Probing conserved amino acids in phospholipase D (Brassica oleracea var. capitata) for their importance in hydrolysis and transphosphatidylation activity

Protein Engineering, Design and Selection

Volumn 19, Issue 10, 2006, Pages 443-452

  Lerchner, Alexandra ^a   Mansfeld, Johanna ^a   Kuppe, Konstantin ^a   Ulbrich Hofmann, Renate ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Brassica oleracea var. capitata; E.coli; Mutagenesis; Phospholipase D; Transphosphatidylation

Indexed keywords

BRASSICA OLERACEA VAR. CAPITATA; ENZYME VARIANTS; PHOSPHOLIPASE D; TRANSPHOSPHATIDYLATION;

ASSAYS; ENZYME KINETICS; ESCHERICHIA COLI; GLYCEROL; HYDROLYSIS; HYDROPHOBICITY; MUTAGENESIS; PHOSPHOLIPIDS; SUBSTITUTION REACTIONS;

AMINO ACIDS;

AMINO ACID DERIVATIVE; CYSTEINE; ENZYME VARIANT; GLYCEROL; GLYCEROPHOSPHOLIPID; ISOENZYME; PHOSPHOLIPASE D; PHOSPHOLIPASE D2;

ARTICLE; CABBAGE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; GENE SEQUENCE; GENETIC CONSERVATION; HYDROLYSIS; HYDROPHOBICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT LEAF; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; VARIETAS;

AMINO ACID SEQUENCE; BRASSICA; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; ESCHERICHIA COLI; HYDROLYSIS; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; PHOSPHOLIPASE D; PLANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

BRASSICA OLERACEA VAR. CAPITATA; ESCHERICHIA COLI;

EID: 33748776808     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzl028     Document Type: Article

References (34)

1. Abousalham, A., Riviere, M., Teissere, M. and Verger, R. (1993) Biochim. Biophys. Acta, 1158, 1-7.
2. Brinkmann, U., Mattes, R.E. and Buckel, P. (1989) Gene, 85, 109-114.
3. Carrea, G.D., D'Arrigo, P., Piergianni, V., Roncaglio, S., Secundo, F. and Servi, S. (1995) Biochim. Biophys. Acta, 1255, 273-279.
4. D'Arrigo, P., Piergianni, V., Scarcelli, D. and Servi, S. (1995) Anal. Chim. Acta, 304, 249-254.
5. Heller, M. (1978) Adv. Lipid Res., 16, 267-326.
6. Hirche, F., Koch, M.H.J., König, S., Wadewitz, T. and Ulbrich-Hofmann, R. (1997) Enzyme Microb Technol., 20, 453-461.
7. Hirche, F. and Ulbrich-Hofmann, R. (2000) Biocatal. Biotransformation, 18, 343-353.
8. Hwang, I.-S., Park, S.-J., Roh, T.-Y., Choi, M.-U. and Kim, H.J. (2001) Rapid Commun. Mass Spectrom., 15, 110-115.
9. Iwasaki, Y., Nishiyama, T., Kawaraski, Y., Nakano, H. and Yamane, T. (2000) J. Biosci. Bioeng., 89, 506-508.
10. Juneja, L.R., Kazuoka, T., Yamane, T. and Shimizu, S. (1988) Biochim. Biophys. Acta, 960, 334-341.
11. Kyte, J. and Doolittle, R.F. (1982) J. Mol. Biol., 157, 105-132.
12. Laemmli, U.K. (1970) Nature, 227, 680-685.
13. Lambrecht, R. and Ulbrich-Hofmann, R. (1992) Biol. Chem. Hoppe-Seyler, 373, 81-88.
14. Leiros, I., Secundo, F., Zambonelli, C., Servi, S. and Hough, E. (2000) Structure, 8, 655-667.
15. Leiros, I., McSweeney, S. and Hough, E. (2004) J. Mol. Biol., 339, 805-820.
16. Lerchner, A., Mansfeld, J., Schäffner, I., Schöps, R., Beer, H.K. and Ulbrich-Hofmann, R. (2005) Biochim. Biophys. Acta, 1737, 94-101.
17. Liu, M.Y., Gutowski, S. and Sternweis, P.C. (2001) J. Biol. Chem., 276, 5556-5562.
18. Nalefski, E.A. and Falke, J.J. (1996) Protein Sci., 5, 2375-2390.
19. Novotna, Z., Kas, J., Daussant, J., Sajdok, J. and Valentova, O. (1999) Plant Physiol. Biochem., 37, 531-537.
20. Oblozinsky, M., Schöps, R. , Ulbrich-Hofmann, R. and Bezakova, L. (2003) Biochim. Biophys. Acta, 1631, 153-159.
21. Oblozinsky, M., Ulbrich-Hofmann, R. and Bezakova, L. (2005) Biotechnol. Lett., 27, 181-185.
22. Pannenberg, I., Mansfeld, J. and Ulbrich-Hofmann, R. (1998) Plant Physiol., 118, 1102.
23. Ponting, C.P. and Kerr, I.D. (1996) Protein Sci., 5, 914-922.
24. Qin, C., and Wang, X. (2002) Plant Physiol., 128, 1057-1068.
25. Rost, B. (1996) Methods Enzymol., 266, 525-539.
26. Schäffner, I., Rücknagel, K.-P., Mansfeld, J. and Ulbrich-Hofmann, R. (2002) Eur. J. Lipid Sci. Technol., 104, 79-87.
27. Schöps, R., Schierhorn, A., Schäffner, I., Mansfeld, J. and Ulbrich-Hofmann, R. (2002) J. Protein Chem., 21, 407-411.
28. Servi, S. (1999) Topics Curr. Chem., 200, 127-158.
29. Sung, T.C., Roper, R.L., Zhang, Y., Temel, R., Hammond, S.M., Morris, A.J., Moss, B., Engebrecht, J. and Frohman, M.A. (1997) EMBO J., 16, 4519-4530.
30. Touchstone, J.C., Levin, S.S., Dobbins, M.F. and Beers, P.C. (1983) J. Liq. Chromatogr., 6, 179-192.
31. Ulbrich-Hofmann, R. (2000) In Bornscheuer, U.T. (eds), Enzymes in Lipid Modification, Wiley-VCH, Weinheim, pp 219-262.
32. Ulbrich-Hofmann, R., Lerchner, A., Oblozinsky, M. and Bezakova, L. (2005) Biotechnol. Lett., 27, 535-543.
33. Wang, X. (2000) Prog. Lipid Res., 39, 109-149.
34. Xie, Z., Ho, W.T. and Exton, J.H. (2000) Eur. J. Biochem., 267, 7138-7146.