Molecular cloning, stable expression and cellular localization of human α1-adrenergic receptor subtypes: Effect of charcoal/dextran treated serum on expression and localization of α1D-adrenergic receptor

Biotechnology Letters

Volumn 28, Issue 21, 2006, Pages 1731-1739

  Khattar, Sunil K ^a   Bora, Roop Singh ^a   Priyadarsiny, Priyanka ^a   Gautam, Aarti ^a   Gupta, Dikshi ^a   Tiwari, Atul ^a   Nanda, Kamna ^a   Singh, Rahul ^a   Chugh, Anita ^a   Bansal, Vinay ^a   Mookhtiar, Kasim ^a   Saini, Kulvinder Singh ^a  

^a RANBAXY RESEARCH LABORATORIES   (India)

Author keywords

Adrenergic receptors; Cellular localization; Charcoal dextran treated fetal bovine serum; Stable cell lines

Indexed keywords

ADRENERGIC RECEPTORS; CELLULAR LOCALIZATION; CHARCOAL/DEXTRAN TREATED FETAL BOVINE SERUM; IMMUNOFLUORESCENCE; STABLE CELL LINES;

BODY FLUIDS; CELL MEMBRANES; DNA; IMMUNOLOGY; MOLECULAR STRUCTURE; POLYSACCHARIDES;

GENETIC ENGINEERING;

ADRA1A PROTEIN, HUMAN; ADRA1B PROTEIN, HUMAN; ADRA1D PROTEIN, HUMAN; ADRENERGIC RECEPTOR ALPHA(1A); ADRENERGIC RECEPTOR ALPHA(1B); ADRENERGIC RECEPTOR ALPHA(1D); ALPHA 1 ADRENERGIC RECEPTOR; CHARCOAL; COMPLEMENTARY DNA; DEXTRAN; RECOMBINANT PROTEIN;

ANIMAL EMBRYO; ARTICLE; CELL CULTURE; CELL LINE; CELL MEMBRANE; CHEMISTRY; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; EMBRYO; FETUS BLOOD; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; KIDNEY; METABOLISM; MOLECULAR CLONING; SERUM;

CELL CULTURE TECHNIQUES; CELL LINE; CELL MEMBRANE; CELLS, CULTURED; CHARCOAL; CLONING, MOLECULAR; CULTURE MEDIA; DEXTRANS; DNA, COMPLEMENTARY; EMBRYO; EMBRYO, MAMMALIAN; FETAL BLOOD; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HUMANS; KIDNEY; RECEPTORS, ADRENERGIC, ALPHA-1; RECOMBINANT PROTEINS; SERUM;

BOS TAURUS;

EID: 33749016454     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9148-x     Document Type: Article

References (23)

1. Cotecchia S, Schwinn DA, Randall RR, Lefkowitz RJ, Caron MG, Kobilka BK (1988) Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor. Proc Natl Acad Sci USA 85:7159-7163
2. Garcia-Sainz JA, Torres-Padilla ME (1999) Modulation of basal intracellular calcium by inverse agonists and phorbol myristate acetate in rat-1 fibroblasts stably expressing a1D adrenoceptors. FEBS Lett 443:277-281
3. Hague C, Uberti MA, Chen Z, Hall RA, Minneman KP (2004a) Cell surface expression of alpha1D-adrenergic receptors is controlled by heterodimerization with alpha1B-adrenergic receptors. J Biol Chem 279:15541-15549
4. 1D-adrenergic receptor prevents cell surface expression. J Pharmacol Exp Ther 309:388-397
5. Hirasawa A, Sugawara T, Awaji T, Tsumaya K, Ito H, Tsujimoto G (1997) Subtype-specific differences in subcellular localization of alpha1- adrenoceptors: chlorethylclonidine preferentially alkylates the accessible cell surface alpha1-adrenoceptors irrespective of the subtype. Mol Pharmacol 52:52764-52770
6. 1-adrenoceptor subtypes. FEBS Lett 386:141-148
7. Hrometz SL, Edelmann SE, McCune DF, Olges JR, Hadley RW, Perez DM, Piascik MT (1999) Expression of multiple alpha1-adrenoceptors on vascular smooth muscle: correlation with the regulation of contraction. J Pharmacol Exp Ther 290:452-463
8. Khattar SK, Bora RS, Priyadarsiny P, Gupta D, Khanna A, Narayanan KL, Babu V, Chugh A, Saini KS (2006) High level stable expression of pharmacologically active human m1-m5 muscarinic receptor subtypes in Mammalian cells. Biotechnol Lett 28:121-129
9. Lindquist DL, de Alarcon PA (1987) Charcoal-dextran treatment of fetal bovine serum removes an inhibitor of human CFU-megakaryocytes. Exp Hematol 15:234-238
10. Lomasney JW, Cotecchia S, Lorenz W, Leung WY, Schwinn DA, Yang-Feng TL, Brownstein M, Lefkowitz RJ, Caron MG (1991) Molecular cloning and expression of the cDNA for the alpha 1A-adrenergic receptor. The gene for which is located on human chromosome 5. J Biol Chem 266:6365-6369
11. McCune DF, Edelmann SE, Olges JR, Post GR, Waldrop BA, Waugh DJ, Perez DM, Piascik MT (2000) Regulation of the cellular localization and signaling properties of the alpha(1B)- and alpha(1D)-adrenoceptors by agonists and inverse agonists. Mol Pharmacol 57:659-666
12. Perez DM, Piascik MT, Graham RM (1991) Solution-phase library screening for the identification of rare clones: isolation of an alpha 1D-adrenergic receptor cDNA. Mol Pharmacol 40:876-883
13. Perez DM, Piascik MT, Malik N, Gaivin R, Graham RM (1994) Cloning, expression and tissue distribution of the rat homolog of the bovine alpha 1C-adrenergic receptor provide evidence for its classification as the alpha 1A subtype. Mol Pharmacol 46:823-831
14. Piascik MT, Perez DM (2001) Alpha1-adrenergic receptors: new insights and directions. J Pharmacol Exp Ther 298:403-410
15. 1D-adrenoceptors increases expression of binding sites but not protein. Eur J Pharmacol 462:1-8
16. 1B-adrenergic receptor: potential role of protonation and hydrophobicity of a highly conserved aspartate. Proc Natl Acad Sci USA 94:808-813
17. Schwinn DA, Lomasney JW, Lorenz W, Szklut PJ, Fremeau RT Jr, Yang-Feng TL, Caron MG, Lefkowitz RJ, Cotecchia S (1990) Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. J Biol Chem 265:8183-8189
18. 1-adrenoceptor subtypes can affect the subtype selectivity of drugs in a study with the fluorescent ligand BODIPY FL-prazosin. Life Sci. 70:2113-2124
19. Terzic A, Puceat M, Vassort G, Vogel SM (1993) Cardiac alpha 1-adrenoceptors: an overview. Pharmacol Rev 45:147-175
20. Tsang LL, Chan LN, Liu CQ, Chan HC (2001) Effect of phenol red and steroid hormones on cystic fibrosis transmembrane conductance regulator in mouse endometrial epithelial cells. Cell Biol Int 25:1021-1024
21. Uberti MA, Hall RA, Minneman KP (2003) Subtype-specific dimerization of alpha 1 adrenoceptors: effects on receptor expression and pharmacological properties. Mol Pharmacol 64:1379-1390
22. Vicentic A, Robeva A, Rogge G, Uberti M, Minneman KP (2002) Biochemistry and pharmacology of epitope-tagged alpha-1-adrenergic receptor subtypes. J Pharmacol Exp Ther 302:58-65
23. Zhong H, Minneman KP (1999) Alpha1-adrenoceptor subtypes. Eur J Pharmacol 375:261-276