Comparison of quantitative cytomegalovirus (CMV) PCR in plasma and CMV antigenemia assay: Clinical utility of the prototype AMPLICOR CMV MONITOR test in transplant recipients

Journal of Clinical Microbiology

Volumn 38, Issue 6, 2000, Pages 2122-2127

  Caliendo, Angela M ^a,b,f   St George, Kirsten ^c   Kao, Shaw Yi ^d   Allega, Jessica ^a   Tan, Ban Hock ^a,g   LaFontaine, Robert ^d   Bui, Larry ^d   Rinaldo, Charles R ^c,e  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^d ROCHE MOLECULAR SYSTEMS   (United States)

^e UNIVERSITY OF PITTSBURGH   (United States)

^f EMORY UNIVERSITY HOSPITAL   (United States)

^g SINGAPORE GENERAL HOSPITAL   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; VIRUS DNA;

ADULT; ARTICLE; ASSAY; CHILD; CYTOMEGALOVIRUS; DIAGNOSTIC VALUE; FEMALE; HUMAN; INTERMETHOD COMPARISON; MALE; PLASMA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TRANSPLANTATION; VIRUS DETECTION;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; DNA VIRUSES;

EID: 0034046010     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.6.2122-2127.2000     Document Type: Article

References (45)

1. Abecassis, M. M., A. J. Koffron, B. Kaplan, M. Buckingham, J. P. Muldoon, A. J. Cribbins, D. B. Kaufman, J. P. Fryer, J. Stuart, and F. P. Stuart. 1997. The role of PCR in the diagnosis and management of CMV in solid organ transplant recipients. Transplantation 63:275-279.
2. Aitken, C., W. Barrett-Muir, C. Millar, K. Templeton, J. Thomas, F. Sheridan, D. Jefferies, M. Yaqoob, and J. Breuer. 1999. Use of molecular assays in diagnosis and monitoring of cytomegalovirus disease following renal transplantation. J. Clin. Microbiol. 37:2804-2807.
3. Boeckh, M., G. M. Gallez-Hawkins, D. Myerson, J. Zaia, and R. A. Bowden. 1997. Plasma polymerase chain reaction for cytomegalovirus DNA after allogeneic bone marrow transplantation. Transplantation 64:108-113.
4. Brennan, D. C., K. A. Garlock, B. A. Lippmann, R. S. Buller, M. Gaudreault-Keener, J. A. Lowell, S. B. Miller, S. Shenoy, T. K. Howard, and G. A. Storch. 1997. Contrai of cytomegalovirus-associated morbidity in renal transplant recipients using intensive monitoring and either preemptive or deferred therapy. J. Am. Soc. Nephrol. 8:118-125.
5. Cassol, S. A., M. C. Poon, R. Pal, M. J. Naylor, J. Culver-James, T. J. Bowen, J. A. Russell, S. A. Krawetz, R. T. Pon, and D. I. Hoar. 1989. Primer-mediated enzymatic amplification of CMV DNA. J. Clin. Investig. 83:1109-1115.
6. Collins, L. A., M. H. Samore, M. S. Roberts, R. Luzzati, R. L. Jenkins, W. D. Lewis, and A. W. Karchmer. 1994. Risk factors for invasive fungal infection complicating orthotopic liver transplantation. J. Infect. Dis. 170:644-652.
7. Cope, A. V., C. Sabin, A. Burroughs, K. Rolles, P. D. Griffiths, and V. C. Emery. 1997. Interrelationships among quantity of human cytomegalovirus (HCMV) DNA in blood, donor-recipient setostatus, and administration of methylprednisolone as risk factors for HCMV disease following liver transplantation. J. Infect. Dis. 176:1484-1490.
8. de Otero, J., J. Gavalda, E. Murio, V. Vargas, I. Calico, L. Llopart, J. Rossello, C. Margarit, and A. Pahissa. 1998. Cytomegalovirus disease as a risk factor for graft loss and death after orthotopic liver transplantation. Clin. Infect. Dis. 26:865-870.
9. Einsele, H., G. Ehninger, H. Hebart, K. M. Wittkowski, U. Schuler, G. Jahn, P. Mackes, M. Herter, T. Klingebiel, J. Loffler, S. Wagner, and C. A. Muller. 1995. Polymerase chain reaction monitoring reduces the incidence of cytomegalovirus disease and the duration and side effects of antiviral therapy after bone marrow transplantation. Blood 86:2815-2820.
10. Falagas, M. E., D. R. Snydman, J. Griffith, B. G. Werner, and the Boston Center for Liver Transplantation CMVIG Study Group. 1996. Exposure to cytomegalovirus from the donated organ is a risk factor for bacteremia in orthotopic liver transplant recipients. Clin. Infect. Dis. 23:468-474.
11. Fishman, J. A., and R. H. Rubin. 1998. Infection in organ-transplant recipients. N. Engl. J. Med. 338:1741-1751.
12. Gerna, G., D. Zipeto, M. Parea, M. G. Revello, E. Silini, E. Percivalle, M. Zavattoni, P. Grossi, and G. Milanesi. 1991. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia and DNAemia. J. Infect. Dis. 164: 488-498.
13. Goodrich, J. M., M. More, C. A. Gleaves, C. DuMond, M. Cays, D. F. Ebeling, W. C. Buhles, B. DeArmand, and J. D. Meyer. 1991. Early treatment with ganciclovir to prevent cytomegalovirus disease after allogeneic bone marrow transplantation. N. Engl. J. Med. 325:1601-1607.
14. Hebart, H., C. Muller, J. Loffler, G. Jahn, and H. Einsele. 1996. Monitoring of CMV infection: a comparison from whole blood, plasma PCR, pp65-antigenemia and virus culture in patients after bone marrow transplantation. Bone Marrow Transplant. 17:861-868.
15. Hibberd, P. L., N. E. Tolkoff-Rubin, D. Conti, F. Stuart, J. R. Thistlethwaite, J. F. Neylan, D. R. Suydman, R. Freeman, M. I. Lorber, and R. H. Rubin. 1995. Preemptive ganciclovir therapy to prevent cytomegalovirus disease in cytomegalovirus-antibody-positive renal transplant recipients. Ann. Intern. Med. 123:18-26.
16. Humar, A., D. Gregson, A. M. Caliendo, A. McGeer, G. Malkan, M. Krajden, P. Corey, P. Greig, S. Walmsley, G. Levy, and T. Mazzulli. 1999. Clinical utility of quantitative cytomegalovirus viral load determination for predicting cytomegalovirus disease in liver transplant recipients. Transplantation 68: 1305-1311.
17. Jiwa, N. M., G. W. van Gemert, A. K. Raap, F. M. van de Rijke, A. Mulder, P. F. Lens, M. M. M. Salimans, F. E. Zwann, W. van Dorp, and M. van der Ploeg. 1989. Rapid detection of human cytomegalovirus DNA in peripheral blood leucocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation 48:72-76.
18. Kidd, I. M., J. C. Fox, D. Pillay, H. Charman, P. D. Griffiths, and V. C. Emery. 1993. Provision of prognostic information in immunocompromised patients by routine application of the polymerase chain reaction for cytomegalovirus. Transplantation 56:867-871.
19. Koehler, M., K. St. George, G. D. Ehrlich, J. Mirro, S. M. Neudorf, and C. Rinaldo. 1997. Prevention of CMV disease in allogeneic BMT recipients by CMV antigenemia-guided preemptive therapy. J. Pediatr. Hematol. Oncol. 19:43-47.
20. Koskinen, P. T., S. N. Markku, S. P. Mattila, P. J. Hayry, and I. T. Lautenschlager. 1993. The correlation between symptomatic CMV infection and CMV antigenemia in heart allograft recipients. Transplantation 55:547-551.
21. Kouzarides, T., A. T. Banjier, S. C. Satchwell, K. Weston, P. Tomlinson, and B. G. Barell. 1987. Sequence and transcription analysis of the human cytomegalovirus DNA polymerase gene. J. Virol. 61:125-133.
22. Landry, M. L., and D. Ferguson. 1993. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J. Clin. Microbiol. 31:2851-2856.
23. Mazzulli, T., R. H. Rubin, M. J. Ferraro, R. T. D'Aquila, S. A. Doveikis, B. R. Smith, T. H. The, and M. S. Hirsch. 1993. Cytomegalovirus antigenemia: clinical correlations in transplant recipients and in persons with AIDS. J. Clin. Microbiol. 31:2824-2827.
24. Nolle, F. S., R. K. Emmens, C. Thurmond, P. S. Mitchell, C. Pascuzzi, S. M. Devine, R. Saral, and J. R. Wingard. 1995. Early detection of human cytomegalovirus viremia in bone marrow transplant recipients with DNA amplification. J. Clin. Microbiol. 33:1263-1266.
25. Pasternack, R., P. Vuorinen, T. Pitkajarvi, M. Koskela, and A. Miettinen. 1997. Comparison of manual Amplicor PCR, Cohas Amplicor PCR, and Lex assays for detection of Chlamydia trachomatis infection in women by using urine specimens. J. Clin. Microbiol. 35:402-405.
26. Patel, R., T. F. Smith, M. Espy, D. Portela, R. H. Wiesner, R. A. F. Krom, and C. V. Paya. 1995. A prospective comparison of molecular diagnostic techniques for the early detection of cytomegalovirus in liver transplant recipients. J. Infect. Dis. 171:1010-1014.
27. Reinke, P., E. Fietze, S. Ode-Hakim, S. Prosch, J. Lippert, R. Ewert, and H.-D. Volk. 1994. Late-acute renal allograft rejection and symptomless cytomegalovirus infection. Lancet 344:1737-1738.
28. Roberts, T. C., D. C. Brennan, R. S. Buller, M. Gaudreault-Keener, M. A. Schnitzler, K. E. Sternhell, K. A. Garlock, G. G. Singer, and G. A. Storch. 1998. Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients. J. Infect. Dis. 178:626-635.
29. Rubin, R. H. 1991. Preemptive therapy in immunocompromised hosts. N. Engl. J. Med. 324:1057-1059.
30. Rubin, R. H. 1994. Infection in the organ transplant recipient. Plenum Medical Book Co., New York, N.Y.
31. Rubin, R. H. 1998. Cytomegalovirus disease and allograft loss after organ transplantation. Clin. Infect. Dis. 26:871-873.
32. Rubin, R. H., and N. E. Tolkoff-Rubin. 1993. Antimicrobial strategies in the care of organ transplant recipients. Antimicrob. Agents Chemother. 37:619-624.
33. Spector, S. A., K. Hsia, D. Wolf, M. Shinkai, and I. Smith. 1995. Molecular detection of human cytomegalovirus and determination of genotypic ganciclovir resistance in clinical specimens. Clin. Infect. Dis. 21(Suppl. 2):S170-S173.
34. St. George, K., and C. R. Rinaldo. 1997. Comparison of commercially available antibody reagents for the cytomegalovirus pp65 antigenemia assay. Clin. Diagn. Virol. 7:147-152.
35. Storch, G. A., R. S. Buller, T. C. Bailey, N. A. Ettinger, T. Langlois, M. Gaudreault-Keener, and P. L. Welby. 1994. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. J. Clin. Microbiol. 32:997-1003.
36. Tanabe, K., T. Tokumoto, N. Ishikawa, I. Koyama, K. Takahashi, S. Fuchinoue, T. Kawai, S. Koga, T. Yagisawa, H. Toma, K. Ota, and H. Nakajima. 1997. Comparative study of CMV antigenemia assay, polymerase chain reaction, serology, and shell vial assay in the early diagnosis and monitoring of CMV infection after renal transplantation. Transplantation 64:1721-1725.
37. Tong, C. Y., L. Cuevas, H. Williams, and A. Bakran. 1998. Use of laboratory assays to predict cytomegalovirus disease in renal transplant recipients. J. Clin. Microbiol. 36:2681-2685.
38. van den Berg, A. P., I. J. Klompmaker, E. B. Haagsma, P. M. Peeters, L. Meerman, R. Verwer, T. H. The, and M. J. Slooff. 1996. Evidence for an increased rate of bacterial infections in liver transplant recipients with cytomegalovirus infection. Clin. Transplant. 10:224-231.
39. van den Berg, A. P., L. Meyaard, S. A. Otto, W. J. van Son, I. J. Klompmaker, G. Mesander, L. H. F. M. de Leij, F. Miedema, and T. H. The. 1995. Cytomegalovirus infection associated with decreased proliferative capacity and increased rate of apoptosis of peripheral blood lymphocytes. Transplant. Proc. 27:936-938.
40. van der Bij, W., J. Schirm, R. Torensma, W. J. van Son, A. M. Tegzess, and T. H. The. 1988. Comparison between viremia and antigenemia for the detection of cytomegalovirus in blood. J. Clin. Microbiol. 26:2531-2535.
41. van der Bij, W., R. Torensma, W. J. van Son, J. Anema, J. Schirm, A. M. Tegzess, and T. H. The. 1988. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes. J. Med. Virol. 25:179-188.
42. van Dorp, W. T., A. Vlieger, N. M. Jiwa, L. A. van Es, M. van der Ploeg, J. L. C. M. van Saase, and F. J. van der Woude. 1992. The polymerase chain reaction, a sensitive and rapid technique for detecting cytomegalovirus infection after renal transplantation. Transplantation 54:661-664.
43. Wirgart, B. Z., K. Claesson, B. M. Eriksson, M. Brundin, G. Tufveson, T. Totterman, and L. Grillner. 1996. Cytomegalovirus (CMV) DNA amplification from plasma compared with CMV pp65 antigen (ppUL83) detection in leukocytes for early diagnosis of symptomatic CMV infection in kidney transplant recipients. Clin. Diagn. Virol. 7:99-110.
44. Wolf, D. G., and S. A. Spector. 1993. Early diagnosis of human cytomegalovirus disease in transplant recipients by DNA amplification in plasma. Transplantation 56:330-334.
45. Wolff, C., M. Skourtopoulos, D. Hornschemeyer, D. Wolff, M. Korner, F. Hufert, R. Korfer, and K. Kleesiek. 1996. Significance of human cytomegalovirus DNA detection in immunocompromised heart transplant patients. Transplantation 61:750-757.