High-throughput assays for DNA gyrase and other topoisomerases

Nucleic Acids Research

Volumn 34, Issue 15, 2006, Pages

  Maxwell, Anthony ^a   Burton, Nicolas P ^a   O'Hagan, Natasha ^a  

^a JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DNA TOPOISOMERASE; DNA TOPOISOMERASE (ATP HYDROLYSING); DNA TOPOISOMERASE IV; FLUORESCENT DYE; OLIGONUCLEOTIDE; PLASMID DNA;

ARTICLE; CONTROLLED STUDY; DNA CONTENT; DNA DETERMINATION; DNA SEQUENCE; DNA SUPERCOILING; ESCHERICHIA COLI; EUKARYOTE; FLUORESCENCE ANGIOGRAPHY; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; ISOTOPE LABELING; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; RESTRICTION MAPPING; ENZYME ASSAY; ENZYMOLOGY; GENETICS; METABOLISM; METHODOLOGY; PLASMID;

ESCHERICHIA COLI; EUKARYOTA;

DNA GYRASE; DNA TOPOISOMERASE IV; DNA, BACTERIAL; ENZYME TESTS; ESCHERICHIA COLI; PLASMIDS;

EID: 33749134234     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkl504     Document Type: Article

References (17)

1. Champoux,J.J. (2001) DNA topoisomerases: Structure, function, and mechanism. Annu. Rev. Biochem., 70, 369-413.
2. Corbett,K.D. and Berger,J.M. (2004) Structure, molecular mechanisms, and evolutionary relationships in DNA topoisomerases. Annu. Rev. Biophys. Biomol. Struct., 33, 95-118.
3. Maxwell,A. (1997) DNA gyrase as a drug target. Trends Microbiol., 5, 102-109.
4. Hande,K.R. (1998) Clinical applications of anticancer drugs targeted to topoisomerase II. Biochim. Biophys. Acta, 1400, 173-184.
5. Wall,M.K., Mitchenall,L.A. and Maxwell,A. (2004) Arabidopsis thaliana DNA gyrase is targeted to chloroplasts and mitochandria. Proc. Natl. Acad. Sci. USA, 101, 7821-7826.
6. Sun,J.S., Garestier,T. and Helene,C. (1996) Oligonucleotide directed triple helix formation. Curr. Opin. Struct. Biol., 6, 327-333.
7. Gilbert,D.E. and Feigon,J. (1999) Multistranded DNA structures. Curr. Opin. Struct. Biol., 9, 305-314.
8. Gowers,D.M. and Fox,K.R. (1999) Towards mixed sequence recognition by triple helix formation. Nucleic Acids Res., 27, 1569-1577.
9. Fox,K.R. (2000) Targeting DNA with triplexes. Curr. Med. Chem., 7, 17-37.
10. Firman,K. and Szczelkun,M.D. (2000) Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement. EMBO J., 19, 2094-2102.
11. McClelland,S.E., Dryden,D.T. and Szczelkun,M.D. (2005) Continuous assays for DNA translocation using fluorescent triplex dissociation: application to type I restriction endonucleases. J, Mol. Biol., 348, 895-915.
12. Simon,H., Fortsch,I., Burckhardt,G., Gabrielyan,A., Birch-Hirschfeld,E., Stelzner,A., Prevot-Halter,I., Leumann,C. and Zimmer,C. (1999) Triple helix formation inhibits DNA gyrase activity. Antisense Nucleic Acid Drug Dev., 9, 527-531.
13. Hanvey,J.C., Shimizu,M. and Wells,R.D. (1988) Intramolecular DNA triplexes in supercoiled plasmids. Proc. Natl. Acad. Sci. USA, 85, 6292-6296.
14. Sakamoto,N., Akasaka,K., Yamamoto,T. and Shimada,H. (1996) A triplex DNA structure of the polypyrimidine:pOlypurine stretch in the 5′ flanking region of the sea urchin arylsulfatase gene. Zoolog. Sci., 13, 105-109.
15. Kawabata,Y., Ooya,T., Lee,W.K. and Yui,N. (2002) Self-assembled plasmid DNA network prepared through both triple-helix formation and streptavidin-biotin interaction. Macromol. Biosci., 2, 195-198.
16. Boros,I., Pósfai,G. and Venetianer,P. (1984) High-copy-number derivatives of the plasmid cloning vector pBR322. Gene, 30, 257-260.
17. Reece,R.J. and Maxwell,A. (1989) Tryptic fragments of the Escherichia coli DNA gyrase A protein. J. Biol. Chem., 264, 19648-19653.