serum specimens from AIDS patients using a novel highly sensitive nested competitive PCR and the cobas amplicor CMV monitor. J Med Virol. 72 (2): 249-256.
39. ZIPETO, D., REVELLO, M.G., SILINI, E., PAREA, M., PERCIVALLE, E., ZAVATTONI, M., MILANESI, G., GERNA, G. (1992). Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human Cytomegalovirus DNA in blood samples from immunocompromised patients. J. Clin. Microb. 30: 527-530.