Detection of localized caspase activity in early apoptotic cells by laser scanning cytometry

Cytometry

Volumn 47, Issue 2, 2002, Pages 81-88

  Telford, William G ^a   Komoriya, Akira ^b   Packard, Beverly Z ^b  

^a NATIONAL CANCER INSTITUTE   (United States)

^b OncoImmunin Inc ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE; DNA;

ANIMAL; APOPTOSIS; DNA FRAGMENTATION; ENZYMOLOGY; FLOW CYTOMETRY; IMAGE CYTOMETRY; METABOLISM; MOUSE; OSTEOSARCOMA; PATHOLOGY; PHYSIOLOGY; PROCEDURES; RAT; THYMOMA; TUMOR CELL CULTURE;

ANIMALS; APOPTOSIS; CASPASES; DNA FRAGMENTATION; DNA, NEOPLASM; FLOW CYTOMETRY; IMAGE CYTOMETRY; MICE; OSTEOSARCOMA; RATS; THYMOMA; TUMOR CELLS, CULTURED;

EID: 33748701019     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.10052     Document Type: Article

References (27)

1. 1 Telford WG, King LE, Fraker PJ. Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. J Immunol 1994 ; 172: 1–16.
2. 2 Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F. Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 1997 ; 27: 1–20.
3. 3 Del Bino G, Darzynkiewicz Z, Degraef C, Mosselmans R, Galand P. Comparison of methods based on annexin V binding, DNA content or TUNEL for evaluating cell death in HL‐60 and adherent MCF‐7 cells. Cell Prolif 1999 ; 32: 25–37.
4. 4 Vermes I, Haanen C, Reutelingsperger C. Flow cytometry of apoptotic cell death. J Immunol Methods 2000 ; 243: 167–190.
5. 5 Henkart PA. ICE family proteases: mediators of all cell death? Immunity 1996 ; 4: 195–201.
6. 6 Ormerod MG, Sun X‐M, Snowden RT, Davies R, Fearhead H, Cohen GM. Increased membrane permeability in apoptotic thymocytes: a flow cytometric study. Cytometry 1993 ; 14: 595–602.
7. 7 Castedo M, Hirsch T, Susin SA, Zamzami N, Marchetti P, Macho A, Kroemer G. Sequential acquisition of mitochondrial and plasma membrane alterations during early lymphocyte apoptosis. J Immunol 1996 ; 157: 512–521.
8. 8 Green DR, Reed JC. Mitochondria and apoptosis. Science 1998 ; 281: 1309–1312.
9. 9 Overbeek R, Yildirim M, Reutelingsperger C, Haanen C. Early features of apoptosis detected by four different flow cytometry assays. Apoptosis 1998 ; 3: 115–120.
10. 10 Earnshaw WC, Martins LM, Kaufmann SH. Mammalian caspases: structure, activation, substrates and functions during apoptosis. Annu Rev Biochem 1999 ; 68: 383–424.
11. 11 Koester SK, Bolton WE. Cytometry of caspases. Methods Cell Biol 2001 ; 63: 487–504.
12. 12 Gorman AM, Hirt UA, Zhivotovsky B, Orrenius S, Ceccatelliu S. Application of a fluorimetric assay to detect caspase activity in thymus tissue undergoing apoptosis in vivo. J Immunol Methods 1999 ; 226: 43–48.
13. 13 Belloc F, Belaund‐Rotureau MA, Lavignolle V, Bascans E, Braz‐Pereira E, Durrieu F, Lacombe F. Flow cytometry of caspase‐3 activation in preapoptotic leukemic cells. Cytometry 2000 ; 40: 151–160.
14. 14 Bedner E, Smolewshi P, Amstad P, Darzynkiewicz Z. Activation of caspases measured in situ by binding of fluorochrome‐labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 2000 ; 260: 308–313.
15. 15 Komoriya A, Packard BZ, Brown MJ, Wu ML, Henkart PA. Assessment of caspase activities in intact apoptotic thymocytes using cell‐permable fluorogenic caspase substrates. J Exp Med 2000 ; 191: 1819–1828.
16. 16 Lazebnik Y, Kaufmann SH, Desnoyers S, Poirier GG, Earnshaw WC. Cleavage of poly(ADP‐ribose) polymerase by proteinase with properties like ICE. Nature 1994 ; 71: 346–347.
17. 17 Packard BZ, Topygin DD, Komoriya A, Brand L. Profluorescent protease substrates: intramolecular dimers described by the exciton model. Proc Natl Acad Sci USA 1996 ; 93: 11640–11645.
18. 18 Packard BZ, Komoriya A, Brotz TM, Henkart PA. Caspase activity in membrane blebs after anti‐Fas ligation. J Immunol 2001 ; 167: 5061–5066.
19. 19 Zhivotovsky B, Samali A, Gahm A, Orrenius S. Caspases: their intracellular localization and translocation during apoptosis. Cell Death Differ 1999 ; 6: 644–651.
20. 20 Mancini M, Nicholson DW, Roy S, Thornberry NA, Peterson EP, Casciola‐Rosen LA, Rosen A. The caspase‐3 precursor has a cytosolic and mitochondrial distribution: implications for apoptotic signaling. J Cell Biol 1998 ; 140: 1485–1495.
21. 21 Kamentsky LA, Burger DE, Gershman RJ, Kametsky LD, Luther E. Slide‐based laser scanning cytometry. Acta Cytol 1997 ; 41: 123–143.
22. 22 Darzynkiewicz Z, Bedner E, Li X, Gorczyca W, Melamed MR. Laser scanning cytometry: a new instrumentation with many applications. Exp Cell Res 1999 ; 249: 1–12.
23. 23 Bedner E, Ruan Q, Chen S, Kametsky LA, Darzynkiewicz Z. Multiparameter analysis of progeny of individual cells in clonogenicity assays by laser scanning cytometry (LSC). Cytometry 2000 ; 40: 271–279.
24. 24 Bedner E, LI X, Kunicki J, Darzynkiewicz Z. Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry 2000 ; 41: 83–88.
25. 25 Smolewski P, Bedner E, Du L, Hsieh T‐C, Wu WM, Phelps DJ, Darzynkiewicz V. Detection of caspases activation by fluorochrome‐labeled inhibitors: multiparameter analysis by laser scanning cytometry. Cytometry 2001 ; 44: 73 ‐ 82.
26. 26 Luther E, Kamentsky LA. Resolution of mitotic cells using laser scanning cytometry. Cytometry 1996 ; 23: 272–278.
27. 27 Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide‐ranging implications in tissue kinetics. Br J Cancer 1972 ; 26: 239–257.