Characterization of β-hexosaminidase secretion in rabbit lacrimal gland

Experimental Eye Research

Volumn 83, Issue 5, 2006, Pages 1081-1088

  Andersson, Sofia V ^a   Edman, Maria C ^a   Bekmezian, Arpi ^b   Holmberg, Jens ^a,c   Mircheff, Austin K ^b   Peter Gierow, J ^a,b  

^a LINNAEUS UNIVERSITY   (Sweden)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c Pharmexa AS   (Denmark)

Author keywords

cell culture; enzyme; lacrimal gland; secretion; signal transduction; tears

Indexed keywords

BETA N ACETYLHEXOSAMINIDASE; CARBACHOL; CATHEPSIN B; CHELERYTHRINE; PHORBOL ESTER; PILOCARPINE; PROTEIN KINASE C; PROTEIN KINASE C ACTIVATOR; PROTEIN KINASE C INHIBITOR;

ACINAR CELL; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL ISOLATION; ENZYME ACTIVITY; ENZYME RELEASE; IN VIVO STUDY; LACRIMAL FLUID; LACRIMAL GLAND; LACRIMATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; RABBIT;

ALKALOIDS; ANIMALS; BENZOPHENANTHRIDINES; BETA-N-ACETYLHEXOSAMINIDASE; BETA-N-ACETYLHEXOSAMINIDASES; CARBACHOL; CATHEPSIN B; CELLS, CULTURED; CHOLINERGIC AGONISTS; CULTURE MEDIA, SERUM-FREE; ENZYME INHIBITORS; FEMALE; LACRIMAL APPARATUS; LYSOSOMES; PHORBOL ESTERS; PROTEIN KINASE C; RABBITS; SIGNAL TRANSDUCTION; TEARS;

EID: 33748704929     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2006.05.013     Document Type: Article

References (35)

1. Andersson S.V., Sjögren E.C., Magnusson C., and Gierow J.P. Sequencing, expression and enzymatic characterization of β-hexosaminidase in rabbit lacrimal gland and primary cultured acinar cells. Glycobiology 15 (2005) 211-220
2. Azzarolo A.M., Brew K., Kota S., Ponomareva O., Schwartz J., and Zylberberg C. Presence of tear lipocalin and other major proteins in lacrimal fluid of rabbits. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 138 (2004) 111-117
3. Barrett A.J., and Heath M.F. Lysosomal enzymes. In: Dingle J.T. (Ed). Lysosomes, a Laboratory Handbook (1977), Elsevier, Amsterdam 118-120
4. Barrett A.J., and Kirschke H. Cathepsin B, cathepsin H, and cathepsin L. Methods Enzymol. 80 (1981) 535-561
5. Botelho S.Y., and Martinez E.V. Electrolytes in lacrimal gland fluid and in tears at various flow rates in the rabbit. Am. J. Physiol. 225 (1973) 606-609
6. Bradley M.E., Lambert R.W., Lambert R.W., Lee L.M., and Mircheff A.K. Isolation and subcellular fractionation analysis of acini from rabbit lacrimal glands. Invest. Ophthalmol. Vis. Sci. 33 (1992) 2951-2965
7. Bromberg B.B., and Welch M.H. Lacrimal protein secretion: comparison of young and old rats. Exp. Eye Res. 40 (1985) 313-320
8. Cripps M.M., Bromberg B.B., Patchen-Moor K., and Welch M.H. Adrenocorticotropic hormone stimulation of lacrimal peroxidase secretion. Exp. Eye Res. 45 (1987) 673-683
9. Da Costa S.R., Yarber F.A., Zhang L., Sonee M., and Hamm-Alvarez S.F. Microtubules facilitate the stimulated secretion of beta-hexosaminidase in lacrimal acinar cells. J. Cell Sci. 111 (1998) 1267-1276
10. Dartt D.A., Baker A.K., Vaillant C., and Rose P.E. Vasoactive intestinal peptide stimulation of protein secretion from rat lacrimal gland acini. Am. J. Physiol 247 (1984) G502-G509
11. Gachon A.-M.F., Kpamegan G., Kantelip B., and Dastugue B. Relationship between lacrimal gland, isolated cells (lacrimocytes) and tears: biochemical and histological studies in the rabbit eye. Curr. Eye Res. 9 (1986) 647-654
12. Gierow J.P., Lambert R.W., and Mircheff A.K. Fluid phase endocytosis by isolated rabbit lacrimal gland acinar cells. Exp. Eye Res. 60 (1995) 511-525
13. Gierow J.P., Yang T., Bekmezian A., Liu N., Norian J.M., Kim S.A., Rafisolyman S., Zeng H., Okamoto C.T., Wood R.L., and Mircheff A.K. NaK-ATPase in lacrimal gland acinar cell endosomal system: correcting a case of mistaken identity. Am. J. Physiol. 271 (1996) c1685-c1698
14. Gierow J.P., and Mircheff A.K. Role of protein kinases in regulation of apical secretion and basal-lateral membrane recycling traffic in reconstituted rabbit lacrimal gland acini. Adv. Exp. Med. Biol. 438 (1998) 187-190
15. Gilbard J.P., and Dartt D.A. Changes in rabbit lacrimal gland fluid osmolarity with flow rate. Invest. Ophthalmol. Vis. Sci. 23 (1982) 804-806
16. Hamm-Alvarez S.F., da Costa S., Yang T., Wei X., Gierow J.P., and Mircheff A.K. Cholinergic stimulation of lacrimal acinar cells promotes redistribution of membrane-associated kinesin and the secretory protein, β-hexosaminidase, and increases kinesin motor activity. Exp. Eye Res. 64 (1997) 141-156
17. Herzog V., and Miller F. The localization of endogenous peroxidase in the lacrimal gland of the rat during postnatal development. J. Cell Biol. 53 (1972) 662-680
18. Hodges R.R., Raddassi I., Zoukhri D., Toker A., Kazlauskas A., and Dartt D.A. Effect of overexpression of constitutively active PKCalpha on rat lacrimal gland protein secretion. Invest. Ophthalmol. Vis. Sci. 45 (2004) 3974-3981
19. Lambert R.W., Maves C.A., Gierow J.P., Wood R.L., and Mircheff A.K. Plasma membrane internalization and recycling in rabbit lacrimal acinar cells. Invest. Ophthalmol. Vis. Sci. 33 (1993) 305-316
20. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
21. Mahuran D.J. Beta-hexosaminidase: biosynthesis and processing of the normal enzyme, and identification of mutations causing Jewish Tay-Sachs disease. Clin. Biochem 28 (1995) 101-106
22. Mahuran D.J. Biochemical consequences of mutations causing the GM2 gangliosidoses. Biochim. Biophys. Acta 1455 (1999) 105-138
23. Meneray M.A., Fields T.Y., Bromberg B.B., and Moses R.L. Morphology and physiologic responsiveness of cultured rabbit lacrimal acini. Invest. Ophthalmol. Vis. Sci. 35 (1994) 4144-4158
24. Putney J.W., Van de Walle C.M., and Leslie B.A. Stimulus-secretion coupling in the rat lacrimal gland. Am. J. Physiol. 235 (1978) C188-C198
25. Qian L., Wang Y., Xie J., Rose C.M., Yang T., Nakamura T., Sandberg M., Zeng H., Schechter J.E., Chow R.H., Hamm-Alvarez S.F., and Mircheff A.K. Biochemical changes contributing to functional quiescence in lacrimal gland acinar cells after chronic ex vivo exposure to a muscarinic agonist. Scand. J. Immunol. 58 (2003) 550-565
26. Rismondo V., Gierow J.P., Lambert R.W., Golchini K., Feldon S.E., and Mircheff A.K. Rabbit lacrimal acinar cells in primary culture. Morphology and acute responses to cholinergic stimulation. Invest. Ophthalmol. Vis. Sci. 35 (1994) 1176-1183
27. Rose C.M., Qian L., Hakim L., Wang Y., Jerdeva G.Y., Marchelletta R., Nakamura T., Hamm-Alvarez S.F., and Mircheff A.K. Accumulation of catalytically active proteases in lacrimal gland acinar cell endosomes during chronic ex vivo muscarinic receptor stimulation. Scand. J. Immunol. 61 (2005) 36-50
28. Schechter J., Stevenson D., Chang D., Chang N., Pidgeon M., Nakamura T., Okamoto C.T., Trousdale M.D., and Mircheff A.K. Growth of purified lacrimal acinar cells in matrigel raft cultures. Exp. Eye Res. 74 (2002) 349-360
29. Sjögren E.C., Carrington S.D., Corfield A.P., Hicks S.J., and Gierow J.P. Regulated secretion of acid glycosidases by rabbit lacrimal acinar cells in primary culture. Invest. Ophthalmol. Vis. Sci 41 (2000) s62
30. Ubels J.L., Rismondo V., and Osgood T.B. The relationship between secretion of retinol and protein by the lacrimal gland. Invest. Ophthalmol. Vis. Sci. 30 (1989) 952-960
31. Van Haeringen N.J., and Glasius E. The origin of some enzymes in tear fluid, determined by comparative investigation with two collection methods. Exp. Eye Res. 22 (1976) 267-272
32. Van Haeringen N.J., and Glasius E. Lysosomal hydrolases in tears and the lacrimal gland: effect of acetylsalicylic acid on the release from the lacrimal gland. Invest. Ophthalmol. Vis. Sci. 19 (1980) 826-829
33. Yang T., Zeng H., Zhang J., Okamoto C.T., Warren D.W., Wood R.L., Bachmann M., and Mircheff A.K. MHC class II molecules, cathepsins, and La/SSb proteins in lacrimal acinar cell endomembranes. Am. J. Physiol. 277 (1999) C994-C1007
34. Yang T., Zeng H., Zhang J., Okamoto C.T., Warren D.W., Wood R.L., Bachmann M., and Mircheff A.K. Stimulation with carbachol alters endomembrane distribution and plasma membrane expression of intracellular proteins in lacrimal acinar cells. Exp. Eye Res. 69 (1999) 651-661
35. Zoukhri D., Hodges R.R., Sergheraert C., Toker A., and Dartt D.A. Lacrimal gland PKC isoforms are differentially involved in agonist-induced protein secretion. Am. J. Physiol. 272 (1997) C263-C269